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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 139 results for: THY-2

Showing: 101 - 120 of 139 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... a template, copying the base edit. Figure 2: Cytidine deamination takes place on the free strand...SpCas9 base editors with editing windows as small as 1-2 nucleotides. To reduce off-target effects associated...formation. To further decrease indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to ... DNA. Uracil is then subsequently converted to thymine through DNA replication or repair. Fusing an inhibitor...

  2. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland Clinic. Gram Stain. https...iodine solution. Rinse briefly with tap water. Add ethyl alcohol or acetone drop by drop onto the smear until...

  3. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547 Kunda, Z. (...were much more likely to rate that study as untrustworthy, inaccurate, methodologically flawed, and biased...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...therapies produce lasting side effects, damage healthy cells, and often cannot eradicate metastasis or...

  5. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew...

  7. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for...overproduces hydrosulfide ions yes - Grow with methylmercury. S. cerevisiae  no no Can be used for color ...

  8. All in a Twist: dsRNA

    Type
    Blog Post
    ... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...introduce some of its dsRNA intermediates into a healthy individual to provoke an immune response and then...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover...

  10. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...excise the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al...and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually...rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected with HIV-1....

  11. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the interaction between the sender cell...targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these...

  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., &...

  16. CRISPR Guide

    Type
    Guide
    ...left free to interact with the target DNA. Figure 2: Overview of the NHEJ repair mechanism Cas9 will only...systems enable researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single...Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...your experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with...the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and Cas12...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...off-target effects by using a single Cas9 nickase and 2 different gRNAs, which bind in close proximity on ...

  17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...are detargeted from the liver, kidney, lung, and thymus, and transduce the heart and spleen at similar ....org/10.1038/s41592-020-0799-7 Lawler, A. J., Ramamurthy, E., Brown, A. R., Shin, N., Kim, Y., Toong, ...

  20. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...efficient rate. These cuts most often occur in thymine-rich regions on the non-target strand. Occasional...
Showing: 101 - 120 of 139 results