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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 298 results for: IRES

Showing: 101 - 120 of 298 results

  1. To Each HIS Own

    Type
    Blog Post
    ...Much of today's biological research requires a close examination of specific proteins within a system...no cut or dried answer. Successful purification requires the tag to be accessible to the metal ions once...

  2. RNA Extraction Without A Kit

    Type
    Blog Post
    ... effective at isolating total RNA, this method requires a lot of time and depending on how many samples...without a kit!) As mentioned above, working with RNA requires keeping your samples cold until homogenization...

  4. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...third generation systems such that it no longer requires the viral transactivator, Tat. For more information...unconcentrated prep. With this in mind, if the experiment requires a certain volume of virus, consider scaling up...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... additional sequence (7 kDa). This system also requires less genetic engineering than some of the other.... An obvious drawback of the system is that it requires an additional component to be engineered or expressed...

  8. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...libraries after amplification and after a screen requires the use of next-generation sequencing. Representation...sufficient representation within your mutant population requires a massive amount of cells as starting material...

  9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...being developed. Performing a CRISPR experiment requires delivery of, at minimum, two components: the Cas9...biolistic bombardment, which can have high efficiency, requires specialized equipment, such as a gene gun. Depending...

  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...to transformation (Figure 1). As this approach requires no special reagents or purification kits, it is... (Figure 3). Achieving multiple modifications requires multiple recombination events to assemble the ...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    ... discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits for different purposes...This method takes less than 30 minutes and only requires incubating the columns with 1M phosphoric acid...

  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...transfer plasmids have a wildtype 5'LTR, which requires the presence of the Tat protein to work, while...2nd-generation system because the wildtype 5'LTR promoter requires Tat to function. Third-generation plasmids can...

  14. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral production...efficiency can vary across cell types and generally requires optimization of many variables, such as seeding...

  16. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...and time commitment for users. This method also requires more optimization and controls when developing... of the direct method. Namely, since the assay requires a single conjugated primary antibody it is both...

  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    .... Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume lists ddPCR, so the ...application. Even so, there were examples of great hires who hadn’t submitted a cover letter; their experience...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... tracrRNA and crRNA to cleave target DNA (Cpf1 requires only a crRNA for target recognition and cleavage... PFS. This is in stark contrast to Cas9, which requires that each DNA target have high sequence identity...
Showing: 101 - 120 of 298 results