We narrowed to 229 results for: PID

1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   ...individual gRNA with WT Cas9, repair levels decrease rapidly 10 bp from the cut site. So if you can’t find a...

2. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

   Type

   Blog Post

   ...where the impact of teaching and doing science is rapid, concrete and open to innovation by all.  Additional...

3. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...especially if that transgene is toxic. Eventually, the rapidly growing low-level transgene expressors may take...

4. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   ... elegans selectable marker systems. This novel, rapid, and user-friendly system represents another leap...

5. 10 Great Guest Posts We're Thankful For

   Type

   Blog Post

   ...to learn how these amazing tools may be used to rapidly diagnose Zika virus infection. Microbiology and...

6. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   ...Datta, Naomi. “Transmissible Drug Resistance in an Epidemic Strain of Salmonella Typhimurium.” The Journal...

7. Viral Vectors 101: Transductions

   Type

   Blog Post

   ...express episomally long-term (AAV). If you perform a lipid-based or electrical-based transfection, most of ...

8. Fluorescent Biosensors for Measuring Autophagic Flux

   Type

   Blog Post

   ...213. PubMed PMID: 18094608. Sargsyan, A., et al. "Rapid parallel measurements of macroautophagy and mitophagy...

9. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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   Blog Post

   ...engineering and synthetic biology is expanding rapidly.  References 1. Kunjapur, Aditya M., Philipp Pfingstag...

10. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    ...systems during experiments – neither ideal for the intrepid researcher. There existed, therefore, a need for...

11. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    ...method is compatible with dead cell staining using propidium iodine (PI), allowing for calcineurin activity...

12. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ...430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR 73520-984 Syringes, 30 mL, VWR BD302832...

13. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    ...needs of most molecular biology labs. Recent and rapid advances in technologies that permit large-scale...

14. Fluorescent Proteins 101: Luciferase

    Type

    Blog Post

    ...reaction compared to using a high-intensity laser to rapidly excite GFP. As a result of the different mechanisms...

15. Science Career Options

    Type

    Blog Post

    ...including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment Meteorologist Research...

16. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    ...medical professionals can genotype the patient and rapidly test the variants in vitro against different drugs...

17. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    ...linked together, but separated from β1-9 they will rapidly recombine with β1-9 strands nearby resulting in...

18. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type

    Blog Post

    ...a few days later we had an idea. And this idea rapidly grew and became Crowdfight COVID-19, an online ...

19. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    ...unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture and result in a high...

20. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    ...differ from currently used ChRs in that they cause a rapid and almost complete desensitization when exposed...