We narrowed to 257 results for: PORA

1. Live and Let Dye: Self-Labeling Protein Tags

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   ...amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to be labeled...

2. A History of Genome Engineering in Popular Culture

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   ...Ridley Scott wonder what would happen if these corporations would extrapolate genetic engineering to exploit...which are genetically engineered by a powerful corporation to serve as slaves.  Meganucleases In 1989 the...

3. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   ...system to modify your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or...Expression of lambda red recombination genes. Electroporation of substrate DNA and outgrowth of bacteria....

4. Special Delivery: Fluorophore Targeting for FRET Studies

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   ...in the ribosome, resulting in site-specific incorporation of the attached fluorophore. However, since ...Ebright, and Richard H. Ebright. "Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated...

5. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   ... adenovirus [5], Sendai virus [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]...differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl...

6. 27 Hot Plasmids from 2016

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   ...their enhanced TANGO arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human...MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA) oligonucleotides...

7. New FLIS Plasmid (April Fools 2022)

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   ...The Frequency Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that...

8. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

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   ...majority of the countries we ship to, first on a temporary and now on a more lasting basis.   Table 1: Popular...

9. New Viral Vectors - Summer 2024

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   ...synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use viral ...

10. Visualizing Your Plasmid Request Data

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    ...dashboard at-will with new data. These features are incorporated in the current dashboard. The dashboard is coded...

11. Interview with Bart Newland, Addgene’s Newest Board Member

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    ...s very varied. There’s IP work to do, general corporate work, and negotiating contracts with other companies...

12. Bringing Sustainable Practices to the Lab: Easy Action Items

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    ... list some easy but effective habits you can incorporate to reduce your lab consumption and increase your...

13. Your Top Requested Plasmid in 2016!

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    ...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...

14. Designer PUF Proteins for Any RNA Target

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    ... genetic circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks...

15. Save Time and Money by Making Your Own Competent Cells

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    ...Transforming chemically competent cells (protocol)  Electroporation (protocol)  Untergasser Lab Transformation...

16. From our Table to Yours: An Inside Look at Lunch at Addgene

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    ...unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style competitions...

17. Six Spooky Science Stories and Halloween at Addgene

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    ... please! At Addgene, we love finding ways to incorporate science themes into our fun activities and Halloween...

18. Seeing Red: Simple GFP Photoconversion

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    ... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ...

19. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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    Blog Post

    ...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

20. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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    Blog Post

    ... Technology Transfer Offices were, at least, temporarily closing, these groups were willing and able to...