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We narrowed to 642 results for: Emb;

Showing: 251 - 300 of 642 results

  1. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...capsid (Cap) genes necessary for the production and assembly of viral capsids. The capsid protein encoded in...proteins necessary for AAV vector production and assembly. The capsid protein encoded in the packaging plasmid...SYBR Green Technology Content last reviewed: 10 September 2025 Do you have suggestions for other plasmids...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly...split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of a “Top” and ...fluorescent split aptamer for visualizing RNA–RNA assembly in vivo." ACS synthetic biology 6.9 (2017): 1710...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...devised a “Modular fluorescence unit fused with TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA...they observe fluorescence localizes to the plasma membrane only when the RTK is activated and the ITAM phosphorylated...relies on a change in localization to the cell membrane and not a direct change in the fluorescent properties...

  4. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...heads and long legs that protrude from the cell membrane, physically connecting the extracellular environment...the integrin, where it is resisted by the ligand embedded in the extracellular environment. This further...the extended open integrin conformation. During embryonic development, extended-open integrins enable tissue...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and ...and target capture module (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes...loss. Ci Ji Lim’s lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...deposited versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most... modulators of cell signaling pathways during embryonic development and cancer. But there is still much...intestinal peptide (VIP), or lysosomal-associated membrane protein 5 (LAMP5) — as well as cholinergic (choline...

  8. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters could not be used ...Venus fluorescent protein in conjunction with a membrane-inserted voltage sensing domain (LOTUS V; Luminescent...Universal Sensing of Voltage). With this sensor, membrane depolarization increases the BRET signal whereas...

  9. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...committee in graduate school. Thesis committee members provide expertise in designing and interpreting...to introduce yourself to and interact with the members of your field, and present your research. Face ...family. Those people are your support network, so remember to thank them verbally and often for their love...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... scientific mentor and choosing the right lab. Remember that your time is valuable! Don’t jump on the ...to plan out a very distinct and certain path, embracing uncertainty can help you both in your career and....org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 . https://doi....

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...Neurodegeneration Research Collection Addgene has assembled the following to help you find information and...cases having at least one other affected family member. These familial cases, usually inherited in an ...ER contact with other organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein...

  12. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore, certain...detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment,...dynamic samples Ex: 3D cell cultures, small model embryos >15 μm Thsee are some of the most challenging samples...

  13. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody recognizing the protein of...is added, and will bind to that protein on the membrane. Then, addition of a secondary antibody allows...bind to proteins on the cell surface, or the cell membrane can be permeabilized to allow antibodies to bind...

  14. CRISPR References and Information

    Type
    Collection
    ...collection of protocols, materials, and publications by members of the Division of Signaling and Functional Genomics...cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and ssODNs design and...pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9...

  15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... in CRISPR–Cas9. The EMBO Journal, 37(10), e96941. https://doi.org/10.15252/embj.201796941 Pattanayak,...CTD and the entire REC lobe—do not structurally resemble any other known proteins; they are completely ...

  16. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...single stranded, negative sense RNA virus. It’s a member of the Paramyxoviridae family of viruses, which...staining for alkaline phosphatase, expression of embryonic proteins, and promoter demethylation and expression.... Cells can be differentiated in vitro to form embryoid bodies which are compact balls of loosely organized...

  17. Deep Dive: qPCR

    Type
    Blog Post
    ...1), which may be easier to conceptualize. Just remember to switch them back before reporting the data!...use it! Tempting as it may be to skip this step, remember we here at Addgene frown upon statistical laxness...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... Biology 22:169–174 . https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A...Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Richter F, Fonfara I, Bouazza B, Schumacher...Chem Biol 13:443–448 . https://doi.org/10.1021/acschembio.7b00603 Additional Resources “Optogenetics Meets...

  20. Bacterial Expression Systems

    Type
    Collection
    ...periplasmic space between the inner and outer membranes. Targeting proteins to the periplasm reduces bacterial... in plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain...Collection page to find plasmids and kits for efficient assembly of TALEN constructs. The Zinc Finger Consortium...

  21. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...It's December, and that means it's officially the start of Addgene's winter holiday celebrations! We'...with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew Norton and NEBGCE4All...

  22. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ... former Co-director of Science in the News, and member of the SITN podcast team.     Amy Gilson recently...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on...

  23. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...Mark your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday...presented in this blog post includes data until November 9, 2020. Between then and the posting of the blog...

  24. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ... that everything will ship out in time - from assembling the boxes, to inoculating tubes, and to packing...pic.twitter.com/eJbI0lLiGQ — Dr. Joanne Kamens (@JKamens) September 13, 2018 This massive scale reagent sharing led...

  27. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and programming with...great European photo slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed...

  29. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...are coming along at a fast and furious pace. In December, as we've blogged about before, there was the ...via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014 January 30. (Pubmed) ...

  33. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ... former Co-director of Science in the News, and member of the SITN podcast team. You can follow Vini on...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on...

  34. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in...appropriately named as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating louse Cymothoa...

  38. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...all of the Addgene employees, the Addgene board members, vendors, Addgene supporters, and of course, Executive...integral part in its success. The Addgene board members were also recognized for their essential advice...

  39. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...filled with family, food, and travel, and you can’t remember where you put your flow cytometry protocol. Or...Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your protocols, wouldn...

  40. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...participate in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in...the beginning, I spent a lot of time shadowing members of my team to learn what I was to do for my time...

  42. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and higher level gene networks...

  44. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...performed with a probe surrounded by a semipermeable membrane, which is inserted into the region of the brain...passive diffusion of DA across the semipermeable membrane from the extracellular fluid into the perfused...

  45. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...checklist as champagne or cupcakes, or maybe the lab member originally responsible for the deposit had already...workflows and keep things moving internally. And remember, a publication isn’t required to share your plasmids...

  46. An Addgene Summer Internship

    Type
    Blog Post
    ...for the first time, being introduced to all the members around the office one by one, I knew that I belonged...right concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running...

  47. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...

  48. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...came to visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run ..., the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family, including...

  49. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...

  50. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...
Showing: 251 - 300 of 642 results