...viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. Do you have suggestions...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter...

...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...

...control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ...

...all cells and all tissues. Ras can activate a variety of pathways, which ultimately turn on genes involved...

...Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein RBD mutations for comprehensive mapping...

...) (Link opens in a new window) is a library of various python functions for the purpose of designing and...

...Peterson, and Yeh labs at MGH to target sites within various endogenous zebrafish genes; many of these were ...

...vector DNA and insert DNA used in the ligation will vary depending on the size of each and their concentration...failed ligations. The following table indicates the various controls: Control Ligase Interpretation Uncut vector...

...D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham N, Cristiano C, Dorrier CE, Tipton GJ...

...recombinant antibody can be purified for use in a variety of applications. Sharing speeds science. We believe...to mix. Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should be empirically determined...

...Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, ...

...the clones to incubate at 37 °C for 7 - 14 days. Vary these times depending on the doubling time of the...

...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...protocol. Sample Data Figure 1: HEK293T cells at various confluencies. This AAV production protocol should...

...Lentiviral expression of firefly luciferase Paul Khavari 109395 EFS-Vector Firefly EF1α AAV-CRISPR knockout...

... (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635 NS3 C 79637 NS2B C 79636... activity have remained challenging due to the variable availability of enhancer sequences in different...the entire genome for genes that regulate a wide variety of phenotypes, including but not limited to cell...CIDAR MoClo) Parts Kit. This Kit consists of a variety of plasmids containing many different promoters...RBS’, coding sequences (CDS), and terminators of varying strengths. These can be easily combined with your...creation of many E. coli constructs with greatly varied levels of expression. The 93 plasmids in the CIDAR...gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts...

...experiment would need to be repeated to account for variability....

...in your protocol. How to set the temperature will vary depending on the model of water bath, but will likely...

...recognize a large range of DNA sequences. Given the variety of these enzymes and the unique sites they recognize...