... may only be compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must...used with targets that are upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA ...

...Within the editing window -50 to +50 bp from the PAM site, CRISPR-X produces point mutations at a rate...

...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...study alpha2-AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is... increased selectivity for norepinephrine over dopamine. All three sensors, however, did display some ...some reaction to high concentrations of dopamine. This cross reactivity will need to be accounted for in ...

...adventure. Many thanks to our guest writer, Pamela J. Hines, PhD! Pamela J. Hines is a Senior Editor at Science...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...French horn, and rhubarb. Follow her on Twitter @Pam_Hines and LinkedIn. Additional Resources on the Addgene...

...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies... different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049...

...experiment, ChRger2 was non-invasively delivered to the dopamine producing neurons in the ventral tegmental area...were then allowed to “self-stimulate” their VTA dopaminergic neurons by triggering a burst of light that ...poked their nose into a hole in their cage. Since dopamine is an important neurotransmitter that promotes...behavior, if the channelrhodopsins activate the dopaminergic neurons in the VTA, this should propel the mice...

...anesthesia. In Cre transgenic mice, they targeted the dopamine (DA) neurons of the ventral tegmental area (VTA...for encoding aversive stimuli in the mesolimbic dopamine system." Neuron 101.1 (2019): 133-151. PubMed ...Juarez, Barbara, and Ming-Hu Han. "Diversity of dopaminergic neural circuits in response to drug exposure..., Norman E., et al. "Optogenetic activation of dopamine neurons in the ventral tegmental area induces ...

...fragment with FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...Cas9 for genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES fragment is actively shuttled...to the nuclear export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS...

...18 (end) - Panel Alex Tucker (Ginkgo Bioworks) Pamela Hines (Senior Editor at Science) Edward J. Hall...billions of dollars (Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel, irreproducibility...journalists and scientific experts discussed by Pamela Hines Minisymposium on Reproducibility Booklet ...

...vector for Sox1 locus CAG Mammalian Okano 153207 pAM-AAV-mSncg-Cre Retinal ganglion cell-specific expression...

...Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether...Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department of Systems Biology at ...

... The strategy relies on several observations: Rapamycin is a membrane-permeable pharmacological agent ...induction heterodimerization of proteins that contain rapamycin-binding domains, such as FKBP and FRB. The cell...membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) and ...

...without a personal message and they may report you as spam or “I Don’t Know This Person.” If that happens a...the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...

...monosynaptic tracing to identify inputs to two major dopaminergic cell populations in two specific brain regions...Whole-brain mapping of direct inputs to midbrain dopamine neurons. Neuron. 2012 Jun 7;74(5):858-73. Pubmed...

... of fluorophores, as seen in studies involving dopamine D2/D3 receptors (Allikalt et al., 2020) Impact... & Weikert, D. (2020). Fluorescent ligands for dopamine D2/D3 receptors. Scientific Reports, 10(1). https...

...Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee,...

...including next-generation biosensors for GABA, dopamine, serotonin, and much more. Check them out! Table...

... :D) engineering E. coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend much...

...preps from recent months: Second generation GRAB dopamine sensors from Yulong Li’s lab are now available...

... and Neil Thompson. Special thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile...