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We narrowed to 658 results for: PPL

Showing: 101 - 120 of 658 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    ...good quality application. Even following this advice, only about 50% of my grant applications have been ...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...alterations before submitting - handing them your application 3 days before the deadline isn’t going to be ...problem in a novel way. Finally, engineering new applications to solve problems will provide originality to...

  2. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...student from applying to the opportunities of their dream.  To help students focus on the application process...internships you are applying to, keep your resume under two pages unless you are applying for a Federal Government...your application a whole week before the deadline. The day of submission, review your application with...COVID-19 pandemic, students will face a reduced supply of internships as a result of the necessary restrictions...Scrutinize every opportunity prior to starting an application to assess your eligibility, interest, and career...February represent peak season for internship applications. Thus these noble individuals are inundated ... with a stellar letter of support.        5. Application submission  Set a calendar reminder for every...

  3. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...usually yes! A good cover letter won’t hurt your application, but it can certainly help you stand out.  In...consider cover letters an important part of the application. Even within an organization, there can be variance...good cover letter!) As with any part of the job application process, it's a good idea to talk to people in...do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume... to proactively address questions about your application. These could be logistical, like briefly explaining...  Finally, it’s a good idea to state why you’re applying to the specific company or lab. Take some time...what goes into your cover letter. Imagine you’re applying to a job with a lot of file management that asks...

  4. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...developed and applied. The current pandemic serves as a striking example of the applicability and importance...research. I applied, hoping that the experience would improve my scientific and application writing skills...science (as opposed to translational, biomedical, or applied sciences), people conduct research to answer questions...revelation. Science in any field can generally be applied to improve our lives and solve global problems....the people around you. Be proactive Rather than applying only to posted job offerings, the best research...ties into the previous one too. I would recommend applying for multiple positions—don’t put all your eggs...and just lurked online for a while and observed. Apply for those scholarships that you don’t think you’...

  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...relationships. However, most job seekers will and should apply for jobs posted online as one part of their job ...Utilizing existing relationships as part of the job application process can dramatically increase the chances... of a resume can be crucial for a successful application. It takes time to prepare an excellent resume... Many job postings will result in hundreds of applicants the day they are posted.  If your resume is not... here for more on timely submission of a job application. 3) Read job descriptions carefully Be open minded...have to have all of the listed qualifications to apply for a position.  A job description is usually a ...wish list and the hiring manager knows that no applicant is likely to meet all the criteria. Note the criteria...

  6. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...received 116,224 employment based green card applications and approved 91,023 during the first 3 quarters...options discussed in this post benefit from the applicant thinking about the process early, keeping good...occupation" as requiring theoretical and practical application of a body of highly specialized knowledge in ... April 1 deadline for companies to submit an application on their behalf.  If an individual obtains a ... cap subject H-1B (last year less than 30% of applicants received sponsorship) then he or she can start...1B petitioners are exempt from the cap if the applicant works at institutions of higher education, a related...NIW) allows for self-sponsorship of the I-140 application meaning that the category does not require a ...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...led to their application for basic and translational research.  Basic research applications of scFvs: ...blood clearance, which is useful for imaging applications; better tissue penetration which is useful for... for therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to...from blood can be a drawback for therapeutic applications where longer retention times often increase ...since it’s genetically encoded.   Translational applications of scFvs: Just like antibodies, there are many...2019).Many of the scFvs designed for oncology applications are reformatted as bispecific molecules which... autoimmune, and/or inflammatory therapeutic applications. Antibody fragments such as scFvs are also used...

  8. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...your specific application. Consider also that there may be variations within an application. Take immunohistochemistry...choosing an appropriate antibody first look at the application-specific data provided on the manufacturer’s ... a linear antigen tend to work very well for applications that denature a protein to its primary (linear...while the website highlights a variation of your application, a publication successfully used it in a similar...plan to. To secondary or not to secondary?  The application will also determine whether you should use an... as it will help to amplify the signal. Some applications use either direct (primary-conjugated) or indirect... are secondary considerations to validation, application specificity, and experimental considerations ...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice. Target and application should be considered The first question you may... a strong signal and to work in a variety of applications. The desired specificity of your secondary antibody...available secondary antibodies and found that across suppliers there was a detection bias of IgG2a>IgG2b>IgG1...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...

  10. Antibodies 101: Validation

    Type
    Blog Post
    ...intended application - Remember, just because your antibody performs as expected in one application does ...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...intentionally generalizable and not specific to certain applications or antibody targets. That said, not every strategy...will be suitable for every antibody or every application. The goal is to show that your antibody has been...strategy to use will depend on your intended application and available resources.     Figure 1...confident in your data. This is a good option for applications like immunohistochemistry on human tissues, ... for validating antibodies for immunocapture applications and is amenable to high-throughput analyses,...

  11. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ... non-refundable fee is applied to cover processing costs. Once the application is approved, Addgene then...excited to announce we now offer an export license application for rabies materials! Some of the materials in...international requestors. We also offer export license applications for VSV and SARS-COV-1 plasmids. Export control...will need to provide a brief description of the applicable research project when purchasing export-controlled...export-controlled materials. Addgene will then apply for the license for distribution to the requester’s organization...offered VSV and SARS-COV-1 plasmids under this application process since May 2020. We find this process ...whenever possible, as we cannot expedite the application process.  More information about export control...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...derivatives of its substrate O6-benzylguanine (BG) (Keppler et al., 2003). This engineered hAGT later became...acids 1 Various Many Complicated   Applications Organic dyes are typically both brighter and...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...ligands just aren’t going to work in certain applications — the most common limitation is that certain...area of research. If size is a concern for your application, SNAP-tag, CLIP-tag (both 182 aa), and eDHFR ...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... tools for familiar applications. But how does one invent a tool for applications that don’t even exist...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible. But ...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that... shows a wealth of potential, and while many applications are still yet to be demonstrated (or invented...developing new microscopy tools for biological applications across a wide range of topics. He embraces open...explore the mechanisms and develop potential applications.  Andrew is a frequent collaborator and is happy...

  14. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... necessary to apply a compensation value between the two channels (Figure 1A). Applying compensation means...your single-color controls as a starting point. Applying compensation to your experimental samples In order... in the plot. A compensation value of five was applied to APC-Cy7 against APC to correct for this bleed...compensation value of 3.7 for APC into APC-Cy7 applied next, which fixed the bleed in this population....each plot.  The general rules of compensation apply to your experimental samples as they did with your...populations and may need to be “comped” first before you apply compensation values to your current channels. A ...plot, and the population does not move even after applying very large compensation values (100+), consider...

  15. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...the candidate application process, the candidate’s ability to write a grant application through a written...funding opportunity coincided, and they stepped up to apply for a grant which lead to their new career path.... much more than writing. In fact, many grant applications and grant makers have moved away from long written...Now they require grant seekers to use online applications with significant character limits for the written...written portion of the grant application. They have increased their demand for stronger budgets with clear...duties can range from coordinating the grant application and submitting it, to evaluating the capacity...grant readiness, funding opportunity research, application writing and development, project implementation...

  16. Let There Be LITE Plasmids

    Type
    Blog Post
    ...effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann and her colleagues...just how flexible their LITE system could be by applying it to altering gene expression via epigenetics...light retraction. Finally, because light can be applied non-invasively, its use is not restricted to cultured...optogenetic tools." In total, the researchers applied the LITE system to 28 different genes to establish...cellular processes." The LITE system can now be applied to unraveling the genetic and epigenetic players...disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience, their...elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons, she offers...

  17. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...FPs can be used similar to GFP-like probes by supplying a single gene to cells. Still in bacteria, which...additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It is important to consider...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...transiently transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...

  18. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...experienced. Finding an antibody that works for your application, specifically detects your protein, is species...and have been widely used across species and applications. In this blog, we will review the most common...not exist, or they don’t exist for a specific application, or they exist but are unreliable for a variety...almost certainly already been optimized for your application. Depending on the tag, directly imaging or tracking...our affinity purification tag blog!).   Applications of epitope tags   The tags A number of...with moderate to high success rates across all applications. You can find FLAG antibodies on Addgene’s catalog... biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. (2003) 60:523-533 DOI: 10.1007...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...received many applications, employers were requesting that only scientists with an MSc or higher apply. It was...scientific research would be very competitive, and so I applied for a summer role as a Research Scientist at the... edge of London to gain relevant experience. I applied for this summer position as I felt I needed more...I had to reassess what I wanted to do and so I applied for a role in Industry. I wanted to get "a real...university to study a Masters of Science I decided to apply to study a Master of Science (MSc) in Human Molecular...virology classes in California and enjoyed them, so I applied for a role at a commercial virology testing company...enjoy helping people and decided to compromise and applied anyway. I later found out the job was with Addgene...

  20. Genetic Code Expansion

    Type
    Collection
    ...single plasmid, with multiple copies of the tRNA. Applications By making small changes in selected amino acids...
Showing: 101 - 120 of 658 results