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We narrowed to 667 results for: FIE

Showing: 101 - 120 of 667 results

  1. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...other scientists within their fields than scientists outside their fields Figure 6: Follower Network of... follow scientists within their field rather than outside their field. Lower: Follower networks of subgroups...used in this post were created using data from or modified from Ke et al. 2017. 1. Among scientists, historians...Scientific Disciplines on Twitter. Twitter users were identified as scientists if they had a scientific title ...

  2. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... let’s say you identified gene target X for BRCA-deficient breast cancer and identified several candidate...The biomedical field is often concerned with understanding the cause of diseases and how to treat those...requires drugs. While we often think of these two fields as separate, they are deeply intertwined. In this...to their target, or their direct target can be identified if it isn’t known. If a strong candidate emerges... exciting time to be in the biomedical research field! References and Resources References Fellmann, ...

  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...all you need are some of your favorite proteins purified to carry out your plan. With a little thoughtful...binding protein (MBP) is a 45 kDa tag which can be purified (along with its fusion protein) with amylose resin... prevents insoluble fusion proteins from being purified by GST (maybe try MBP if you are having solubility...Copper, etc.), allowing His fusion proteins to be purified by single step metal affinity chromatography. ...stringent washing. Nonetheless, this tag can be purified with several different resin types and is a fairly...Coligan, J. E., Dunn, B. M., Speicher, D. W., & Wingfield, P. T. (Eds.). (2001). Current Protocols in Protein...

  4. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...about science communication, best practices in the field, and even getting into some active science communication...laypeople.  Laypeople are simply non-experts in the field of science you are communicating from. For instance...communication by and to scientists in (roughly) the same field, often through manuscripts, seminars, or posters...to engage students at all levels.      Jargon is field-specific terminology or language. This can refer...refer both to terms that are only used in the field and terminology that is widely used but has a very specific...specific use-case (definition) in a specific field.  For example, while “PCR test” has recently transitioned...

  5. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...outside your particular field of study Assess your interest in a different field Outreach activities...Individual Development Plan (IDP) and uses it to match qualified scientists to jobs in industry, academia, and ...brainer! Engaging audiences from across the hall, fields, and other professions increases your chances of... Facebook, etc.) to learn about available jobs, fields, and trends “Never Leave an Empty Seat” at conferences...Communication Networking As the number of qualified scientists entering the workforce increases, EAs...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...plants can be easily identified and have readily observable phenotypes quantified. Fluorescent reporter-based...However, it is important to note that naked and unmodified dsRNA elicits immunogenic reactions that complicate... as the starting material (DNA-based vector or purified RNA) and the initial state of the dsRNA upon activating...target required physical isolation of the visually identified subpopulation. For traditional cell culture, ... Instead, microscopic plants must be visually identified as undergoing RNAi through the fluorescent reporter...Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001...

  7. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...products from both. These products are then purified, quantified, and sent for sequencing. Analyzing your...exciting and versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA...taken to ensure that all of these plasmids are amplified and none are lost. Each Addgene library has its...are differently sized, smaller plasmids may be amplified preferentially, skewing the composition of the... overrepresented plasmids (plasmids that were amplified preferentially). You can also rank the plasmids...

  8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ... to look to the United Kingdom’s Nuffield Council. While the Nuffield Council has recently published a.... While this claim has yet to be independently verified, the watching world erupted in controversy. Briefly...mitochondrial diseases. The key success of the Nuffield Council’s report is that when it became clear ...the transmission of mitochondrial disease;” The Nuffield Council began their research into both novel and...any other germline editing technique—too. As the Nuffield Council emphasized, the key to moving research...

  9. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...audiences in other fields, like when collaborating with colleagues in other fields or writing grant proposals... to draw attention and support to work in their field. It can be hugely beneficial to pose these why questions...public talk, ask a friend who works in a different field to listen to you practice.  Ask them what they learned...main goals: To help graduate students in all STEM fields become excellent science communicators. To empower...students to become ambassadors for their respective fields. To help attendees launch new organizations that...

  10. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ... once the correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain not prone... to recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease...and clump together ~3 days post infection. The amplified virus can be harvested once roughly 50% of the...ultracentrifugation to separate rAdV from other cellular debris. Purified high-titer rAdV is then dialyzed and stored at... protein (280nm) absorbance. The ratio for the purified virus should be ~1.3 Quantitative PCR: the number...into account. You're now ready to use your purified, high-titer rAdV in your experiment. Enjoy! References...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ..., loci were identified for 61 such diseases. Now single gene alleles have been identified for 4,163, over...tRNA-derived RNAs. Stay tuned for big developments in this field. Nobel Laureates and the ever expanding importance...another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular RNAs (who...shRNA silencing of one circular RNA they have identified resulted in a cell migration defect. They have... has many surprises for the researchers in this field. Resources at Addgene Browse Addgene's RNAi resources...

  12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...specific sgRNAs with Cas9 or Cas9 derivatives. Unmodified Cas9 causes double stranded DNA breaks that commonly... sgRNAs that were designed based on the rules identified in Doench et al., 2014 to predict sgRNA activity...scientists examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved...). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that recruit additional...demonstrated that the Calabrese library substantially identified more hits than the SAM library approach in a ... Calabrese and the ORF overexpression library identified several overlapping genes, most genes were unique...

  13. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...your field or even creating a new field. A knowledge gap is an impediment to progress in a field. It is...broadly understandable to scientists outside your field, and interesting. For example, “there is a significant... to your research problem. Creating a coherent unified story and model can help make your ideas easier...people that are outside your lab and outside your field. If they can understand and are engaged by your ...

  14. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...attention! They isolated DNA from the sample, amplified the CRISPR locus from the bacterial genome, and...11 cut RNA with unprecedented specificity: when purified, it could be programmed to generate specific cuts...that this new Cas7-11 might be phylogenetically classified as the more complex Class I systems that contain...shedding the complexity of its ancestors. They classified the system as Class I, Type III-E, and then used... several kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed PASTE, which ...these represent a major step forward for what the field can do.    References and Resources References  ...

  15. Finding Your Perfect Job After University

    Type
    Blog Post
    ... recommend taking on a similar position in your field of interest before you dive straight into a full-time...parasitology. However, when I enquired about roles in this field, most jobs needed at least 3 months experience working... me to see the practicalities of working in the field with the most basic equipment and was a tremendously... fully understood why parasitology roles in the field require experience. Working in industry: Microbiology...language. During the recession there were many qualified scientists with experience and a second language...done with robots. Young scientists going into this field don’t really get to do a lot of lab work but instead...

  16. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...mentor, since she does research at UK in the same field that I do: prostate cancer. She has guided me in...Program 2014-15, IMP provided mentorship in the field of Biomedicine to 78 undergraduate/graduate students...internationally. As a part of these plans, mentees identified career opportunities in science as well as the...contacts important for their career goals, and identified possible funding sources to help them carry out... IMP mentoring program extends its scope to the fields of Engineering, Economics, and Philology (in addition...        Rocío López-Diego MD, PhD, is a Board Certified neurologist and multiple sclerosis specialist,...

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...interneurons using a viral approach Genetically modified organisms like transgenic mice are important tools...efficiency. To further improve specificity, they purified HF-BE3 protein for delivery in ribonucleoprotein...comprised of 2 plasmids, pPSU1 and pPSU2, that can be amplified affordably in bacteria. They are compatible with...strain and contains knockouts for all 3984 genes identified as non-essential. The knockouts replace entire...the arrayed knockdown library targets 289 genes identified or proposed to be essential in B. subtilis 168...systems, which include S. pyogenes (Spy) Cas9, been identified. In Rauch, et al. 2016 the Joseph Bondy-Denomy...inhibit CRISPR function. These Lmo proteins were identified using a bioinformatics approach examining genomes...

  18. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...biomedical research". The family entered the biomedical field’s spotlight after researchers discovered heavy-chain...fragment was exciting, and it wasn’t long until the field was off and running with camelid-derived single-...out of reach and unappealing to many labs), the field is moving towards using synthetic single-domain ...al., 2021).      Figure 2: Genetically modified mice offer an alternative method to producing ... et al., 2021.   The single-domain antibody field has come a long way since the serendipitous discovery...

  19. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...for “technical or product support specialist,” “field application scientist,” or “scientific or technical...career, promote career growth, and/or switch your field of work, both in academia and in industry. Advancing...Advancing along a career path in these two fields looks different however. In industry the career ladder ...Working on a variety of projects and in different fields of research is one of the best parts of my career... with a master’s. While it's possible to change fields of research with a PhD, a master’s is less specialized...

  20. Advice for Choosing a Research Project

    Type
    Blog Post
    ...understand where the field is today? Have you talked to veterans in the lab or the field? What are possible...paper look like? What will be the impact on your field? You want to choose a research question that people...approach with another in a way not done before in your field?” He points out to “not be shackled by your own ...end point of training is not to become a pro in a field, but to be trained to think and perform like a scientist...
Showing: 101 - 120 of 667 results