We narrowed to 418 results for: Numb

...repeats (Ribeiro et al., 2009), and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research...larger plasmids are also maintained at lower copy numbers within bacterial cells, and bacterial cells with...Summers DK, Sherratt DJ. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant...

...gold-standard” for LOF studies, the high gene copy number found in plants makes traditional knockouts unattractive...reversibly exert their effect independent of gene copy number. RNA interference (RNAi) is a conserved eukaryotic...thereby limiting CRISPR’s effectiveness at high copy numbers and genomic loci with low accessibility. Furthermore...

...-containing cells versus total cell number to determine the number of B cells in that sample. Alternatively...called an event, and assigns each event to a channel number based on intensity. Higher intensity events are...

...knockout library is available alongside a growing number of CRISPR libraries from Addgene’s repository that...by utilizing pre-assembled trimers to reduce the number of cloning steps. The FusX system was verified ...like CLIP require a specific antibody and a large number of cells to analyze. A new technique from Michael...different promoters in the kit and by altering the number of tandemly expressed gRNAs respectively) and 2...

...an average indel frequency of only 1.1%. These numbers are a vast improvement over Cas9-mediated HDR for...editors with distinct PAM sequences, expanding the number of available target sites for base editing (Kim...BE3 editing window variants   Greatly expands the number of target loci for base editing Rees et al., ...

... dates back to 1975 and is based on the average number of citations for recent articles. Of course, a ... research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649    ...

... system that generates vectors containing high numbers of gRNAs in one overnight reaction. STAgR is highly...customizable as vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper...

...organizational skills provide great training for a number of alternative careers in science. In return, the...to expand the conference to accommodate a larger number of attendees. This first-year event’s success is...

...At first, the numbers I presented at biweekly meetings didn’t seem much more than numbers. As time passed...

[email protected] Entries include accession number, species, and assigned category (cancerous, hybridoma...interest. You can filter CLASTR results by minimal number of markers and similarity score match. This tool...

...action that can result in the initiation of a vast number of signaling cascades, inducing different cellular...for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches. They first...

...to assess purity. For labs that produce a small number of AAV preparations this level of quality control... viral vector core facilities producing a large number of viral preparations in parallel, there is a very...

...expression of these three genes with different copy numbers, producing dozens of color hues (Livet et al., ...fluorescent proteins is only possible at low copy number ranges, resulting in low fluorescent protein level...

...anaerobe Facultative anaerobe Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon... cells.   Applications for K. phaffii There’s a number of different uses for the phaffii-lous strain. ...

... authors for additional information (accession numbers, reagent details, author contributions, etc.) before...priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for data management...

... Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids...

...processes. Chemical effectors are suboptimal for a number of reasons, including their potential toxicity ...factors. These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded chromosomally...

...traces corresponding to the dashed box above. The number of action potential (AP) spiking events are indicated... Averaged fluorescence responses to increasing numbers of evoked APs in neurons expressing OCaMP or jRGECO1a...

...Many fluorescent proteins are available and the number of fluorescent proteins is steadily increasing....normalized to the value of EGFP). The higher the number, the higher the theoretical brightness. The theoretical...

...developed assays to test large numbers of cancer variants across a large number of genes as systematically...