We narrowed to 136 results for: THY-2

...JS, Legube G, Miller KM Genes Dev. 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus...gene a megabase away, or looking at the histone methylation status at a double-strand break that needs repairing...

... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew...

...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for...overproduces hydrosulfide ions yes - Grow with methylmercury. S. cerevisiae  no no Can be used for color ...

...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover...

... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...introduce some of its dsRNA intermediates into a healthy individual to provoke an immune response and then...

...excise the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al...and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually...rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected with HIV-1....

... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...

...neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the interaction between the sender cell...targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these...

...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., &...

... tabular HTML formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow...Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity...database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope, curated content, and ease...

...out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are...of many posts in our career series.  “The only healthy communication style is assertive communication....

...split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin... Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools: Split-TurboID for protein...

...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...are detargeted from the liver, kidney, lung, and thymus, and transduce the heart and spleen at similar ....org/10.1038/s41592-020-0799-7 Lawler, A. J., Ramamurthy, E., Brown, A. R., Shin, N., Kim, Y., Toong, ...

...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...efficient rate. These cuts most often occur in thymine-rich regions on the non-target strand. Occasional...

...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise... for the regulatory services department as our healthy homes program was moved from one department to ...

...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...producing lentivirus in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching...

...430825, 175 cm 2 Cellstack 5, Corning 3319, 3180 cm 2 Cellstack 2, Corning 3269, 1272 cm 2 Heat-inactivated...: 50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl 2 Add the following to the 2 L sterile bottle: 1836 mL deionized...up to 2 months. After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE GLYCOL... mL of PBS. Aspirate PBS and add 2 mL of 0.05% Trypsin/EDTA. Wait ~2 min. Neutralize trypsin by adding...40% Polyethylene Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl2...Cell-Stack (CS5) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-175 flasks). Cell...flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells Day...

...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward...or too old. Ensuring that you are working with healthy, happy cells is one of the easiest ways to improve...

... solution can be stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working... transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high...aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear MW 25,000 Da (PEI) Dissolve... for viral production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace...Biological Safety Cabinet 0.5–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel... 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...times a week: Monday: Plate 1x10 6 cells in a 75 cm 2 flask in a volume of 15 mL. Wednesday: Plate 1x10 ...