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We narrowed to 136 results for: THY-2

Showing: 101 - 120 of 136 results
  1. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew...
  2. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for...overproduces hydrosulfide ions yes - Grow with methylmercury. S. cerevisiae  no no Can be used for color ...
  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover...
  4. All in a Twist: dsRNA

    Type
    Blog Post
    ... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...introduce some of its dsRNA intermediates into a healthy individual to provoke an immune response and then...
  5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...excise the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al...and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually...rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected with HIV-1....
  6. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...
  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the interaction between the sender cell...targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these...
  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., &...
  9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...
  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...are detargeted from the liver, kidney, lung, and thymus, and transduce the heart and spleen at similar ....org/10.1038/s41592-020-0799-7 Lawler, A. J., Ramamurthy, E., Brown, A. R., Shin, N., Kim, Y., Toong, ...
  11. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...efficient rate. These cuts most often occur in thymine-rich regions on the non-target strand. Occasional...
  12. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise... for the regulatory services department as our healthy homes program was moved from one department to ...
  13. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...producing lentivirus in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching...
  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...430825, 175 cm 2 Cellstack 5, Corning 3319, 3180 cm 2 Cellstack 2, Corning 3269, 1272 cm 2 Heat-inactivated...: 50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl 2 Add the following to the 2 L sterile bottle: 1836 mL deionized...up to 2 months. After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE GLYCOL... mL of PBS. Aspirate PBS and add 2 mL of 0.05% Trypsin/EDTA. Wait ~2 min. Neutralize trypsin by adding...40% Polyethylene Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl2...Cell-Stack (CS5) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-175 flasks). Cell...flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells Day...
  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward...or too old. Ensuring that you are working with healthy, happy cells is one of the easiest ways to improve...
  16. General Transfection

    Type
    Protocol
    ... solution can be stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working... transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high...aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear MW 25,000 Da (PEI) Dissolve... for viral production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace...Biological Safety Cabinet 0.5–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel... 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...times a week: Monday: Plate 1x10 6 cells in a 75 cm 2 flask in a volume of 15 mL. Wednesday: Plate 1x10 ...
Showing: 101 - 120 of 136 results