We narrowed to 298 results for: IRES

...need high-intensity light for activation which requires implantation of fiber-optic cables into the brain...H134R) to function because it’s lower conducence requires higher expression levels in order to activate ...

...third generation systems such that it no longer requires the viral transactivator, Tat. For more information...unconcentrated prep. With this in mind, if the experiment requires a certain volume of virus, consider scaling up...

... additional sequence (7 kDa). This system also requires less genetic engineering than some of the other.... An obvious drawback of the system is that it requires an additional component to be engineered or expressed...

...original CAPTURE method The original CAPTURE method requires the creation of a stable cell line that co-expresses...expression. Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these...

...consider when time is of the essence. This method requires a vector backbone containing a topoisomerase I...expensive of all the options discussed as it only requires PCR and the inexpensive DpnI enzyme. Otherwise...

...libraries after amplification and after a screen requires the use of next-generation sequencing. Representation...sufficient representation within your mutant population requires a massive amount of cells as starting material...

... discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits for different purposes...This method takes less than 30 minutes and only requires incubating the columns with 1M phosphoric acid...

...to transformation (Figure 1). As this approach requires no special reagents or purification kits, it is... (Figure 3). Achieving multiple modifications requires multiple recombination events to assemble the ...

...being developed. Performing a CRISPR experiment requires delivery of, at minimum, two components: the Cas9...biolistic bombardment, which can have high efficiency, requires specialized equipment, such as a gene gun. Depending...

...it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops prior... this saying is especially true. If your event requires a lot of set up or cleanup, ask for volunteers...

...transfer plasmids have a wildtype 5'LTR, which requires the presence of the Tat protein to work, while...2nd-generation system because the wildtype 5'LTR promoter requires Tat to function. Third-generation plasmids can...

...and time commitment for users. This method also requires more optimization and controls when developing... of the direct method. Namely, since the assay requires a single conjugated primary antibody it is both...

... ori and requires the host proteins DnaA and IHF to initiate replication. But pSC101 requires a third ...

.... Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume lists ddPCR, so the ...application. Even so, there were examples of great hires who hadn’t submitted a cover letter; their experience...

...the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral production...efficiency can vary across cell types and generally requires optimization of many variables, such as seeding...

... tracrRNA and crRNA to cleave target DNA (Cpf1 requires only a crRNA for target recognition and cleavage... PFS. This is in stark contrast to Cas9, which requires that each DNA target have high sequence identity...

...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

...composition of the RISC varies, but at the very minimum requires an Argonaute protein. This Argonaute protein is...shRNAs using plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence...

..., BioID2 exhibits enhanced proximal labelling, requires less biotin, and allows scientists to more selectively...APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic to living cells. To circumvent...

...look like (the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great...edits and look for off-target changes. This method requires a set of control cells to compare the sequencing...