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We narrowed to 433 results for: Hal

Showing: 101 - 120 of 433 results

  2. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which were not accessible...depends on your experiment and method of selection. Shalem and Sanjana say they kept a high representation... CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson...

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...their reporter mRNAs. Using the TRICK plasmids, Halstead et al demonstrated that 91% of reporter mRNAs ...tools for studying translation in living cells. Halstead, et al. Science. 2015 Mar 20;347(6228):1367-671...genes involved in neurogenesis, which had proven challenging using the existing dCas9-VP64 activator. The ...Designing customized fluorescent reporters can be challenging enough, but incorporating multiple reporters ...tools for all-optical electrophysiology A major challenge in the field of all-optical electrophysiology ...destabilizing sequences were added to shorten the half-life of both the luciferase mRNA (3’UTR AUUUA [5...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    ...researchers involved. Communication is a major challenge when researchers are communicating across disciplines...disciplines or doing different tasks. But these challenges can be exaggerated when communicating across ... help other teams successfully overcome these challenges, too. Schedule regular meetings Scheduling a...

  6. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...team called Running for Rare Disease that runs on behalf of the National Organization for Rare Disorders...HOPE; a fitting superpower for those running on behalf of rare disease sufferers like AJ. As the Blue ... First-In-Man studies. Kurt has been running on behalf of AJ Gonzales, a young boy suffering from FOP,...

  7. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary ...translation is known to be important in development, and Halstead et al. used TRICK to examine translation of Drosophila...Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An RNA biosensor...

  10. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...about 65,000 messages and dealing with email can be half of my day sometimes.” He points out that the copious...the influence from his two PhD advisers who were “chalk-and-cheese”. While one was an established Professor....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

  12. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...components of EvolvR and its potential applications. Challenges of existing mutagenesis methods Several continuous...drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...PMID: 30253789. PubMed Central PMCID: PMC6154929. Halperin, Shakked O., et al. "CRISPR-guided DNA polymerases...

  14. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

  15. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...talent pipeline. Turnover at Addgene is about half of what is standard in most sectors. The Addgenies...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...quantification of enhancer activity have remained challenging due to the variable availability of enhancer ...pTALYM3, pTALYM4 Boris Greber pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series ...degredation is the speed at which it works--typical half lives of AID-tagged proteins after the introduction...studying essential proteins. Until recently, it was challenging to apply the AID-tagging system to essential ...expressing and purifying human kinases remains challenging. Small molecule kinase inhibitors usually target...

  17. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...available planarians: Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis....differ. For example, Girardia sp. and Dugesia dorotocephalacan can be fed dsRNA-expressing E. coli that are...

  18. CRISPR in the Clinic

    Type
    Blog Post
    ...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ... enzymes target several strains of E. coli and lethally chop their genomes into many, many pieces. Locus...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...

  19. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies have...treatment appears to be both safe and effective. Challenges for AAV gene therapy Although AAV-based gene ...promising, scientists are working to overcome key challenges to their efficacy:  Pre-existing immunity: Many...

  20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...which creates a challenge when studying them since their complete knockdown is lethal. Incomplete or titratable... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...
Showing: 101 - 120 of 433 results