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We narrowed to 499 results for: Ank

Showing: 101 - 120 of 499 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...several human and murine CRISPR pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long...constructs, and complete screening in just 3 days? Thanks to Tyler Jacks’ lab, this 3-day goal is is now ...vectors contain uracil-excision based (USER) sites, flanked by ADH1 and CYC terminators for easy cloning of...revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided targeting...difficult to quantify interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... the interview. Greet each interviewer and then thank them once the interview is complete. Remember, your...you, so be engaged with their conversation. Send thank you emails shortly after the interview to the hiring...took during your interviews to help craft these thank you's. Interviewing remotely Remote interviews are...

  3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...sequencing for quality control for over ten years. Now, thanks to a generous donation by the Hirsh family, we ...completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of a pipeline...labs often provide insert sequences or annotated Genbank files that are useful for more complicated plasmids...

  4. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ... don’t follow through on their promises, Wet-blanket negativist, who can bring down the morale of a ...hostile-aggressives, complainers, know-it all experts, wet-blanket negativists, and micromanagers), while other supervisors...book “How To Win Friends and Influence People.” Thank you to our guest blogger!  Dora Farkas received ...

  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences (R and L...recognized during transposition, genetic cargos must be flanked by the L and R ends - thus, scarless insertions...CRISPR-transposons has yet to be fully realized. Many thanks to our guest blogger Leo Vo! Leo Vo is currently...

  6. MXS Chaining

    Type
    Blog Post
    ...particular structures (Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression... contained in individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules...original pattern of the MCS is regenerated at the flanks of each ligation product, which is the basis for...

  7. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...sequence (Figure 2A). Since you don’t need additional flanking sequences, shRNA plasmids are a bit smaller, with... than miRNA plasmids, which require additional flanking regions comprised of upstream and downstream genomic...between the red boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. ...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...easily be attached with a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical... new putative host cell-binding proteins! Many thanks to our guest blogger Jessica Sacher! Jessica Sacher...Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming...for deletion of the inserted transgenes that are flanked by loxP sites. While there is only a small loxP...donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the PB transposase...

  13. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...decorations, our holiday spirit remains. We would like to thank everyone who participated in our annual #Deckthelab... ever! & they totally succeed! WOW! #deckthelab Thank you to everyone who participated and congratulations...

  16. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...will be acknowledged on the data hub website as a thank you for your contribution to open science.  Please...share your thoughts through our Feedback Form.  Thank you for your participation in this resource! We ...

  18. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...Different Audiences Listen to the SITN Podcast Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani... can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

  19. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time to talk to us and...recent collaboration with Seeding Labs here. Special thanks to our sound engineer/producer, Eddy Page! Edward...
Showing: 101 - 120 of 499 results