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We narrowed to 416 results for: Numb

Showing: 101 - 120 of 416 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...’s wise to quickly assess whether a significant number of cells have been edited. For many experiments...population. You can additionally calculate the minimum number of clones you would have to screen to identify ...occur, allowing you to Sanger sequence a manageable number of the higher probability locations. If you’re ...

  2. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...Unfortunately, even when universities have simple postdoc numbers sorted out there can be gaps in knowledge of demographics...and some that stuck out to me were: Decrease the Number of Postdocs De-incentivize going into PhD programs...services based on what they're spending. Increase the number of science masters programs to prepare scientists...

  3. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...Escherichia coli bacteria. It’s hard to count the number of different commercial strains of E. coli currently... If you were to include customized strains, the number is probably in the thousands! The goal of this ... (F-) contain the fertility plasmid. A low copy-number plasmid, encodes proteins needed for bacterial ...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...a potential limitation in a study. Strength in numbers If you assess your off-target frequency to sit ...whatever your editing event outcome is at a reasonable number, the likelihood of an off-target confounding your... the same principle holds true - the higher the number of distinct editing events, the lower the likelihood...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...achieved by traditional pharmacologic methods. A number of strategies have been developed for utilizing...cellular activity and physiology. However, there are a number of factors that can wreak havoc on your experiments...neuronal signaling or cancer pathways there are a number of optogenetic tools now available to help light...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...repeats (Ribeiro et al., 2009), and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research...larger plasmids are also maintained at lower copy numbers within bacterial cells, and bacterial cells with...Summers DK, Sherratt DJ. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant...

  7. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...gold-standard” for LOF studies, the high gene copy number found in plants makes traditional knockouts unattractive...reversibly exert their effect independent of gene copy number. RNA interference (RNAi) is a conserved eukaryotic...thereby limiting CRISPR’s effectiveness at high copy numbers and genomic loci with low accessibility. Furthermore...

  8. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...-containing cells versus total cell number to determine the number of B cells in that sample. Alternatively...called an event, and assigns each event to a channel number based on intensity. Higher intensity events are...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...knockout library is available alongside a growing number of CRISPR libraries from Addgene’s repository that...by utilizing pre-assembled trimers to reduce the number of cloning steps. The FusX system was verified ...like CLIP require a specific antibody and a large number of cells to analyze. A new technique from Michael...different promoters in the kit and by altering the number of tandemly expressed gRNAs respectively) and 2...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...an average indel frequency of only 1.1%. These numbers are a vast improvement over Cas9-mediated HDR for...editors with distinct PAM sequences, expanding the number of available target sites for base editing (Kim...BE3 editing window variants   Greatly expands the number of target loci for base editing Rees et al., ...

  12. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ... system that generates vectors containing high numbers of gRNAs in one overnight reaction. STAgR is highly...customizable as vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper...

  13. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...organizational skills provide great training for a number of alternative careers in science. In return, the...to expand the conference to accommodate a larger number of attendees. This first-year event’s success is...

  16. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...action that can result in the initiation of a vast number of signaling cascades, inducing different cellular...for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches. They first...

  18. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...expression of these three genes with different copy numbers, producing dozens of color hues (Livet et al., ...fluorescent proteins is only possible at low copy number ranges, resulting in low fluorescent protein level...

  19. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...anaerobe Facultative anaerobe Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon... cells.   Applications for K. phaffii There’s a number of different uses for the phaffii-lous strain. ...

  20. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... authors for additional information (accession numbers, reagent details, author contributions, etc.) before...priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for data management...
Showing: 101 - 120 of 416 results