...Bacterial Transformation Summary The following technique can be used to easily move any piece of DNA from... by restriction digest is a commonly used lab technique. For the purposes of this tutorial we will discuss...from one plasmid to another. However, the same technique can be used to move promoters, selectable markers...

...for Brain Science BRAIN Armamentarium Collection Caltech Systemic Capsids AAV Packaged on Request AAV Guide...

...for a stock mixture is an essential laboratory technique, as imprecise measurements can affect the outcome...weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the materials...

...insert and vector backbone are known and thus this technique can be quickly used to verify your plasmid. The... should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid fingerprinting...

...Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the biosafety...higher speeds and are used for more specialized techniques. Regardless of the type of centrifuge you use...

...ET SSB) to the isothermal reaction mix. ET SSB protects 3’ ssDNA ends from the ssDNA-specific endonuclease... is to “stitch” together several oligos. This technique is especially useful for introducing promoters...

...advantage of the physical properties of light to detect small objects. Two of the most important properties...Procedure Set up your microscope by removing any protective cover that may be on it and plugging in the power...

...EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when working with this chemical. Note... damage to the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield...

...the literature for an appropriate sterilization technique if you are working with any weird and wonderful... into your ~60 ℃ molten gel mix using sterile technique. Swirl the agar bottle to ensure even distribution...

...GAGTGGGAGGGTCCCGTCCT 68060 cut S. pyogenes Fungal Biology and Biotechnology 2015, 2:4 Hong Ctnnb1 M. musculus AGCTCCTTCCCTGAGTGGCA...

...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...

... able to distribute materials containing FLEx technology. Read the genOway press release for more information...

...preparing and handling lentiviral particles. Personal protective clothing should be worn at all times. Use plastic...gene delivery in vivo. Zufferey R et. al. Nat Biotechnol . 1997. 15(9):871-5. PubMed . Self-inactivating...

...do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start by streaking...

...MCS of an empty vector. However, the following technique can be used just as easily to add short tags or...

...digestion is commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also...

...Bacterial Culture Introduction Many molecular biology techniques require highly purified and concentrated plasmid...

...between the oligo primers. This molecular biology technique creates several micrograms of target DNA from ...

... is toxic. When handling, use proper personal protective equipment including laboratory coat, laboratory...

...meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (ideally...