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We narrowed to 363 results for: Pol

Showing: 101 - 120 of 363 results
  1. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...
  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially available...reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that...transcription. This is then amplified by PCR using Taq Polymerase and then transcribed to provide RNA substrates...instead of RPA. Unlike RPA, this technique uses Taq polymerase which is widely available and less expensive....
  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...developed to quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts... transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence...effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent aptamers Corn...PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore...
  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... biology and biotechnology purposes.  Once a polypeptide is biotinylated (more on this below) it can be...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated ...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...
  5. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...single stranded regions with homology to anneal a polymerase, to fill in the gaps a DNA ligase, to seal the...than 200 nucleotides before the annealing and polymerization steps could occur. Secondly, it does not work...instead of using the exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or ...methods that have been described--CPEC (circular polymerase extension cloning) and SLiCE (Seamless Ligation...
  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters... sensor, membrane depolarization increases the BRET signal whereas hyperpolarization decreases the signal...
  7. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are...Na+) against the concentration gradient and hyperpolarize the cell (Mattis et al., 2012). Bicistronic ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they... A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES is an optogenetic tool developed for bidirectional...
  8. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...join any fragments end-to-end without the exo/polymerase generation of annealed complementary regions....and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping enzyme superfamily of...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....
  9. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...binding to the template strand (strand used by RNA polymerase II for transcription) are more effective for ...editing! Pooran Dewari is a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand...
  10. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    ...Updated March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name!...participation in suggesting names and voting in our poll. Now, we'd like you to meet..... *drumroll* Aavery... out this form here linked below. We’ll keep the poll open until February 28 5PM EST and we’re excited...
  11. Control AAV Preps

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    Collection
    ...Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Feng 27056...
  12. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... uses do you foresee for this tool? Howarth: Polyproteins are useful for mechanical study of proteins ...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell ...References: SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture...
  13. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...microelectronic fibers made from thermoplastic polymers that can work with all the bells and whistles....called thermal drawing, essentially where blocks of polymers are heated up and pulled to a desired thickness... small molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     ...
  14. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...
  15. TALEN Plasmids and Kits

    Type
    Collection
    ...specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View...
  16. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... the reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew...expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction...Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020...
  17. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    .... The topic of unionization continues to be a polarizing one. High-profile universities remain outspoken... panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office Ben Corb - Director... in the intersection between science and public policy. Follow David on Twitter @driglar.   References...
  18. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...advantage of the program. Start the program by polling the audience with a few questions. Who is out there...in response. I am always ready with some written poll questions to get things started. Give a couple of...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect...
Showing: 101 - 120 of 363 results