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We narrowed to 562 results for: PAC

Showing: 101 - 120 of 562 results

  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...based on the location of the ‘A’ relative to the protospacer adjacent motif (PAM). ABE7.10 can edit target...'A' if it lies in a small genomic window: at protospacer position 4-8 counting the ‘NGG’ PAM as 21-23 ...closer to the PAM, i.e., positioned at 8-10 in protospacer, ABE6.3 or ABE7.9 are better suited (Gaudelli...two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven...tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than one genomic...placed in 2-6 bp and 4-8 bp, respectively, of the protospacer. And if the target cytosine is closer to the ...depending on the location of your target base in the protospacer sequence. If you do not find a suitable ‘NGG’...

  2. Lentiviral Prep Service

    Type
    Collection
    ...details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled CRISPR...lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR...

  3. Viral Vectors

    Type
    Collection
    ...host genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses...type, linked above. Virus Expression Genome Packaging Capacity Virus Size (nm) Cells Infected Target Cell...

  4. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ... Angela DePace has put personal development at the center of her lab (pictured above). DePace holds yearly...through thoughtful planning and honest feedback DePace’s yearly planning meetings revolve around two simple...tone of the meeting is almost more important. As DePace says, science is full of “terrible perfectionists...have tangible benefits for both PI and trainee. DePace and her lab members have noticed that the meetings... Park J, Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016...

  5. Control AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ...degrade any residual DNA carried over from the packaging process. Incubate at 37 °C for 45 min. You are...

  7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...start making AAV:  the packaging plasmid which contains the structural and packaging genes, the adenoviral... what gets packaged into the AAV molecule. What’s outside of the ITRs does not get packaged in the AAV...AAV vector. The cloning capacity between the ITRs is ~4.4 kb. ITRs are inherently unstable due to their...propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV...

  8. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... Viral Vector Packaging Service Penn Vector Core Transfer Viral Vector Packaging Service: Penn Vector...Optogenetics Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-1-...pAAV-hsyn-Jaws-KGC-GFP-ER2 Optogenetics Ed Boyden AV-9-20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-9-...-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-5-20949P 20949-...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... authors were exploring were space and robots. Warping to stars in space ships and the rise of killer ...How has scientific progress in genetic editing impacted the world of fiction and vice versa? We draw a...Into Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath of Khan (1982)... combined the worlds of genetic engineering and space travels. The movie sees the USS Enterprise crew ...science.1258096 Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher...//doi.org/10.1085/jgp.36.1.39 Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR (2016) Programmable editing...

  10. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...understanding how this molecule impacts neuronal circuits. We recently packaged a constitutive GABA sensor ...are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes. This collection is now complete... these vectors to more serotypes. We currently package four variants of these biosensors, which are optimized...for manipulating specific neurons. We recently packaged a soma-targeted opsin from Christopher Harvey’...Viral vectors coming soon! These vectors should be packaged and available as AAV in the next few months. Email...

  11. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ... from. Lentiviruses have slightly larger packaging capacities than gamma-retrovirus, coming in around ... viruses are roomy: some adenoviruses pack up to ~35 kb of space for cargo! Adenovirus is an effective... cargo into a cell. These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs...direct application of virus. However, the cargo capacity of AAV is ~4.7 kb, almost half that of lenti and...

  12. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... Depending on the position in the protospacer, the editing capacity of the ABE8s ranged from 1.5x higher...site in the genomic DNA to contain a specific protospacer adjacent motif (PAM) made up of 5′-NGG-3′ (“N...ABE8s demonstrate an overall improved base editing capacity, even at sites that used to be difficult to target....  References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...Edwards A, Gehrke JM, Lee S-J, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella...

  13. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...sequence all the DNA packaged inside the viral particles (Figure 1). Briefly, packaged DNA is extracted from... the helper plasmids or the cell genome can be packaged inside the vectors during production. These impurities... to determine the identity and serotype of the packaged DNA and look for potential contaminants. The ...mentioned earlier, it is common to find DNA from the packaging cell genome, bacterial genomes, cloning vectors...the recombinase for transgene expression. Once packaged in AAV, this pre-recombined DNA could lead to ...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Fisher 26402 pF141 pAcGFP1 SOD1WT SOD1 GFP CMV ALS Elizabeth Fisher 26403 pF142 pAcGFP1 SOD1 A4V SOD1 GFP...Fisher 26404 pF143 pAcGFP1 SOD1G37R SOD1 GFP CMV ALS Elizabeth Fisher 26405 pF144 pAcGFP1 SOD1G85R SOD1 GFP... GFP CMV ALS Elizabeth Fisher 26406 pF145 pAcGFP1 SOD1G93A SOD1 GFP CMV ALS Elizabeth Fisher 26407 pF146...Prnp_SAM2 PRNP U6 Dementia Walker Jackson 79259 pAc5.1B-lambdaN-HA-DmeIF4G-V5His6 EIF4G1 His, HA, V5 Ac5...IDR-mCherry TBP Parkinson's Denes Hnisz 146392 pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G EIF4G His, HA...HA, V5 Ac5 Parkinson's Elisa Izaurralde 146493 pAc5.1B-lambdaN-HA-DmeIF4Ga-Nterm_H EIF4G His, HA, V5 Ac5... Ac5 Parkinson's Elisa Izaurralde 146577 pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I EIF4G His, HA, V5 Ac5 ...

  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the genetic code for reverse transcriptase are packaged (Wu et al., 1997) inside of the capsid . This ...the vector particle, some of the RT protein gets packaged inside of the vector particle, along with its ...-type viral genome can direct the production, packaging, and release of progeny viral particles. And there...4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C., & Telesnitsky, A. (2008). Pseudodiploid Genome... Temin, H. M. (1990). Genetic consequences of packaging two RNA genomes in one retroviral particle: pseudodiploidy...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

  16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...action at a distance.” Through the marvel of DNA compaction, an enhancer can increase the expression of a...millions of base pairs away. (For details on DNA compaction and the structural proteins that make it possible...details. DNA is a 3D Fractal Figure 2: DNA compaction. Graphics courtesy of beyondthedish (left) and...tertiary structures (globules) to achieve exquisite compaction—on the order of 700 terabytes per gram—and form...chromosomes we all know and love. To understand compaction, suppose you have a piece string that’s 10 meters..., are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly distant regions...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...single-strand AAV (ssAAV). To maintain the packaging capacity and improve the specificity of ssAAV, a targeting...CRISPRi screen to identify additional host factors impacting prime editing and found the protein La (a small...proteins and a critical component for viral genome packaging. Addgene recommends both for use in western blots...that was more desirable than having many closely-packed labeled cells. Plus, Voltron2’s fluorescence is...vectors for microglia was further enhanced by packaging the virus as self-complementary AAV (scAAV). However...

  18. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...Gairik Sachdeva. Healthcare consulting is a fast-paced field, requiring people who are willing to quickly...uncertainties and a rapidly evolving healthcare space. The consultants who enjoy their jobs most, get ...typically cutting edge; a consultant might outline the impacts of combining targeted molecular therapies for cancer...consulting can sometimes be challenging. The fast paced nature of projects often means working long hours...more. Essentially any role in the broad healthcare space that can benefit from the blend of business and ...interested in the business of life sciences and it's impact on human health.     Resources on the Addgene Blog...

  19. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...system by modifying paCas9-1 using nMagC714 instead of nMagHigh1-C714, generating paCas9-2. This change did...VVD, N. crassa) (Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion proteins N713-pMag...fold-induction of Cas9 activity (16.4-fold). This paCas9-1 light-inducible system was able to recognize ...(flCas9). When triggered by blue light (470nm), paCas9-1 induced indel mutations via NHEJ (frequency of...their prior work, the Sato lab also showed that the paCas9-2 system could be spatially controlled and reversibly... of gene transcription Nihongaki et al., 2015 paCas9 Magnet proteins from N. crassa Split Cas9, Split...

  20. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.” It is based...antibody performs differently and some antibodies impact the performance of other antibodies. But, once ...the right protocol, multiplex IF can give you impactful and beautiful results. Performing an immunofluorescence...fixation maintains sample morphology with minimal impact to the target epitope. There are multiple methods...exclusive to IF, but they can have a significant impact on the outcome of your IF experiment. Many of the...choose for your experiment will have a significant impact on the protocol you use and the ultimate outcome...
Showing: 101 - 120 of 562 results