...contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR (Acr) proteins potently inhibit...picking approach to select cells with variant characteristics they wanted, the team identified Archon1, a novel...CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue light quickly...indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment...vectors (pUC57-L1L4 and pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce...for protein-protein interaction (PPI) studies in plants that rely on transient transfection systems using...Biotechniques 2012. PubMed PMID: 23066669.Hecker A et al, Plant Physiol 2015. PubMed PMID: 25971551. Discovery ...

...IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17/SPF45...IgG2a 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a...applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The...The recombinant monoclonal antibodies or R-mAbs have been engineered such that they are a distinct IgG... by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection The following plasmids...Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4 [N222/6R...

...Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids...well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies in the...Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that recognize common.... ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...

...Averof pBUN6A11 50579 Plant BsaI yes, activate S. pyogenes Bar Chen pBUN6I11 50580 Plant BsaI yes, interfere...Bar Chen pBUN411 50581 Plant BsaI yes, cut S. pyogenes Bar Chen pBUN501 50582 Plant BsaI yes, nick S. pyogenes...Chen pHSN6A01 50586 Plant BsaI yes, activate S. pyogenes Hyg Chen pHSN6I01 50587 Plant BsaI yes, interfere...Chen pHSN501 50589 Plant BsaI yes, nick S. pyogenes Hyg Chen pCBC-DT3T4 50592 Plant BsaI none S. pyogenes... Chen pCBC-MT1T2 50593 Plant BsaI none S. pyogenes Chen pCBC-MT2T3 50594 Plant BsaI none S. pyogenes Chen... Chen pCBC-MT3T4 50595 Plant BsaI none S. pyogenes Chen pBUN421 62204 Plant BsaI yes, cut S. pyogenes ...Bar Chen pU3-gRNA 53063 Plant AarI none S. pyogenes Gao pZmU3-gRNA 53061 Plant none S. pyogenes Gao pU6...

...Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns , and Antares : Fluorescent proteins are ...Renilla luciferase (Nano-lanterns) or NanoLuc® (Antares and Enhanced Nano-lanterns) to allow for BRET-based...Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121...systems. Each type of luciferase has advantages and disadvantages depending on the application. Firefly...Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights Empty Backbones Expression...use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared...Conformationally destabilized Firefly luciferase mutants : A set of three sensors that are increasingly ...

...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...PAM sequence often found in plants, expanding the targeting range for plant genome engineering. Read more...more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk of off-target cutting...like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...-related antibodies including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3 [N202/7R] Anti-Ankyrin-G.... See More Antibodies Find information on Addgene's collection of plasmid-based antibodies, including ...-G [N106/36] Generate anti-kinesin recombinant scFvs . Niwa et al. Cytoskeleton. 2023 Apr 10. Explore ...Explore the recombinant monoclonal antibodies and scFvs for neuroscience research created with high-volume hybridoma... AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic mutations...

...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...

...learn more about antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description ...Find recombinant antibodies targeting neuroscience-related proteins. Antibodies...Neuroscience Antibody Collection Browse or search the table below to find antibodies currently available from...neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific validation...our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-mail [email protected]... Antibodies Neuroscience Neuroscience...Applications PI Do you have suggestions for other antibodies that should be added to this collection? Check...

...that you want to express a gene; however, we have included a function section for if you want to stably...vectors, are studying a different genetic element, or want to express shRNA. Choose by: Species-Specific Expression...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...be functional in your host organism. Host Relevant Promoters Representative Empty Backbones Mammalian...Also see our Bacterial Expression collection page Plant CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) ...resistance marker Also see more empty backbones on our Plant Plasmids and Resources collection page Yeast GAL4... to easily identify your protein using an antibody against that epitope. This could allow you...

...found on this page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 nucleocapsid...research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal...) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid... Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-CoV-2 nucleocapsid...from Ray Owens' lab. Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Abbott...Libraries - Libraries of Spike (S) Ectodomain and RBD mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor ...

...learn more about antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description ...Find recombinant antibodies targeting epitope tags and common fusion proteins. Antibodies... Other Markers Antibody Collection Browse or search the table below to find antibodies currently available...cellular proteins. These ready-to-use recombinant monoclonal antibodies undergo application-specific validation...our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-mail [email protected]... Antibodies Tags and Other...Applications PI Do you have suggestions for other antibodies that should be added to this collection? Check...

...Dynamics of Cytosolic Redox Metabolism in Plants. Plant Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer... potential Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements...that many of the articles linked below include variants with different binding affinities or negative-...turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. Nat Commun... Calcium Yellow protein calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized...genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain... small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 Nov...

...protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant (YPet)...protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant (CyPet)...of a recombinant protein with a 6x His tag. Requires a strain with T7 RNA polymerase. Amp resistant. More...enough to get all the information you need. If you want to dissect an individual protein’s function under...highly controlled conditions in vitro , or, if you want to determine its structure using X-Ray crystallography...lactose/IPTG inducible vector for expression of a recombinant protein with an N-terminal 6x His tag and an ...

...pmScarlet-i_Giantin_C1 Golgi apparatus Giantin mScarlet-I Dorus Gadella 85049 pmScarlet-H_Giantin_C1 Golgi...apparatus Giantin mScarlet-H Dorus Gadella 85048 pmScarlet_Giantin_C1 Golgi apparatus Giantin mScarlet ...mTurquoise2-Giantin Golgi apparatus Giantin mTurquoise2 Dorus Gadella 98880 mNeonGreen-Giantin Golgi apparatus...Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown 11908...protein is often tied to its function, so it's important to determine where your protein of interest resides..., if the marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent protein...Bruchez 44385 pLV-mitoGFP Mitochondria COX8 GFP Pantelis Tsoulfas 80000 pMito-miRFP703 Mitochondria COX8A...

...17798 pM-ErbB4CTF-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17799 pM-ErbB4CTF-PY3 mutant ERBB4 GAL4-BD ALS...delta-kinase-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD... Huntington's Nico Dantuma 23971 VCP(wt)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23972 VCP(R155H)-EGFP... HA CMV ALS Nico Dantuma 23973 VCP(A232E)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23974 VCP(DK0)-EGFP...pHA-deltaN335-Kin1A KIF5A HA CMV ALS Anthony Brown 31607 pKin1A KIF5A CMV ALS Anthony Brown 31717 pRK5M-ADAM10 ADAM10...Babu 128616 Anti-Kv1.1 K+ channel [K20/78R] KCNA1 CMV Episodic ataxia James Trimmer 128627 Anti-Kv4.3 K+ ...telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173759 pET-...

...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids...partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...monoclonal antibodies validated for neuroscience research. The plasmids encoding these antibodies are available...Initiative collection of plasmids, viral preps, and antibodies here. Collections...community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN Initiative...identified by depositors and/or when BRAIN Initiative grants are noted in publications associated with Addgene...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...

...levels mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1 Cre expressed at low levels mutant TEF1 Yeast...levels mutant TEF1 Yeast Piper 60933 pPL5628_TEF1*-Cre_MET15 Cre expressed at low levels mutant TEF3 Yeast... ligand (e.g. tamoxifen) to activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated...alternative Cre recombinases that recognize specific mutant loxP sites, VloxP and SloxP sites respectively....Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding domain CMV Mammalian Green 12497...12497 p274 pCMV-CRE-M-AR(T) CREM fused to mutant AR ligand binding domain CMV Mammalian Green 12529 pBS...fused to HIV-TAT - promotes cellular uptake of recombinant Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc...

...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids... our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications PI Don...Collections Search Plasmids Browse Plasmids Browse Antibodies Ordering and MTA Information Featured Collections...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for...plasmids in our collection! These monoclonal antibodies are produced in-house and undergo application-specific...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...

...knockout )? Generate a specific mutant allele of a gene ( point mutant )? Increase or decrease expression...desired sequence, you may want to consider alternative Cas enzymes ( see Cas9 variants and PAM sequences ).... specific disease or process of interest. Do you want to: Generate complete and permanent loss of gene...Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the transient Cas9 expression. The table...may alter protein function when they occur in important protein domains. For gene editing experiments ... addition to off-target activity , it is also important to consider factors that maximize cleavage of ...assay (for NHEJ repaired DSBs): Provides a semi-quantitative readout of the percentage of alleles that have...