...sequencing for quality control for over ten years. Now, thanks to a generous donation by the Hirsh family, we ...completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of a pipeline...labs often provide insert sequences or annotated Genbank files that are useful for more complicated plasmids...

... don’t follow through on their promises, Wet-blanket negativist, who can bring down the morale of a ...hostile-aggressives, complainers, know-it all experts, wet-blanket negativists, and micromanagers), while other supervisors...book “How To Win Friends and Influence People.” Thank you to our guest blogger!  Dora Farkas received ...

...particular structures (Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression... contained in individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules...original pattern of the MCS is regenerated at the flanks of each ligation product, which is the basis for...

...mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences (R and L...recognized during transposition, genetic cargos must be flanked by the L and R ends - thus, scarless insertions...CRISPR-transposons has yet to be fully realized. Many thanks to our guest blogger Leo Vo! Leo Vo is currently...

...large scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby... PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

...easily be attached with a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical... new putative host cell-binding proteins! Many thanks to our guest blogger Jessica Sacher! Jessica Sacher...Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications...

...the promoter to prevent its expression, and is flanked (“floxed”) by two sets of incompatible recognition...molecular mechanisms of the brain in vivo. Many thanks to our guest blogger Alessia Armezzani from genOway...233.39 (1971): 149. PubMed PMID: 4940442. Schnütgen, Frank, et al. "A directional strategy for monitoring Cre-mediated...

...sequence to flank a transgene (homologous pairs) or used different scrambled sequences to flank the transgenes...orientation and one of the unused (loxp) sites will be flanked by two lox sites now in the same orientation. Next...

...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the...

...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming...for deletion of the inserted transgenes that are flanked by loxP sites. While there is only a small loxP...donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the PB transposase...

...decorations, our holiday spirit remains. We would like to thank everyone who participated in our annual #Deckthelab... ever! & they totally succeed! WOW! #deckthelab Thank you to everyone who participated and congratulations...

...first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba...Ahmed, Jiachen Lee, and Jillian Parker! Special thanks to our sound engineer/producer, Eddy Page! Edward...

...researchers pursuing careers in academia. Many thanks to our guest, Wei Leong Chew! Wei Leong Chew is...editing, immunology, and gene therapy.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

...will be acknowledged on the data hub website as a thank you for your contribution to open science.  Please...share your thoughts through our Feedback Form.  Thank you for your participation in this resource! We ...

...nontraditional methods including science art. Outro Many thanks to our guest, Joachim Goεdhart! Joachim Goedhart...follow him on twitter: @joachimgoedhart.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

...Different Audiences Listen to the SITN Podcast Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani... can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

...Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time to talk to us and...recent collaboration with Seeding Labs here. Special thanks to our sound engineer/producer, Eddy Page! Edward...

...Scientific Information Accessible We'd like to thank Professor Cepko for taking the time to talk to us...more exciting discoveries from her lab. Special thanks to our sound engineer/producer, Eddy Page! Edward...

...mentorship in developing career skills. Outro Many thanks to our guest, Crystal Shih. Crystal Shih leads ...follow her on Twitter @ShihCrystal.     Special thanks to our sound engineer/producer, Eddy Page! Edward...

...Viral Service), and educational outreach. Many thanks to Alex Chavez for taking the time to sit down ...enable facile control of DNA and RNA.     Special thanks to our sound engineer/producer, Eddy Page! Edward...