Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 958 results for: Tes

Showing: 101 - 120 of 958 results

  1. Antibody Plasmid Collection

    Type
    Collection
    ...Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies made from...

  2. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...

  3. AAVED

    Type
    Collection
    ... give a 20 minute presentation, followed by 20 minutes of questions/discussion. The goal of the presentation...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ...number of plates you’d like to pour. For example: Because we’d like to make 20 plates, and our plates can hold... and negative test results. Sample Data In all cases below (-) indicates that the tested strain is not... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may...Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10...colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of ...appropriate number of plates and stack them on your lab bench. Label the plates with the date and the ...remainder of the plates, pour directly from the bottle. Pro-Tips Be sure to swirl your plates after pouring...

  6. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences...

  7. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, ...neutralized. The B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization...cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission...happening.”  In their assay, the neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses...

  8. CRISPR Plasmids - Plants

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

  9. Open Enzyme Collection

    Type
    Collection
    ...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

  10. Ras Pathway

    Type
    Collection
    ...colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251...

  11. RNA Extraction Without A Kit

    Type
    Blog Post
    ...your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent ...

  13. DNA Quantification

    Type
    Protocol
    ...readings than a DNA sample dissolved in buffer (such as TE). You will get much more accurate and consistent ...ranges from 1.80-2.00). Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...have been used to visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons ... The central nervous system (CNS) orchestrates complex processes enabling organisms to control their ... affects the labeling of small structures like neurites. Like epitope tags, low FP expression may be insufficient...structures through their multiple, high-affinity binding sites for primary antibodies which can be used to amplify...difficult to label. It has also been shown that dendrites in the stratum oriens and the stratum lacunosum...their multiple, highly specific antibody binding sites and primary antibody-based detection, smFPs create...

  16. Ligation Independent Cloning

    Type
    Protocol
    ...digestion and PCR products with sterile water (instead of TE buffer) to ensure optimal salt concentrations in ...room temperature for 30 minutes. Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent ... for 5 minutes at room temperature, then add 1 μl of 25 mM EDTA, followed by another 5 minutes at room...Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-consuming ligation step ...with a single enzyme, and remove the restriction sites from the final product (no "cloning scars"). A “...reaction may be stopped by heating to 75° for 20 minutes. Step 6: Anneal and Transform Mix your treated ...

  17. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    .... https://doi.org/10.1038/s41592-019-0614-5 Sztal TE, Stainier DYR (2020) Transcriptional adaptation: ...inverted repeats separated by a loop region that facilitates the formation of a hairpin RNA structure, resulting... gene of interest. Tools for RNAi Arguably the greatest advantage of RNAi is the straightforward nature...active development, the first step necessarily dictates the direction and scope of the subsequent two....Artificial micro RNA and short hairpin RNA The greatest limitations of the long hpRNA approach are thought...experiments typically involved antibiotic selection to test for the presence of the RNAi transgene, followed...Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, ...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...mouse)/R. norvegicus (rat) rat IgG2a Addgene distributes ready-to-use recombinant antibodies made from...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...attB sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...reactions. Recombination of attP and attB sites creates attL and attR sites.   Gateway vectors contain modified...takes place between the attB sites flanking the insert and the attP sites of the donor vector. This reaction...enzyme mix and generates the entry clone containing the DNA of interest flanked by attL sites. As a byproduct...reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression clone ... place between the attL sites of the generated entry clone and the attR sites of the destination vector...attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with ...
Showing: 101 - 120 of 958 results