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We narrowed to 364 results for: Pol

Showing: 101 - 120 of 364 results

  1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...single stranded regions with homology to anneal a polymerase, to fill in the gaps a DNA ligase, to seal the...than 200 nucleotides before the annealing and polymerization steps could occur. Secondly, it does not work...instead of using the exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or ...methods that have been described--CPEC (circular polymerase extension cloning) and SLiCE (Seamless Ligation...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters... sensor, membrane depolarization increases the BRET signal whereas hyperpolarization decreases the signal...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are...Na+) against the concentration gradient and hyperpolarize the cell (Mattis et al., 2012). Bicistronic ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they... A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES is an optogenetic tool developed for bidirectional...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...join any fragments end-to-end without the exo/polymerase generation of annealed complementary regions....and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping enzyme superfamily of...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

  5. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...binding to the template strand (strand used by RNA polymerase II for transcription) are more effective for ...editing! Pooran Dewari is a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand...

  6. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    ...Updated March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name!...participation in suggesting names and voting in our poll. Now, we'd like you to meet..... *drumroll* Aavery... out this form here linked below. We’ll keep the poll open until February 28 5PM EST and we’re excited...

  8. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... uses do you foresee for this tool? Howarth: Polyproteins are useful for mechanical study of proteins ...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell ...References: SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture...

  9. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...microelectronic fibers made from thermoplastic polymers that can work with all the bells and whistles....called thermal drawing, essentially where blocks of polymers are heated up and pulled to a desired thickness... small molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     ...

  10. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...

  11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... the reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew...expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction...Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020...

  12. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    .... The topic of unionization continues to be a polarizing one. High-profile universities remain outspoken... panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office Ben Corb - Director... in the intersection between science and public policy. Follow David on Twitter @driglar.   References...

  13. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...advantage of the program. Start the program by polling the audience with a few questions. Who is out there...in response. I am always ready with some written poll questions to get things started. Give a couple of...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect...

  14. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of...topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When mixed, topoisomerase I... could also be a great choice, because the T4 polymerase needed for this method is relatively inexpensive...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...purified for use in molecular biology research (eg. polymerases and other enzymes might be purified and used ...translation machinery. These include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free...

  16. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Addgene blog   Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview and Applications...Applications Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer...SYBR Green I and TaqMan quantitative real-time polymerase chain reaction methods for the determination ...

  17. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...versus the response and use that information to extrapolate the concentration of an unknown sample. For example...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample based...sample is determined by using the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651...

  18. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...set up a standard PCR reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template...standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard ...heating step of the PCR reaction. A standard Taq polymerase is sufficient. Controls: Controls can make ...

  19. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

  20. Viral Production at Addgene

    Type
    Blog Post
    ...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then ...lentiviral preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated...
Showing: 101 - 120 of 364 results