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We narrowed to 797 results for: Tec

Showing: 101 - 120 of 797 results

  1. Pouring LB Agar Plates

    Type
    Protocol
    ...the literature for an appropriate sterilization technique if you are working with any weird and wonderful... into your ~60 ℃ molten gel mix using sterile technique. Swirl the agar bottle to ensure even distribution...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...preparing and handling lentiviral particles. Personal protective clothing should be worn at all times. Use plastic...gene delivery in vivo. Zufferey R et. al. Nat Biotechnol . 1997. 15(9):871-5. PubMed . Self-inactivating...

  3. Protocol - How to Streak a Plate

    Type
    Protocol
    ...do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start by streaking...

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    ...meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (ideally...

  10. CRISPR Library Amplification

    Type
    Protocol
    ...to lysis by freeze-thaw if not suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the...

  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...They also have a limit of detection of ~5%. This means they do not reliably detect editing events that occur...primary methods of off-target detection: biased and unbiased. Biased techniques only sequence certain sites...: Biased Genotyping Options Technique Sequence Information Detection Limit Advantages Disadvantages...highly sensitive with detection levels as low as .01% (Hendel et al. 2015). Low detection rates mean the investigator...off-target mutations if they remain undetected using these techniques. Frequencies of off-target mutations...have a minimum detection limit of 0.1% (Tsai et al. 2015). This contrasts with detection frequencies of... Table 2: Unbiased Genotyping Options Technique Detection Limit Application Advantages Disadvantages...

  12. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... for detection RANbody is detected enzymatically with HRP substrate More sensitive than detecting antigen...require a secondary antibody for detection. Instead they are detected with tyramide signal amplification...and is useful for detecting GFP. Spaghetti Monster tagged RANbodies can be detected either with a labeled...sensitive than HRP RANbody detection, it is approximately as sensitive as detecting the same antigen with ...RANbodies are easily detected with a labeled anti-chicken antibody. Chicken IgY RANbody detection is less sensitive...sensitive than HRP RANbody detection but approximately as sensitive as antigen detection with a standard primary... When detected with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting antigen...

  13. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...different biotech companies which are enzyme-based mycoplasma detection kits. These kits detect mycoplasma...irreplaceable or highly expensive). Detecting mycoplasma contamination Detection of mycoplasma by naked eyes ...Another method used for mycoplasma detection is using PCR. In this technique, PCR primers specific to the 16s...cells that can detect the presence of mycoplasmas in the culture. Once these cells detect mycoplasmas, ...Assistant at MGMs Institute of Biosciences and Technology. If you are reading this article then you probably...through cross-contamination and due to poor lab techniques. These include reusing pipettes for multiple ...turbid nature, contamination is difficult to be detected in the serum that is most commonly used for every...

  14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...that require scientists to detect proteins in their samples. One technique that relies heavily on antibodies...Enzyme-linked Immunosorbent Assay. ELISA are used to detect proteins within a 96- or 384-well, flat-bottomed...bottom of the well), and then its concentration is detected through visualization of an enzymatic reaction...ELISAs, which differ in the way the antigen is detected. These are sandwich, competitive, direct, and ...available ELISA kits are for a sandwich ELISA. This technique works by first coating the wells of a flat bottomed...specific to the protein you are interested in detecting in your sample. When an ELISA kit is ordered, ... your protein of interest.  The next step is to detect the protein-antibody pair. In a sandwich ELISA,...

  15. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...Addgene.org Addgene Help Center: Tech Transfer Technology Transfer Information Tech Transfer News: A Reintroduction...get an Addgene account for Tech Transfer Offices. They’re packed with tech transfer resources (summaries... question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...stats and strategies with tech transfer offices. Drop us a line at techtransfer@addgene.org. Albert Einstein...’re trying to make MTA turnaround faster with technology transfer offices (TTOs). The latest of these ...deliver the very first ones in person by our own Tech Transfer Team! Why MTAs Matter Though they may seem... important—they guarantee certain rights and protections for depositor and requestor alike. That’s why...

  16. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications...development of new biological technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP... to develop new technologies. Majority of them are eager to just "import" technologies developed abroad...The Fujii lab specializes in developing novel technologies to analyze molecular mechanisms of genome functions...extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In the last several...years, my lab has been working on development of technologies for biochemical analysis of genome functions... chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity purification...

  17. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...cannot imagine any use for GM technology other than to sustain those techniques. We feel strongly that the...transformation technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full...there are a lot of unexplored areas in plant biotechnology that are ripe with opportunity. We’ve decided...One of the biggest difficulties that plant biotechnologists face is public perception of genetic modification... crops has burdened this emerging engineering technology. Monoculture and chemical spraying, mainstays... current benefits and future potential of the technology to the public in an open, responsible manner....this challenge directly by developing a plant biotechnology product specifically for consumer sales. Flowers...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...genetically encoded photocaging technique to insert a light-removable protecting group, specifically a nitrobenzyl...have been making major improvements to CRISPR technology since its initial applications for genome engineering...prime editing, anti-CRISPR proteins, and other techniques all aim to improve targeting specificity or reduce...2015, scientists combined CRISPR and optogenetic techniques to develop a variety of photoactivatable CRISPR...spatially control gene expression. Both of these techniques take advantage of light-induced dimerization ... their CRISPR system. This common optogenetic technique can be employed in other split systems where light...activated with light and lowered background DSBs (non detectable). Like their prior work, the Sato lab also showed...

  19. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...LDR can reliably detect single-base differences. The original creators of this technique visualized the ...Development of a ligase detection reaction/CGE method using a LIF dual‐channel detection system for direct ...-based ligase detection reaction assay using a molecular beacon probe for the detection of low-abundance...other thermostable DNA ligases. These applications detect specific nucleotide sequences with high specificity... (SNPs) (5,12,13). For example, in the Ligase Detection Reaction (LDR), probes complementary to the sequence...methods to greatly increase the sensitivity of detection (14-20). LDR has also been extended to multiplexed...multiple potential SNP sites (15). The related LCR technique uses two sets of probes to achieve exponential...

  20. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...labeling strategy to detect cell-cell interactions in vivo (Ge et al., 2019). This technique relies on an S.... with biotin using avidin coated purification techniques. Due to the high affinity of biotin for avidin...proteins The first biotin based proximity labelling technique, BioID, was developed in 2012 in the Brian Burke... developed as well as new proximity labelling techniques. These newer tools are also currently available...combines a protein fragment complementation (PCA) technique with proximity labelling to validate binary protein-protein...proteins in one simple experiment. PCA is an in vivo technique where two proteins are fused to complementary ...Split-BioID blog post.  RaPID: RNA-protein interaction detection So far, we’ve covered ways to study protein-protein...
Showing: 101 - 120 of 797 results