We narrowed to 116 results for: Gla

...HA-Parkin PRKN HA CMV Parkinson's Ted Dawson 17706 GLASTp-DsRed2 SLC1A3 DsRed2 Episodic ataxia Nicholas Gaiano...Wade Harper 23026 pET9d-hnRNP-A1 HNRNPA1 T7 ALS Douglas Black 23050 pDEST26-hOPTN OPTN His CMV ALS Jon ...puro)-Omi-mCherry HTRA2 mCherry LTR Parkinson's Douglas Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ...

...and plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston University have greatly...

...fragments at a time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells... window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...

...your skin, including, but not limited to broken glass or needles. Sharps containers must be thick walled...Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves. This lab coat should...

...Strain Vendor(s) Genotype BL21 Invitrogen; New England BioLabs E. coli B F dcm ompT hsdS(rB mB) gal ccdB...proAB) [F traΔ36 proAB lacIqZΔM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (...amplified. These DNA fragments are attached to a glass slide so that different fragments of DNA, or templates...

...Materials Material Vendor and catalog # AgeI New England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer... at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be decontaminated...

... be used as needed. For BSL-2 work always wear glasses/goggles in addition to the BSL-1 requirements. ...

...UV box, it is a good idea to place the gel on a glass plate if available. Unlike the plastic tray, this...

...commercially available restriction enzymes, see New England Biolabs' website . Last Upload: May 18, 2018 Protocol...

...mL of LB, weigh out the following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract ...

...concentration of 10 mg/mL) Gel loading buffer, New England Biolabs B7022S Procedure Pouring a Standard 1% ...

...Potassium Acetate) 120 mL 5M Potassium acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III ...

...enzyme buffer (see (Link opens in a new window) New England Biolabs for more information about restriction ...

...applications. CIDAR MoClo Parts Kit Bacterial Expression Douglas Densmore A collection of 93 modular plasmids and...

...doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering...

...helper adenovirus: production of gutless adenovirus (GLAd) free of adenovirus and replication-competent adenovirus...