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We narrowed to 502 results for: Ank

Showing: 101 - 120 of 502 results

  1. MXS Chaining

    Type
    Blog Post
    ...particular structures (Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression... contained in individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules...original pattern of the MCS is regenerated at the flanks of each ligation product, which is the basis for...

  2. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... with maximal absorption at 260 nm (Figure 1A). Thanks to the Beer–Lambert law, the instrument can calculate...nm, with a distinct secondary peak around 280 nm thanks to aromatic amino acids like tryptophan and tyrosine...applications and that you use the same solution for blanking your machine!  Finally, many NanoDrop systems ...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences (R and L...recognized during transposition, genetic cargos must be flanked by the L and R ends - thus, scarless insertions...CRISPR-transposons has yet to be fully realized. Many thanks to our guest blogger Leo Vo! Leo Vo is currently...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...sequence (Figure 2A). Since you don’t need additional flanking sequences, shRNA plasmids are a bit smaller, with... than miRNA plasmids, which require additional flanking regions comprised of upstream and downstream genomic...between the red boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. ...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...easily be attached with a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical... new putative host cell-binding proteins! Many thanks to our guest blogger Jessica Sacher! Jessica Sacher...Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming...for deletion of the inserted transgenes that are flanked by loxP sites. While there is only a small loxP...donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the PB transposase...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... packaging genes gag , pol , and env . The LTRs flank all of these required genes, and anything in between...contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging capacity Packaging plasmid... vectors are useful research and clinical tools thanks to their long-term expression of a transgene. Stable...generation of large-scale, high-titer preps. This is thanks to established retroviral production cell lines...-competent gamma-retroviral vectors is very low thanks to the design of the plasmids. The genes used for...

  11. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...decorations, our holiday spirit remains. We would like to thank everyone who participated in our annual #Deckthelab... ever! & they totally succeed! WOW! #deckthelab Thank you to everyone who participated and congratulations...

  14. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...Different Audiences Listen to the SITN Podcast Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani... can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

  16. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...will be acknowledged on the data hub website as a thank you for your contribution to open science.  Please...share your thoughts through our Feedback Form.  Thank you for your participation in this resource! We ...

  17. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time to talk to us and...recent collaboration with Seeding Labs here. Special thanks to our sound engineer/producer, Eddy Page! Edward...

  20. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...Viral Service), and educational outreach. Many thanks to Alex Chavez for taking the time to sit down ...enable facile control of DNA and RNA.     Special thanks to our sound engineer/producer, Eddy Page! Edward...
Showing: 101 - 120 of 502 results