We narrowed to 310 results for: Gnas

...rounds of stripping and reprobing, maximize your signal by starting with the least-abundant protein and...your fluorescent probe. Then, if you don’t see any signal, you’ll want to check for the primary antibody ...that you’re looking for a “blank” result! If some signal remains, you may want to repeat your stripping ...

...FRET signal. Subsequent demethylation separates the fluorescent units and so lowers the FRET signal back...a large role in turning genes ‘off’ and ‘on’, signaling which genes should be ‘read’ by the cellular machinery...

...or above 650 nm (far-red or near-infrared), as signals below 650 nm are highly absorbed by hemoglobin ...hemoglobin and cause undesirable background signals. Many near-infrared fluorescent proteins are derived from bacterial...

...mark pixels used to measure signal along the dendrite. D) Fluorescence signal as a function of the distance...profiling or interrogation of context-dependent signaling in primary cells. The authors used this platform...

...identification of signaling pathways with potential to confer anticancer drug resistance." Science signaling 7.357...Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its major appeal is that it enables the...

...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources on...90746-4 Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell...

...is continuously detected via a fluorescent signal. The signal increases proportionally to the amount of...concentration of the starting template DNA the fluorescent signal throughout the reaction is compared to a standard...

...decreasing the fluorescent signal from the first FP and increasing the signal from the second FP. Several...Addgene has many of these constructs and they are designated mEGFP, mVenus, etc. A word of caution is needed...

...immunodetection tools is their strong signal strength. This signal strength is due in large part to their... be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (...

...proteins, promoters, enhancers, polyadenylation signals, inducible gene-expression sequences, and more....promoter (to drive high-level expression) and a polyA signal (to terminate transcription) and the four constructs... containing multiple identical polyadenylation signals or promoters Gibson et al., 2009 MXS-Chaining...

...Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute... study also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect...transform zygotes into adults and explain how signaling pathways control this process. Additional zebrafish...

...mScarlet-I3 also offered clearer signals due to reduced background signal when tested in more highly autofluorescent...external light source, they have lower background signal and can be used in light-sensitive systems. Most...

...fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise ratios with such techniques can be problematic...fluorophore and subsequently leading to a positive signal. The same effect can be achieved for a fluorophore...than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...

...new improvements. The new variant has increased signal-to-noise ratio and better ability to record subthreshold...they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that... SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023 under a...

...found that adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can ...biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody...NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm...

... Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with...illustrated the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed... opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather because of the...

...with high specificity for critical axon guidance signaling proteins and growth cone receptors. Developed ...conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection includes...

...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...

...plasmid expressing the engineered tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This ...with the modified codon that is recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase and...plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression...

...PinkyCaMP had better brightness, signal-to-noise ratio, photostability, and signal response upon Ca2+ binding...the MitoTRACER system. NLS = nuclear localization signal; TEVp = TEV protease. Created with BioRender.com...