We narrowed to 119 results for: SAG

...results in the pooled library lentiviral prep. B) Usage of a pooled library lentiviral prep for experiments...

...each lot of AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’...

... online video market ever since. The take home message of all of this appears to be that video is the ...

...protein folding, and deal with sub-optimal codon usage (Table 1) A few mutations are common to all or most...

... to a chalk talk is to effectively convey your message. Key Things to Think about When Developing your...

...slides and how the slides complement the spoken message. Learn from their level of explanation in presenting...

... larger cells. Note, we don’t mind the warning message at the top, as our cells of interest do not lie...

...achieve success in their careers. The take-away message I took from the career panel was that there are...

...expression of that gene in cells that also express GAL4. Usage of this system revolutionized genetic studies in...

...clones were bi-allelic in our hands). Take-home message In our hands, delivering Cas9 protein in complex...

...modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max with a 4.2-6-...

...valproic acid in 200 mL deionized water Sterilize by passage through a sterile 0.22 µm filter. Prepare 10 mL...rpm. Pro-Tip Do not use cells that are over 30 passages. Section 2: Transfection Check the cell density...

...your cells. Thaw a new vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media...

...antibiotics to the media. Use cells that are below passage 20 for viral production. The optimal mass DNA:mass...

...pen-strep to the media. Use cells that are below passage 15 for viral production. Procedure Seed 293T packaging...

...your cells. Thaw a new vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media...

...your cells. Thaw a new vial of cells after 20-30 passages. Do not add penicillin/streptomycin to the media...

...transfection. Thaw a new vial of cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T...

... overnight. Although cells should regularly be passaged in DMEM + 10% FBS with penicillin/streptomycin...