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We narrowed to 526 results for: MEA;

Showing: 101 - 120 of 526 results

  1. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...manager: yes, being a manager means being someone’s boss. But it does not mean shouting orders and trying...manager with your own team! But what does it really mean to manage a team? If your graduate school experience...just like you are supporting your report(s). This means that you should be able to go to your manager for...

  3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...their small size and membrane permeability (Figure 2c). These cell-permeable Janelia Fluor (JF) dyes have...small-molecule labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free ... chemistry, small size, brightness, and cell permeability. In past decades, further refinements to this...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... the presence of oxygen during maturation. This meant that the protein could be taken directly from A....can easily utilize GFP-containing plasmids as a means to many functional ends. We’ve listed our favorites...Optogenetics: Scientists can use light to detect, measure, and control molecular signals, cells, and groups...protein to use in your upcoming experiments. In the meantime, if you haven't checked out Gal's blog, you should...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...of life Sometimes, work has to come first. That means you and/or your spouse won’t be at every school ... should be sharing it absolutely equally, which means you only have one-half of the guilt. You can handle...parent(s), the sooner your kids can make a decent meal, fold their laundry, take public transportation,... to dinner this week. One swim lesson missed can mean a world of mental relaxation. And, I’m not sure ...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...that encode 20 amino acids and three stop codons, meaning that the same amino acid can be encoded by more...avoid certain codons altogether.  So what does this mean for a molecular biologist who wants to express genes...that corresponds to the anticodon sequence. That means there are 61 possible tRNAs. Cells however, may ...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...detectable effect on cell viability – meaning there was no measurable cell toxicity – using Cas7-11. This...integration was observed in non-dividing cells, meaning that this system wasn’t dependent on the cell’s...resolved without any toxic effects to the cell. This means almost no indels and no off-target effects, giving...

  8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...guides do not target any site in the genome. This means that while they control for many of the effects ...when really it’s not). In practical terms, this means that using safe-targeting guides as a control will...References 1. Morgens, David W., et al. "Genome-scale measurement of off-target activity using Cas9 toxicity in...Central PMCID: PMC3988262. 4. Hart, Traver, et al. "Measuring error rates in genomic perturbation screens: gold...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ...But before I do that, let me say that I was by no means an expert on this topic when I set out to write ...managing a lot of references. I cite as I write, meaning I use the software to add the citations in real...write. Things are going to get a little crazy (meaning you are probably going to cite hundreds of references...begin to hate the article. This is normal and it means you’re on the right track!  Get feedback After you...

  10. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... in our software. What this means for us is that we can focus on the meat of the software and not waste...or you could choose to do the dishes after every meal and be reassured that your dirty dishes won’t tumble...Python 3.5. Python 2.7 is retiring this year, which means there will be no further updates, security or otherwise...

  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...They also have a limit of detection of ~5%. This means they do not reliably detect editing events that ...population (Fu et al. 2013, Vouillot et al. 2015). Meanwhile, Sanger sequencing is laborious, time consuming...as .01% (Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that ...the genome. However, their decreased sensitivity means that the best option moving forward may be to integrate...

  12. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ... the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover...When two fluorophores bleed into each other, it means that the cytometer could detect a certain photon... parameter in the cytometer's software. It also means we should exclude APC-Cy7 from the rest of our panel...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range of cell...endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus was used, then...three germ layers (ectoderm, mesoderm, endoderm). Measuring pluripotency of mouse cells typically involves...injecting tetraploid blastocysts with iPSCs and measuring their ability to direct the development of a mouse...

  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...enhanced response time allows for more precise measurement of norepinephrine uptake throughout the brain...more sensitive than nLightR, both generating a measurable signal after electrical stimulation with a single...single pulse, while nLightR gave a measurable signal not at one pulse, but at the second test point, ten...

  15. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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    Blog Post
    ...connections with coworkers and doing something meaningful with my career. I can’t pinpoint exactly when...that had never been done at Addgene before. This meant I was responsible for establishing a new report ...present, I thought I would be extremely nervous (I mean I WAS presenting to three department leads, I probably...

  16. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ... first need to decide the best way to track or measure the process you’re interested in. Depending on ...examine, i.e. for an enzymatic reaction, you could measure consumption of a substrate OR production of a product...molecule biosensors Addgene has many sensors that measure the concentration of small molecules like arabinose...

  17. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...-life balance. It is harder to manage when the measure of work is not just time spent at the desk, but...This requires a focus on deliverables. It does not mean a manager must allow his team to work mostly at ...relationships with clients and colleagues Sometimes this means you have to fire a good performer if they are enough...

  19. The MTA at Addgene

    Type
    Blog Post
    ... a form needs to be signed. But the truth is, meaningful progress depends on things being organized, tracked...forward new discoveries and developments.  MTAs also mean that everyone is on the same page as to how the ...becomes available. Our one-time deposit agreement means we only have to work with one tech transfer office...

  20. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...increases exponentially with an increase in titer. That means that once you drop below a certain (very high) threshold...concentration to avoid over-diluting, and make sure to remeasure your titer after diluting to confirm. Luke ...Titers: Where do they come from and what do they mean? AAV Vector Quality Control: Going the Extra Mile...
Showing: 101 - 120 of 526 results