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We narrowed to 342 results for: GAI

Showing: 101 - 120 of 342 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...negative selection marker HSV-tk is used to select against random recombinants.   Design your targeting...recombination, the negative selection marker selects against improper, random recombination into a different...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...comfortable with the open-ended nature of research, they gain confidence and learn to work independently, which...External collaboration results in student learning gains and positive STEM attitudes in CUREs. CBE Life Sciences...

  4. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... possible to screen libraries of bait and prey against each other at the same time in order to try and...reporter gene in eukaryotic cells, fusion proteins must gain access to the nucleus. To circumvent this requirement...

  5. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... turned to characterizing its cleavage pattern. Again, they were in for a surprise! Whereas Cas9 generates... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR. This “second chance” mechanism...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies...middle panel). Samples were also immunoblotted against vinculin as a loading control (right panel). Image...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...eluted.     In the first method, an antibody against a target protein is immobilized, or tethered, on...variation, called a pull-down assay, an antibody against the tag is used to isolate the protein of interest...

  9. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... code, we may need additional clarification and again we collaborate with the Product Team who in turn...would be like re-inventing the wheel over and over again for every single software today that prints labels...

  11. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...giving you a plethora of opportunities to explore! Again, attending one of these fairs is a great start, ...sure to get that person’s contact information and again, make a personal connection! Even if you don’t get...

  14. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...visualize their target. These antibodies are generated against a specific antigen and then conjugated with a fluorescent...that you will be able to image them in the end. Again, the methods you use will depend on target and sample...

  16. March for Science

    Type
    Blog Post
    ...then revise their hypotheses and the cycle begins again. No part of this process is inherently political...scientists’ ability to uphold their side of the bargain and do the research required to inform the public...

  17. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...2015) or the UnaG protein that binds bilirubin (Kumagai, 2013). Next to these naturally occurring co-factor...biology 27 (2015): 39-45. PubMed PMID: 26087123. 4. Kumagai, Akiko, et al. "A bilirubin-inducible fluorescent...

  18. A Control for All Seasons

    Type
    Blog Post
    ... experimental (?) groups were run on a western against a protein standard (P) to check for tau molecular...antibody specificity is to test the primary antibody against wild-type and knockout samples. In this test, wild-type...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...light-driven pumps that pass ions (Cl-, H+, Na+) against the concentration gradient and hyperpolarize the...activation inhibitory (ArCH) proton pump passing ions against the concentration gradient. Created with BioRender.com...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov...Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator Correlates...
Showing: 101 - 120 of 342 results