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We narrowed to 653 results for: PPL

Showing: 101 - 120 of 653 results

  1. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...your specific application. Consider also that there may be variations within an application. Take immunohistochemistry...choosing an appropriate antibody first look at the application-specific data provided on the manufacturer’s ... a linear antigen tend to work very well for applications that denature a protein to its primary (linear...while the website highlights a variation of your application, a publication successfully used it in a similar...plan to. To secondary or not to secondary?  The application will also determine whether you should use an... as it will help to amplify the signal. Some applications use either direct (primary-conjugated) or indirect... are secondary considerations to validation, application specificity, and experimental considerations ...

  2. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice. Target and application should be considered The first question you may... a strong signal and to work in a variety of applications. The desired specificity of your secondary antibody...available secondary antibodies and found that across suppliers there was a detection bias of IgG2a>IgG2b>IgG1...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...

  3. Antibodies 101: Validation

    Type
    Blog Post
    ...intended application - Remember, just because your antibody performs as expected in one application does ...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...intentionally generalizable and not specific to certain applications or antibody targets. That said, not every strategy...will be suitable for every antibody or every application. The goal is to show that your antibody has been...strategy to use will depend on your intended application and available resources.     Figure 1...confident in your data. This is a good option for applications like immunohistochemistry on human tissues, ... for validating antibodies for immunocapture applications and is amenable to high-throughput analyses,...

  4. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ... non-refundable fee is applied to cover processing costs. Once the application is approved, Addgene then...excited to announce we now offer an export license application for rabies materials! Some of the materials in...international requestors. We also offer export license applications for VSV and SARS-COV-1 plasmids. Export control...will need to provide a brief description of the applicable research project when purchasing export-controlled...export-controlled materials. Addgene will then apply for the license for distribution to the requester’s organization...offered VSV and SARS-COV-1 plasmids under this application process since May 2020. We find this process ...whenever possible, as we cannot expedite the application process.  More information about export control...

  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... tools for familiar applications. But how does one invent a tool for applications that don’t even exist...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible. But ...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that... shows a wealth of potential, and while many applications are still yet to be demonstrated (or invented...developing new microscopy tools for biological applications across a wide range of topics. He embraces open...explore the mechanisms and develop potential applications.  Andrew is a frequent collaborator and is happy...

  6. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... necessary to apply a compensation value between the two channels (Figure 1A). Applying compensation means...your single-color controls as a starting point. Applying compensation to your experimental samples In order... in the plot. A compensation value of five was applied to APC-Cy7 against APC to correct for this bleed...compensation value of 3.7 for APC into APC-Cy7 applied next, which fixed the bleed in this population....each plot.  The general rules of compensation apply to your experimental samples as they did with your...populations and may need to be “comped” first before you apply compensation values to your current channels. A ...plot, and the population does not move even after applying very large compensation values (100+), consider...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...derivatives of its substrate O6-benzylguanine (BG) (Keppler et al., 2003). This engineered hAGT later became...acids 1 Various Many Complicated   Applications Organic dyes are typically both brighter and...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...ligands just aren’t going to work in certain applications — the most common limitation is that certain...area of research. If size is a concern for your application, SNAP-tag, CLIP-tag (both 182 aa), and eDHFR ...

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...the candidate application process, the candidate’s ability to write a grant application through a written...funding opportunity coincided, and they stepped up to apply for a grant which lead to their new career path.... much more than writing. In fact, many grant applications and grant makers have moved away from long written...Now they require grant seekers to use online applications with significant character limits for the written...written portion of the grant application. They have increased their demand for stronger budgets with clear...duties can range from coordinating the grant application and submitting it, to evaluating the capacity...grant readiness, funding opportunity research, application writing and development, project implementation...

  9. Let There Be LITE Plasmids

    Type
    Blog Post
    ...effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann and her colleagues...just how flexible their LITE system could be by applying it to altering gene expression via epigenetics...light retraction. Finally, because light can be applied non-invasively, its use is not restricted to cultured...optogenetic tools." In total, the researchers applied the LITE system to 28 different genes to establish...cellular processes." The LITE system can now be applied to unraveling the genetic and epigenetic players...disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience, their...elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons, she offers...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...FPs can be used similar to GFP-like probes by supplying a single gene to cells. Still in bacteria, which...additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It is important to consider...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...transiently transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...

  11. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...experienced. Finding an antibody that works for your application, specifically detects your protein, is species...and have been widely used across species and applications. In this blog, we will review the most common...not exist, or they don’t exist for a specific application, or they exist but are unreliable for a variety...almost certainly already been optimized for your application. Depending on the tag, directly imaging or tracking...our affinity purification tag blog!).   Applications of epitope tags   The tags A number of...with moderate to high success rates across all applications. You can find FLAG antibodies on Addgene’s catalog... biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. (2003) 60:523-533 DOI: 10.1007...

  12. Finding Your Perfect Job After University

    Type
    Blog Post
    ...received many applications, employers were requesting that only scientists with an MSc or higher apply. It was...scientific research would be very competitive, and so I applied for a summer role as a Research Scientist at the... edge of London to gain relevant experience. I applied for this summer position as I felt I needed more...I had to reassess what I wanted to do and so I applied for a role in Industry. I wanted to get "a real...university to study a Masters of Science I decided to apply to study a Master of Science (MSc) in Human Molecular...virology classes in California and enjoyed them, so I applied for a role at a commercial virology testing company...enjoy helping people and decided to compromise and applied anyway. I later found out the job was with Addgene...

  13. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...single-stranded oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild...proceeds from the maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template...consider that already-tested CRISPR alternatives are applicable in many situations. In the case of hypertrophic... need to be much, much lower before clinical applications could be considered. It is important to consider...consider the question of broader applicability of CRISPR germline editing. Nature News mentions that the...only way to answer questions of efficiency and applicability. Given moral and ethical concerns surrounding...experiments are needed before we can consider clinical applications for germline editing. Although this paper represents...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...re used in a number of research and clinical applications. Most of the time, when someone speaks about...deeper penetration into a tissue, useful for applications like IHC, or be able to access an epitope folded...that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher resolution...antibody’s ‘Y’. They are often used in clinical applications. Fab fragments can be created in lab in situations...Shahied, 2004). They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins...antibodies are still an excellent option for many applications, and frankly, if ain't broke, don't fix it!  ...flexible in your approach to affinity reagent applications. Whatever affinity reagents you use, one thing...

  15. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...between mGold2s and mGold2t depending on the application. Figure 1: Super-resolution imaging...proof-of-concept, they believe that the strategy will be applicable with other TnpBs and in other plant species, ...available for tracking cell lineage, different applications require careful selection or design to ensure...for gene therapy and ex vivo cell engineering applications and is especially well-suited for lung cells...tool for circuit tracing and other neuroscience applications (Figure 4). Check out our blog post on AAV serotypes... Figure 4: AAV6 and AAV11 tropism and applications. A) AAV6 is reported to have broad tissue tropism...to be swapped out, potentially broadening its applications. Find ATLAS plasmids here! Rivera, J. F., Huang...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...your media with supplements such as magnesium salts, buffering agents, and/or supplying additional carbon...are, you’ll even have DNA left over for other applications, like PCR, cloning, transfection, or long-term...wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain...your E. coli to ensure it is suitable for your application. We have previously reviewed many common lab ...culture media Changing to a richer media or adding supplements can improve your cell density, leading to an ...normally recommended antibiotic concentration. Add supplements to the media You can achieve higher cell density...

  17. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...EthoVision. Consult the literature to find all the applications of optogenetics relevant to your field. Ultimately...behavior, certain technical considerations still apply. Testing stimulation First, In lieu of electrophysiology...steps and technical considerations are directly applicable to the rat [6]. Technical differences include...models. Beyond rodents, optogenetics has even been applied to other species such as non-human primates [7,...PMC3595120. 4.Mattis, Joanna, et al. "Principles for applying optogenetic tools derived from direct comparative...rat lines: tools, techniques, and optogenetic application to dopamine-mediated reinforcement." Neuron 72.5...sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex...

  18. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...researchers could apply this technique to efficiently deliver large repair templates for applications that require...with a homologous repair template. It is this application - the creation of sequence-specific knockins ... allele of a single gene at once. Therefore, applications that require simultaneous modification of all...clones, making it unsuitable for most in vivo applications.  Third, even in the most optimized tissue-culture...components as ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery...you’ve been able to check out some of the many applications of AAV in some of our other blog posts and have...

  19. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...is an application in which antibodies that have been joined to fluorescent molecules are applied to a ... Primary Antibody This is the first antibody applied to your sample, which will bind to your target ...Unsurprisingly, this is the second antibody you apply, which binds to the first antibody and is conjugated...distribute antibodies validate them in a variety of applications, including IF (or IHC or ICC), and will share...Resources on the Addgene blog Learn about research applications of antibodies in Introduction to Antibodies ... for Me? Read about another common antibody application in The Basics of Western Blotting Resources ...

  20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...reproducible if its findings can be predictably applied to future work. Importantly, no one should expect...studies, as reported by drug companies attempting to apply the findings from these studies, are quite low (...low rates of reproducibility. Failed attempts to apply irreproducible results to future work cost both ...solutions based on what I’ve learned. I hope you can apply some of these solutions to your own research and...ridiculous to expect that one should be able to apply someone else's new and incredibly useful technique...information about fluorescent proteins so you can best apply these technologies to your own research needs. Other...evidence shows otherwise. Others will go on to try to apply these findings in their own work ultimately wasting...
Showing: 101 - 120 of 653 results