We narrowed to 527 results for: MEA;

...on the signaling molecule solely asmeans of visualization. This means that if all the primary antibodies...be time to look at the indirect approach - which means it’s time to talk about secondary antibodies. While...require their own incubation and wash steps, which means that indirect methods are often significantly longer...

...this operon can serve as a living biosensor for measuring caffeine content in samples. The Barrick lab and...concentrations in several beverages could be estimated by measuring growth of the ΔguaB pDCAF3 strain. Find the decaffeination...fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence intensity...use to detect PKA activity can be generalized to measure phosphatase activity, second messenger activity...

...facilitated the development of a simple and scalable means of making targeted genetic edits. Cas9 is a DNA .... Cas9’s ability to alternately serve both as a means of modifying DNA and of modulating gene expression...lines or transgenic animals expressing dCas9 as a means of modulating gene expression (6). Altering the ...sites of interest thus represents a straightforward means of exerting tight control over Cas9 nuclease activity...

...manager: yes, being a manager means being someone’s boss. But it does not mean shouting orders and trying...manager with your own team! But what does it really mean to manage a team? If your graduate school experience...just like you are supporting your report(s). This means that you should be able to go to your manager for...

...However, the rate of transfer was variable. They measured transfer rates by comparing the number of colonies...isolated from cheese. Knockdown efficiency, as measured by knockdown of a constitutely expressed RFP reporter...median knockdown of ~40-fold across all species measured. Beyond understanding the basic biology of bacteria...frequency of each of the 40 library strains was measured after 6 and 12 generations with or without induction...

...electrical potential. Thankfully, there is a tool to measure these electrical events! Voltage indicators are...They can be targeted to the plasma membrane to measure membrane potential and can also be directed to ...vitro and a small molecule is added exogenously to measure a phenotypic response in the form of fluorescence...Indicators As previously mentioned, cellular voltage is measured at the cell membrane, and this voltage is particularly...

...mice, AAV is almost entirely non-immunogenic — meaning when conducting in vivo CRISPR screens, AAV is ...types. The combination of serotypes and psuedotypes means you can customize your CRISPR screen to a high degree...improve your selection pool for downstream NGS and means you can use smaller volumes of your library for ...while AAV only has approximately 4.7 kb — which means that when using Cas9, you’re already almost out ...

...associating with one another — which means it cannot be used to measure the dissociation of PIPs. Thus, whereas...ratios) to generate useable data. What’s more, measuring and quantifying FRET is often easier said than...BiFC experiments only require that you image and measure the fluorescence of a single FP. Your control construct...

...their small size and membrane permeability (Figure 2c). These cell-permeable Janelia Fluor (JF) dyes have...small-molecule labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free ... chemistry, small size, brightness, and cell permeability. In past decades, further refinements to this...

... the presence of oxygen during maturation. This meant that the protein could be taken directly from A....can easily utilize GFP-containing plasmids as a means to many functional ends. We’ve listed our favorites...Optogenetics: Scientists can use light to detect, measure, and control molecular signals, cells, and groups...protein to use in your upcoming experiments. In the meantime, if you haven't checked out Gal's blog, you should...

...of life Sometimes, work has to come first. That means you and/or your spouse won’t be at every school ... should be sharing it absolutely equally, which means you only have one-half of the guilt. You can handle...parent(s), the sooner your kids can make a decent meal, fold their laundry, take public transportation,... to dinner this week. One swim lesson missed can mean a world of mental relaxation. And, I’m not sure ...

...But before I do that, let me say that I was by no means an expert on this topic when I set out to write ...managing a lot of references. I cite as I write, meaning I use the software to add the citations in real...write. Things are going to get a little crazy (meaning you are probably going to cite hundreds of references...begin to hate the article. This is normal and it means you’re on the right track!  Get feedback After you...

...guides do not target any site in the genome. This means that while they control for many of the effects ...when really it’s not). In practical terms, this means that using safe-targeting guides as a control will...References 1. Morgens, David W., et al. "Genome-scale measurement of off-target activity using Cas9 toxicity in...Central PMCID: PMC3988262. 4. Hart, Traver, et al. "Measuring error rates in genomic perturbation screens: gold...

...that encode 20 amino acids and three stop codons, meaning that the same amino acid can be encoded by more...avoid certain codons altogether.  So what does this mean for a molecular biologist who wants to express genes...that corresponds to the anticodon sequence. That means there are 61 possible tRNAs. Cells however, may ...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...

...detectable effect on cell viability – meaning there was no measurable cell toxicity – using Cas7-11. This...integration was observed in non-dividing cells, meaning that this system wasn’t dependent on the cell’s...resolved without any toxic effects to the cell. This means almost no indels and no off-target effects, giving...

... in our software. What this means for us is that we can focus on the meat of the software and not waste...or you could choose to do the dishes after every meal and be reassured that your dirty dishes won’t tumble...Python 3.5. Python 2.7 is retiring this year, which means there will be no further updates, security or otherwise...

...the invention of base editing doesn’t necessarily mean researchers can fix any problematic C or A mutations...genetic target is slightly different, so more options mean better chances of identifying a successful editor...all bystander edits produced synonymous changes, meaning the new codon corresponded to the same amino acid...development of genetic therapies even further. In the meantime, base editing will undergo even more improvements...

...They also have a limit of detection of ~5%. This means they do not reliably detect editing events that ...population (Fu et al. 2013, Vouillot et al. 2015). Meanwhile, Sanger sequencing is laborious, time consuming...as .01% (Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that ...the genome. However, their decreased sensitivity means that the best option moving forward may be to integrate...

...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ... the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover...When two fluorophores bleed into each other, it means that the cytometer could detect a certain photon... parameter in the cytometer's software. It also means we should exclude APC-Cy7 from the rest of our panel...

...Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range of cell...endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus was used, then...three germ layers (ectoderm, mesoderm, endoderm). Measuring pluripotency of mouse cells typically involves...injecting tetraploid blastocysts with iPSCs and measuring their ability to direct the development of a mouse...