We narrowed to 407 results for: HRE
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TypeBlog Post...content_id":null,"href":"https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org...
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Targeted Mutagenesis with EvolvR
TypeBlog Post...only changes cytidines or guanines to the other three bases, which limits the level of variation that ...EvolvR. To increase the mutation rate of EvolvR, three additional amino acid changes were introduced to... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...media to help my research and my career. Here are three examples of how using social media has helped me...for using LinkedIn and Facebook. For each of the three social media platforms, there’s some statistics ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post.... The three components described above (envelope, packaging, and transfer) are supplied by three types... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post... Cas9 Do I Choose for My CRISPR Experiment? The three years flew by, but since then, scientists have adapted...the Cas9 nuclease for your experiments, there are three important factors to consider: site accessibility... -
Antibodies 101: ChIP
TypeBlog Post...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me. Each of the three steps need... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...G-protein-coupled receptors (GPCRs)! Aside from these three senses, GPCRs play roles in initiating signaling...transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma (Gγ).... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the ...The first set of 383 constructs went online just three weeks following initial receipt on site. To get... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...corporations in the US and Western Europe have taken three strategic approaches to gender equality over the...withdrawn from the US market because of life-threatening health effects. Eight of these posed greater ... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...a reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken...Picking the right reporter for your RANBody While all three reporters stain cultured cells and tissue sections... -
CRISPRainbow and Genome Visualization
TypeBlog Post...fused to a different fluorescent protein to create three distinct colors. The specificity of these orthologs...number of individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can ... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...Challenges of engineering channelrhodopsins There are three main challenges to engineering channelrhodopsins...off-kinetics correlated with the model’s predictions. Three top performers, named ChRger1, ChRger2, and ChRger3... -
All in a Twist: dsRNA
TypeBlog Post...activated if and when dsRNA levels reach a given threshold within the cell, as is often the case upon viral...Endogenously produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...used amount of working memory resources. There are three types of cognitive load: intrinsic cognitive load...understand and process your information. A class of three year olds is unlikely to be able to follow a YouTube... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...X-linked inherited neurometabolic condition and one of three Cerebral Creatine Deficiency Syndromes (CCDS) that... aortic valve that has two leaflets, instead of three) and acute aortic dissections (a tear in the inner... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...constructs discussed are based on Adenovirus type 5. Three generations of adenoviral vectors The adenovirus...histone-like proteins forming the nucleocapsid. B) Three generations of adenoviral vectors: the first generation... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...College. Students in the course work in teams of three in sections of 15 students. Within each section,...section, teams are assigned different groups of three genes selected from different parts of the Met pathway... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...later, the Sontheimer and Davidson labs discovered three anti-CRISPRs for a type II system in Neisseria meningitidis...highly diverse but most block CRISPR activity one of three ways: Inhibit DNA binding Example: AcrIIA4 blocks... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...which is really important to her. Addgenie Shreya Vedantam with our resident dinosaur! Read her Instagram...Instagram feature here. One of our QC Scientists, Shreya Vedantam, left the bench not by choice, but by ... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...comparing the performance of promoters across these three species, the Roska lab concluded that a promoter...that promoter targeting the same cell class in all three species are still greater than random chance. The...