We narrowed to 237 results for: SPL;

...popular tools for studying frogs include egg transplants and microinjections. Injections of mRNAs, for...disease-relevant genes in such a controlled environment displays how essential the frog is as a biomedical model...

...authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity...from Twitch-2B (Matlashov et al. 2022). iGECInano displays better response kinetics and photochemical characteristics...

...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., ...and higher contrast than previously developed NIR split probes (Tchekanda et al., 2014, Filonov et al., ...

...to a number of rare genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital...Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. ...

... effective in both NHPs and mice, but they can display species-specific tropism. For example, AAV9-X1.1...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive..., lung, and thymus, and transduce the heart and spleen at similar efficacy levels to AAV9 (Stanton et ...

...versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments...biocytin-filled cell expressing BiPVe3-dTomato, displaying characteristic PV basket cell morphology. Adapted...

...viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue or orange-blue rather ...>Replace Red with Magenta. Or you can use Color>Split Channels and then Color>Merge Channels into whatever...

...tend to be choc-full of open reading frames that display little to no homology to anything else, making ...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...

...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic.... Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing ...

...LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial ...Nevertheless, amiRNA-based RNAi experiments routinely display highly specific and robust gene silencing (following...

...prevent off-target expression of the sensitive FLEx-split TVA receptor used for tracing neural pathways was... with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and Cell Tropism after ...

...emission maximum. Note, you can also use the tool to display excitation spectra, which I excluded here for better...created through the actual APC-Cy7 fluorophore but displays an emission spectrum that overlaps with APC-Cy7...

...because the top figure shows the construct from our 'Split RNA' design, where the pre-processing form of crRNA... more details. One important note is that this ‘split RNA’ design is not as efficient in genome editing...

...Rajat Rohatgi 107855 pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G) TARDBP CMV ALS Rajat...Rajat Rohatgi 107860 pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV) TARDBP CMV ALS Rajat... VWM disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD Stefan Stamm...Bernhard Lechtenberg 169375 pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd...

...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive...

...membrane and gel during the transfer, while dark splotches could indicate dirty equipment, degraded or improperly...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

...cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA Helper virus for monosynaptic tracing...

...station if unwanted or biohazardous materials are splashed onto your face and/or get into your eyes. Wash...shaking or mixing, which may produce aerosols or splashes. These procedures should be done underneath a ...

...improved peroxidase reporter, APEX2, through yeast display. APEX2 has been shown to exhibit superior performance...functional complementation between two non-fluorescent split GFP fragments. When the two fragments, expressed...

...region is removed from the mRNA due to alternative splicing, and an early frameshift mutation is more likely...