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We narrowed to 222 results for: Library

Showing: 101 - 120 of 222 results

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with a relevant...certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, but these...

  4. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular Biology...

  5. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...DNA-based materials, including our popular kits and libraries. This DNA-based materials repository contains ...

  6. CRISPR Guide

    Type
    Collection
    ... lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...lentiviral CRISPR libraries. Figure 8: Overview of genome-wide screening using CRISPR libraries. What Are Pooled...Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous population ..., so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually optimized...activation and repression libraries target promoter or enhancer regions. As each library is unique, be sure ...sure to check the library information and original publication to see if a library is suitable for your experiment...CRISPR libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR...

  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout, activation, inhibition...bases in RNA. Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening....

  9. Hot Plasmids February 2024

    Type
    Blog Post
    ...multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification...

  12. CRISPR Cheat Sheet

    Type
    Blog Post
    ...increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple...

  13. Synthetic Biology - Cloning and Genomic Tools

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    Collection
    ...plasmids related to Cloning and Genomic Tools for library construction, genomic modification, and cloning...: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination Shuttle ...

  14. Cre-Lox and Other Site-Specific Recombinases

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    Collection
    ...Description Gene/Insert Vector Type PI Pooled Libraries Pooled Library Name Type PI Description Mouse Tumor Suppressor...Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase...Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase (SSR) systems, ...

  15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...prime editing efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict...high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled to their target allowing...

  17. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual genes... activator were transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks...

  18. Summer Fun at Addgene!

    Type
    Blog Post
    ... coming soon! We’ll also be building our video library all summer long, with additions to our other video...
Showing: 101 - 120 of 222 results