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Showing: 101 - 120 of 340 results

  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...proprietary. These methods also rely on ssDNA ends to match up regions of homology, so they won’t work if the...L-arabinose. These cells can be made and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...quite variable from antibody to antibody or batch to batch. For example, take lysine conjugation, which...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... important step in ensuring quality, as it will catch any errors made during the cloning, sample handling... sequences to visually check for homology and mismatches. Do you have your own best sequencing practices...

  7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Plasmid Name Use a descriptive plasmid name and match the name as it appears in its publication or as ...spreadsheet, save it in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...standard conditions be used when comparing different batches of virus. In addition, some viral preps are very... stored in the freezer. If you plan to freeze a batch of virus for long term use, you may want to consider...

  9. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...very low concentration. So how do we make sure to catch all of these potential mishaps before distributing...suspect is contaminated. For AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method...

  10. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...additionally remove all jewellery as well as that watch you’re wearing before you start. Also, avoid unnecessary...cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods should not ...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...calculations so that your assay output number (RQ value) matches the copy number of your sample. In that case, an...options, you’re likely to find an application that matches your needs. Happy quantifying!  References and...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...following the tried and true process of evolution. Watch this video showing bacteria evolve antibiotic resistance...antibiotic resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will...

  14. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Reddit Science is heavily moderated. I started watching the questions and comments go up early in the ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early in the...

  15. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ... sequencing files, either one at a time or as a batch of hundreds, and indicate the guide RNA sequence...compare up to five editing experiments at a time, and batch analysis, which compares hundreds of samples simultaneously...

  18. Viral Production at Addgene

    Type
    Blog Post
    ...transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...    Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer ...

  19. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...for you to explain how your skills and experience match the job requirements, by giving specific examples... a good candidate, explain how your experience matches the job requirements, and address any unusual circumstances...

  20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...repository) and make it easy to find your perfect match. Choosing a Cas9 for your knockout experiment Generating...resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR Guide...
Showing: 101 - 120 of 340 results