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We narrowed to 636 results for: ESP

Showing: 101 - 120 of 636 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...R bodies can repeatedly extend and contract in response to changing pH, and they can rupture E. coli spheroplasts...that can extend and retract over many microns in response to pH changes without the need for ATP or other...polymerization states. Instead, R body monomers respond to pH changes by undergoing local conformational...like vasculature or extracellular matrices) in response to pH changes. Beyond these applications, purified...enzymes for use as a scaffold that changes shape in response to environmental conditions. More speculatively...

  2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...likely to elicit a strong immune response, factors that may make it especially suitable for in vivo research...to its stringent PAM specificity. This can be especially important for base editing techniques, where ...choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed SpCas9...decreased or similar off-target activity, relative to eSpCas9 and SpCas9-HF across a number of different sites...

  3. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et...different applications. For long-term imaging, especially where photobleaching and phototoxicity are a ...constructs come in very handy. This approach is especially suited to complement optogenetic tools, where...autofluorescence and a high signal-to-noise ratio. Despite the many benefits of Nano-lantern technology, there...there is no external light source. Therefore, especially thick samples may not be appropriate for use ...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand dissociation...ligand to receptor to G-protein, and finally, to a response? Let’s take a look at a few examples with plasmids... labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017). In this technique...receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics is similar...scientists then modified some GPCRs further to respond to specific, pharmacologically-inert, small molecules...

  5. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...many as 30% of all cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations...die from cancer-related complications each year. Despite these grim statistics, new strategies for attacking...the accelerator and brake pedal of a vehicle, respectively. In the case of a mutation that turns a proto-oncogene... has access to all of the latest resources? In response to this challenge, Addgene has launched an initiative...

  6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ..., and China (14%). Spikes in plasmid requests correspond to publications of popular papers. Figure...orders come from the U.S. and Europe, we see a corresponding dip in plasmid request activity around March... paper, we observed that the demand for the corresponding plasmids tended to follow an exponential decay...while 𝜓z(t) multiplies the likelihood by the corresponding factor 𝜙z if t is during event z. The "events...the sum of mean +1 and -1 std for each paper, respectively. The fitted model captures weekend, Christmas...

  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...successful modifications, efficiency can be low, especially for larger modifications. What’s the solution... L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an aTet-inducible gRNA and... ability to multiplex repression. Using a Tet responsive promoter to drive expression of a catalytically...a gRNA are expressed on pRH2502 and pRH2521, respectively. Both dCas9 and the gRNA are Tet-inducible, ...and developed new, more tightly controlled, Tet responsive promoters to avoid leaky activation of the system... enable C-to-T and A-to-G base substitutions respectively. Substitutions seem most efficient when using...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...express our gene of interest. This element is responsible for initiating the transcription of your insert...promoter (RNA polymerase binding site) and operators (response elements). Promoters are about 100 to 1000 base...eukaryotes have muliple polymerases which are each responsible for a specific subset of RNAs. To gain this specificity... phosphoglycerate kinase gene.  Constitutive  Widespread expression, but may vary by cell type. Tends ...acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline or...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...signaling, as well as immune response, tissue repair, and kidney development. Despite their ubiquity and significance...mechanisms, or ascertain which integrins are responsible for specific ligand-binding functions, all key...intracellular signal cascades, and allowing agile responses to mechanical forces in the cell’s microenvironment...shaping of the extracellular matrix. During immune responses, this same conformation encourages immune cell...

  10. A Control for All Seasons

    Type
    Blog Post
    ...generally a sample or group that will have a desired response. For example, if you are studying the effect of...that the testing protocols elicited the expected response and have a baseline with which to compare the ...negative control group does not receive the drug, any response recorded would be due to natural biological variation...Biological replicates help you to determine which responses arise from drug treatment versus and which are... control is critical for data interpretation, especially when changes in protein expression are the experimental...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...available through Addgene (Cat.# 70185 and 70186, respectively). Antares Chu, J., et al. (4), likewise, wanted...) probes. These probes generally consist of a responsive protein (e.g. calcium binding protein) fused ...fused to two fluorescent proteins. When the responsive protein binds to calcium it changes conformation and... event being monitored. Optogenetic regulators respond to specific wavelengths of light to initiate signaling...binding domain, and NanoLuc® Luciferase. CalFlux VNT responds to changes in intracellular calcium and is useful...

  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...elements (often linked in groups of seven as a Tet Response Element (TRE)), allowing transcription to proceed...to the promoter and turning transcription OFF. Despite their bacterial origins, Tet systems function well...transactivators GALQF and LexAQF, which activate their respective UAS and lexAop sequences but are also regulated...expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun...Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan. “Power tools for gene expression and clonal analysis...

  13. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...community for years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast in ..., helping teams to be successful in their work despite limited resources. These resources become even ... scientific progress depends on our collective response. Addgene stands ready to collaborate with partners... this time. Your input will directly shape our response initiatives as we stand with the scientific community...

  14. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ... Addgene believe in the power of sharing, and especially in keeping the products of science accessible...clinical trial data, serve society and patients, and respect industry concerns.” 5)      In the aftermath of...journal impact factor, study topic, and the corresponding author's country of origin, were taken into ...content providers and participation from many well-respected and high-profile universities. We particularly...

  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...immune response against the capsids or transgenes. Sensory neurons and liver appear especially susceptible...impact of neutralizing antibodies developed in response to the first exposure to AAV9. The exception is...your prep can help reduce the risk of an immune response. Strain-specific tropism in mice Many systemic... in RNA and DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail macaque brain..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology...

  16. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...me a full description of the tasks I would be responsible for, in comparison to the other positions listed... helped me transition easily. Training and responsibilities Through this training, I was gradually introduced...for the independent work on the QC team. I was responsible for helping other scientists on the team ensure...sequencing QC work    In addition to my responsibilities on the QC team, I was able to explore other...

  17. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ... before/after application of NE (10 μM) and corresponding pixel-wise ΔF/F0 heatmaps. White insets indicate...and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked by alpha2-...because alpha2-AR can provide inhibitory feedback in response to norepinephrine release, and clinically-used... deactivated three times faster. This enhanced response time allows for more precise measurement of norepinephrine...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...inactive dCas9 and VP64, respectively. CibN and Cry2 form a heterodimer in response to blue light, which ... pHlash reporter protein include: H+ specific response cytoplasmic retention insensitivity to other ions...   The Gideon Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids...TCSs). TCSs are utilized by bacteria to sense and respond to their environment - typically through a histidine... kinase, which triggers phosphorylation of the response regulator and downstream transcription activation...optimizing expression of both the light sensor and the response regulator. These changes reduced system leakiness...substrate for Renilla luciferase. This approach is especially suited to complement the use of optogenetic tools...

  19. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ... most scientists start out in academia, it is especially important for graduate students and postdocs ...researchers must be extreme self-starters and especially good at self-promoting—No one is going to tell...acceptance can be an issue in getting promoted (especially race and gender) Interpersonal issues can create...first step. Developing mentoring relationships, especially with peer mentors, to discuss your strengths,...

  20. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ... traction.  This can carry a heavy price tag, especially if you're working on something other than software....  Your venture should continuously evolve and respond to feedback throughout its lifetime.  In this way...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...discuss miLab.  Although many emails resulted in no response, I was still able to get through to a number of...
Showing: 101 - 120 of 636 results