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We narrowed to 397 results for: abo.4

Showing: 101 - 120 of 397 results

  1. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...adaptive immune responses, which flies do not have!) (4). The power of Drosophila as a model organism is in...PMID: 22038952. PubMed Central PMCID: PMC3677821. 4. J. R. Huh et al. Digoxin and its derivatives suppress...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome Engineering...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post
    ... mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes, such... toxicity in long-term experiments.  Figure 4: The C-terminus of Cre (CreC) and the N-terminus of... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

  3. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...and 70186, respectively). Antares Chu, J., et al. (4), likewise, wanted to produce a BRET probe for imaging...modular base-specific gene tagging using a ligase-4-dependent mechanism. Nature Comm. 7, 12338. PubMed...PMID: 26424696. PubMed Central PMCID: PMC4668208. 4. Chu, J., et al. (2016) A bright cyan-excitable orange...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often... emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

  4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post
    ...tubes, and would prevent the pH change upon thaw (Fig.4). To test this, we froze the tubes at -80°C and then...transport or storage on dry ice. Nat Methods. 2013 Apr;10(4):278-9. PubMed PMID: 23538862. 2. Potter M, Lins B...return the sample back to the original pH. What about putting it back in the freezer? We then tested whether...

  5. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were first... Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni,... Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic...

  6. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...expectations. (Don't think you can finish that book in 4 weeks!)” “Sometimes a mentor is a sounding board--...Connie Cepko to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this... answers to the top questions scientists ask me about mentoring. Here they are: What makes a good Mentor... Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do I ask someone...a good science mentor? I started a conversation about many of these topics with the researchers who joined...often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela Depace's...questions in future posts. If you have a question about mentoring that you want us to cover, let us know...

  7. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Cell Stem Cell 4, no. 6 (2009): 472-76. PubMed PMID:19481515. PubMed Central PMC: 2705327  4. Hockemeyer,...reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. PubMed PMID: 21339765. Methods figures...PubMed PMID: 20139967. PubMed Central PMCID: 2892897. 4. Stadtfeld, M., and K. Hochedlinger. "Induced pluripotency...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check...

  8. The Future of Research Symposium Boston 2015

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    Blog Post
    ...futures both inside and outside of academic research (4). There are currently 17 sites around the USA undertaking...PMID: 22567149. Pubmed Central PMCID: PMC3342243. 4. Mathur, Ambika, et al. "Transforming training to ... the Jackson Laboratory for Genomic Medicine and a Senior Research Associate at the Labor and Worklife...increasing data collection about the scientific workforce, in particular about postdocs. Among other involvements...Research Symposium Boston 2015 – Academic Data and the Labor Market. For more information on the symposium as...generating too many trainees per PI” “Lack of awareness about how the system operates and functions” “Complete...available in academia (5, 6). Academic data and the labor market for PhDs Later this week, postdocs and students...

  9. RNA Extraction Without A Kit

    Type
    Blog Post
    ...Alternatively, glassware can be baked at 180°C for at least 4 hours.   If your final RNA sample(s) are resuspended...time it took to isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still...more foolproof (still without a kit!) As mentioned above, working with RNA requires keeping your samples ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...supernatant is extracted with chloroform as in the above no-kit method.  Researchers have also developed ...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    ...carbohydrates added to antibodies post-translationally. 4) Modifications made to antibodies, such as inclusion...Heterogeneous Populations to Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390/antib4030197  Hegazy...Check out our post on Biotinylation to learn more about biotin and its use in molecular biology. Other Conjugates...perfectly blissful, productive lives without thinking about these chemical reactions. After all, there is an...ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

  11. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...DNA, then they could diminish off-target cutting (4). However, instead of weakening interaction sbetween...PMID: 25079318. PubMed Central PMCID: PMC4176945. 4. Kleinstiver, Benjamin P., et al. "High-fidelity CRISPR–Cas9...With this new found structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis...edits in mammalian cells and may decrease worries about off target effects in applied and/or therapeutic...at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1 Read How Non-Homologous End Joining...

  12. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...homozygous alleles at both of your genes of interest. About 1 in 4 mice will be homozygous for the mutation at ...mice, as well as progeny from future generations. 4. Continue breeding your colony You’ll want to generate...Find our Plasmids 101 blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts... posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide Learn more...

  13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...killer gene”. Gene. 1994 Oct 11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard, P., et al. “The F plasmid CcdB...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

  14. Enabling high school research at the Journal of Emerging Investigators

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    Blog Post
    ...Encouraging students to do science anywhere.  Chapter 4 - JEI's mission and how they assess whether or not...working in a lab for the first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing...resources Use our Plasmids 101 eBook to teach students about basic plasmid biology Use or CRISPR 101 eBook to...

  15. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for ... and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central PMCID: PMC4666719...Additional Resources on the Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...commercial vendors. These libraries usually contain 4-12 gRNAs/gene, which results in libraries containing...research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali...PMID: 25075903. PubMed Central PMCID: PMC4486245. 4. Ma, Hongming, et al. "A CRISPR-based screen identifies... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...

  17. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...that should work well. Quickly after transfection (4-8h), cells start releasing viral particles into the...after transfection. This supernatant can be stored at 4 °C or can be directly added to your target cells. ...Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech. 4. Walter, W., and U. Stein. "Viral vectors for gene...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...even your own! You should therefore be cautious about inserting potentially harmful genes into a transfer...

  18. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post
    ... We have been using yeast models (3, 4) to answer questions about the evolution of the genes involved ...PMID: 24807111. PubMed Central PMCID: PMC4012490. 4. Hoffman, C.S., Wood, V., & Fantes, P.A. An ancient...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques...species, but these studies generally leave questions about gene function wide open. What if two seemingly similar...question, the plasmids are also used to teach students about restriction mapping and western blotting. Students...communicating science to the public. You can find out more about Clare at the Biology Dept. site.  References 1. ...PMC4596657. 5. Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae...

  19. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate...system a promoter controls the expression of a GAL-4 driver that can bind to activation sequences upstream...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids...always emphasized the need for open science and collaboration. The field already has comprehensive reference...planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check out their...elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative polyadenylation...addition to the wealth of open resources described above, there were many plasmid tools presented at the ...

  20. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post
    ...are often looking for outreach volunteers as well. 4) Blog - Hone your writing skills Take the DIY approach... graduate course, UWE Bristol offers an intensive 4-day Masterclass in science communication. The National...Sainsbury Laboratory. She is passionate about science communications and loves talking about science, ...Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early career researcher...break into science communication? Everybody goes about this in their own way. The career paths into science...Society gives training in media skills, writing about your research and residential communication. If ...
Showing: 101 - 120 of 397 results