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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 185 results for: INV-E

Showing: 101 - 120 of 185 results

  1. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...of teachers around the country. How you can get involved with BioBuilder Details for joining BioBuilder...

  2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...gene function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs...vector, L4440, places a target gene between two inverted T7 promoters. Transcription from each promoter...Src homology 3 (SH3) domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis...carry out any biochemical and biophysical study involving protein stability or binding experiments. Potential...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ..., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, D. L., Rodriguez, H., Hiltke, T., Snyder, ...M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, ...not in the iso-genic knockout control. This can involve cell surface labeling and/or intracellular labeling...overexpress the target of interest. This approach often involves the transient transfection of expression plasmids...

  4. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode..., Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki...purchased directly from commercial suppliers without investing time and labour on reagent preparation in the ...

  5. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...can easily sort them by other parameters, such as E-value for protein sequence searches. Additionally,... an evolving database, and all researchers are invited to contribute to it! The CasPEDIA Consortium will...update entries as research progresses, but you are invited to reach out to suggest new entries or even new...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...2016). PubMed PMID: 26814968. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary...gutted”, meaning that all viral genes except ITRs (inverted terminal repeats – the sequences that flank the...a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral ...

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... Hendrickson EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are...the initial cut, the next steps in the process involve repairing chromosomal DSBs. It is important to ...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    .../2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger B, Hensel Z (2019) On the...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ...

  9. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...allow students to propagate the plasmids in either E. coli (ori and ampR) or S. cerevisiae (2µm ori and...answer questions about the evolution of the genes involved in methionine synthesis (5). The budding yeast...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks...

  10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and in vivo...approaches.  Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice ...generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom line on Nanoblades...

  11. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...expansion.  References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A...in cell-free systems. These pathways typically involve multiple steps in which a precursor molecule is...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Let us know about it in the comments or shoot us an e-mail at [email protected] and maybe we can write a ...blog post: Synthetic Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov...aggregation propensity in four expression systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, ...origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the human GM-CSF...nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However, a limitation ...genome-engineering. One particularly attractive application involves using dCas9-activator fusion proteins to induce...a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven challenging ...

  13. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Saloman, J. L., Scheff, N. N., Snyder, L. M., Ross, S. E., Davis, B. M., & Gold, M. S. (2016). Gi-DREADD Expression...a popular tool in the field as it provides a noninvasive method for manipulating cell activity, especially...provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects. Building your...specific circuits. Methods for targeting expression involve tissue specific promoters, AAV serotypes, and the...

  14. Of Myc and Men

    Type
    Blog Post
    ...(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10 displays...Pelham’s first published peptide tagging experiment involved a peptide from the neuropeptide substance P. This...suitable pair for protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization...versions of Myc1-9E10 mentioned above basically involve taking the Myc1-9E10 variable regions and sticking...

  15. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...currently shared amongst the zebrafish community. Don E, et al. Zebrafish. 2017. PubMed PMID: 27631880 Kwan...Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition...improve the study of specific neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected...to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation called...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    ...doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

  18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...Pubmed Central PMCID: PMC1222268. 7. Aronson, Deborah E., Lindsey M. Costantini, and Erik L. Snapp. "Superfolder...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...

  19. CRISPR Guide

    Type
    Collection
    ...36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H... R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, ...edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed by the.... PMID: 26735016 Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, ..., K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013). RNA-Guided Human...Siouve, E., Becavin, C., Depardieu, F., & Bikard, D. (2018). Genome-wide CRISPR-dCas9 screens in E. coli...e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S.,...

  20. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...
Showing: 101 - 120 of 185 results