...studies. Additionally, Spinach, the first aptamer identified to bind DFHBI, is highly unstable in vivo due...site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute to the instability...Broccoli, the other green fluorescent aptamer, was identified through directed evolution. First, a pool of ... that activate the fluorescence of DFHBI were identified using systematic evolution of ligands by exponential...hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO shares many structural properties...Mango, coined Mango II, III, and IV, were then identified through microfluidic-assisted in vitro compartmentalisation...possibility for scientific advancement, and in a field with as much potential for growth as RNA biology...

...other scientists within their fields than scientists outside their fields Figure 6: Follower Network of... follow scientists within their field rather than outside their field. Lower: Follower networks of subgroups...used in this post were created using data from or modified from Ke et al. 2017. 1. Among scientists, historians...Scientific Disciplines on Twitter. Twitter users were identified as scientists if they had a scientific title ...

... let’s say you identified gene target X for BRCA-deficient breast cancer and identified several candidate...The biomedical field is often concerned with understanding the cause of diseases and how to treat those...requires drugs. While we often think of these two fields as separate, they are deeply intertwined. In this...to their target, or their direct target can be identified if it isn’t known. If a strong candidate emerges... exciting time to be in the biomedical research field! References and Resources References Fellmann, ...

...all you need are some of your favorite proteins purified to carry out your plan. With a little thoughtful...binding protein (MBP) is a 45 kDa tag which can be purified (along with its fusion protein) with amylose resin... prevents insoluble fusion proteins from being purified by GST (maybe try MBP if you are having solubility...Copper, etc.), allowing His fusion proteins to be purified by single step metal affinity chromatography. ...stringent washing. Nonetheless, this tag can be purified with several different resin types and is a fairly...Coligan, J. E., Dunn, B. M., Speicher, D. W., & Wingfield, P. T. (Eds.). (2001). Current Protocols in Protein...

...lens which magnifies the image, then the ocular lens, where the image is further magnified before it finally...4x objective magnifies the image four times the actual size, the 10x objective magnifies it 10 times, ...also 10x, you would actually be seeing a 100x magnified image of that object! Equipment Light Microscope...aperture are in focus. Center the aperture in your field of view and then slowly open the diaphragm until...the stage (and ultimately your slide) until your field of view is centered on a region of interest. If ...

...about science communication, best practices in the field, and even getting into some active science communication...laypeople.  Laypeople are simply non-experts in the field of science you are communicating from. For instance...communication by and to scientists in (roughly) the same field, often through manuscripts, seminars, or posters...to engage students at all levels.      Jargon is field-specific terminology or language. This can refer...refer both to terms that are only used in the field and terminology that is widely used but has a very specific...specific use-case (definition) in a specific field.  For example, while “PCR test” has recently transitioned...

...outside your particular field of study Assess your interest in a different field Outreach activities...Individual Development Plan (IDP) and uses it to match qualified scientists to jobs in industry, academia, and ...brainer! Engaging audiences from across the hall, fields, and other professions increases your chances of... Facebook, etc.) to learn about available jobs, fields, and trends “Never Leave an Empty Seat” at conferences...Communication Networking As the number of qualified scientists entering the workforce increases, EAs...

...plants can be easily identified and have readily observable phenotypes quantified. Fluorescent reporter-based...However, it is important to note that naked and unmodified dsRNA elicits immunogenic reactions that complicate... as the starting material (DNA-based vector or purified RNA) and the initial state of the dsRNA upon activating...target required physical isolation of the visually identified subpopulation. For traditional cell culture, ... Instead, microscopic plants must be visually identified as undergoing RNAi through the fluorescent reporter...Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001...

...products from both. These products are then purified, quantified, and sent for sequencing. Analyzing your...exciting and versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA...taken to ensure that all of these plasmids are amplified and none are lost. Each Addgene library has its...are differently sized, smaller plasmids may be amplified preferentially, skewing the composition of the... overrepresented plasmids (plasmids that were amplified preferentially). You can also rank the plasmids...

... to look to the United Kingdom’s Nuffield Council. While the Nuffield Council has recently published a.... While this claim has yet to be independently verified, the watching world erupted in controversy. Briefly...mitochondrial diseases. The key success of the Nuffield Council’s report is that when it became clear ...the transmission of mitochondrial disease;” The Nuffield Council began their research into both novel and...any other germline editing technique—too. As the Nuffield Council emphasized, the key to moving research...

...necessary to remove iodixanol prior to use of your purified virus. Nonetheless, we recommend performing a ...a buffer exchange before using the purified AAV in vivo . The iodixanol gradient in this protocol is composed...genome containing) particles. On average, iodixanol-purified AAV preparations contain ~20% empty particles....60% iodixanol step Carefully add up to 5 mL of clarified supernatant on top of the gradient. Use 1X PBS...fractions obtained from the 40% phase contain the purified AAV. Option #3 Puncture the QuickSeal tube slightly...vertical black line indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et...the position of the needle for harvesting of the purified AAV using option #1. Right image adapted by permission...

...audiences in other fields, like when collaborating with colleagues in other fields or writing grant proposals... to draw attention and support to work in their field. It can be hugely beneficial to pose these why questions...public talk, ask a friend who works in a different field to listen to you practice.  Ask them what they learned...main goals: To help graduate students in all STEM fields become excellent science communicators. To empower...students to become ambassadors for their respective fields. To help attendees launch new organizations that...

...Microsoft Excel or similar software Reagents Purified antigen Purified recombinant antibody Isotype control antibody...describes how to run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose...Enzyme-Linked ImmunoSorbent Assay (ELISA) against a purified antigen to validate an antibody. This protocol...the Antigen Standard and coat the plate Dilute purified antigen to 20 ng/µL in a microfuge tube and vortex...concentration. Prepare serial dilutions of the purified antigen as follows: 2 ng/µL : Add 100 µL of 20...coated with serial dilutions of human recombinant purified Desmin (blue) or a negative control protein, Apolipoprotein...

... once the correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain not prone... to recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease...and clump together ~3 days post infection. The amplified virus can be harvested once roughly 50% of the...ultracentrifugation to separate rAdV from other cellular debris. Purified high-titer rAdV is then dialyzed and stored at... protein (280nm) absorbance. The ratio for the purified virus should be ~1.3 Quantitative PCR: the number...into account. You're now ready to use your purified, high-titer rAdV in your experiment. Enjoy! References...

..., loci were identified for 61 such diseases. Now single gene alleles have been identified for 4,163, over...tRNA-derived RNAs. Stay tuned for big developments in this field. Nobel Laureates and the ever expanding importance...another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular RNAs (who...shRNA silencing of one circular RNA they have identified resulted in a cell migration defect. They have... has many surprises for the researchers in this field. Resources at Addgene Browse Addgene's RNAi resources...

...specific sgRNAs with Cas9 or Cas9 derivatives. Unmodified Cas9 causes double stranded DNA breaks that commonly... sgRNAs that were designed based on the rules identified in Doench et al., 2014 to predict sgRNA activity...scientists examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved...). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that recruit additional...demonstrated that the Calabrese library substantially identified more hits than the SAM library approach in a ... Calabrese and the ORF overexpression library identified several overlapping genes, most genes were unique...

...attention! They isolated DNA from the sample, amplified the CRISPR locus from the bacterial genome, and...11 cut RNA with unprecedented specificity: when purified, it could be programmed to generate specific cuts...that this new Cas7-11 might be phylogenetically classified as the more complex Class I systems that contain...shedding the complexity of its ancestors. They classified the system as Class I, Type III-E, and then used... several kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed PASTE, which ...these represent a major step forward for what the field can do.    References and Resources References  ...

...your field or even creating a new field. A knowledge gap is an impediment to progress in a field. It is...broadly understandable to scientists outside your field, and interesting. For example, “there is a significant... to your research problem. Creating a coherent unified story and model can help make your ideas easier...people that are outside your lab and outside your field. If they can understand and are engaged by your ...

... recommend taking on a similar position in your field of interest before you dive straight into a full-time...parasitology. However, when I enquired about roles in this field, most jobs needed at least 3 months experience working... me to see the practicalities of working in the field with the most basic equipment and was a tremendously... fully understood why parasitology roles in the field require experience. Working in industry: Microbiology...language. During the recession there were many qualified scientists with experience and a second language...done with robots. Young scientists going into this field don’t really get to do a lot of lab work but instead...

...mentor, since she does research at UK in the same field that I do: prostate cancer. She has guided me in...Program 2014-15, IMP provided mentorship in the field of Biomedicine to 78 undergraduate/graduate students...internationally. As a part of these plans, mentees identified career opportunities in science as well as the...contacts important for their career goals, and identified possible funding sources to help them carry out... IMP mentoring program extends its scope to the fields of Engineering, Economics, and Philology (in addition...        Rocío López-Diego MD, PhD, is a Board Certified neurologist and multiple sclerosis specialist,...