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We narrowed to 165 results for: cas9 expression vector

Showing: 101 - 120 of 165 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    ...fluorescently labeled. Using lentiviral vectors to stably or inducibly express tagged iPAK4 from different promoters...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in vitro applications...activation events as bands of induced GFP-iPAK4 expressed under promoters like the tet-responsive element...to create a three-dimensional atlas of protein expression in the adult mouse brain using thick-section ...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    .... The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with Cas9 in a wide...information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for cancer modeling... (20bp). CRISPR-Cas9 Cre expression vectors for genome editing in the brain Using Cas9 mice Platt et al...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system ...formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and...2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human ...

  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR...all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase...cassettes are assembled into a Cas9 expressing vector. Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency...single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs...in which Cas9 has been integrated into the genome. gRNA Empty Vectors Many gRNA empty vectors have been... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate...meningitidis Joung Cas9 sgRNA vector 68463 Mammalian U6 none S. pyogenes Zhang Cas9/pTREX-n 68708 Other...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) CRISPR...cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA guide is typically...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...Ma X, Gao F, Guo Y. Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar ...specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few spCas9s which have been evolved...of Maya Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization...

  5. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) ...Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance...Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with... and PE5 prime editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves...

  6. Plant Plasmids and Resources

    Type
    Collection
    ...creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing...intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series of vectors and DNA modules ...full selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. ...CRISPR/Cas9 Toolkit for use in a variety of plant species (e.g. maize and Arabidopsis) and vectors for easy... assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector... GreenGate Cloning System to create plant expression vectors containing several cassettes and generate...

  7. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG)...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid... none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  8. Plan Your Experiment

    Type
    Collection
    .../or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and ...Figure 1). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...

  9. Lentiviral Prep Service

    Type
    Collection
    ... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to...4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG)...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin..., need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (...

  11. Worm Expression Resources

    Type
    Collection
    ... genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous... the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...

  12. Lentivirus Production

    Type
    Protocol
    ...Lentivirus Production You may also like... Viral Vector Guides Mol Bio Protocols Introduction This protocol...be used to produce lentivirus from a lentiviral vector transfected into 293T cells using a polyethylenimine...Figure 1: 293T cells were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...

  13. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...transgene. Typically, the desired target of RNAi is expressed as inverted repeats separated by a loop region...considerations, such as the starting material (DNA-based vector or purified RNA) and the initial state of the dsRNA... easiest approach to induce RNAi involves the expression of long hairpin RNA (hpRNA). This method is facilitated...construct has unique features, such as inducible expression of the hpRNA and/or fusing your gene target sequence... Although downstream work is required, several vectors can be found at Addgene that could serve as an ...an excellent start (#22988, #137884). Both vectors allow the creation of amiRNAs based upon miRNA precursors...Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...

  14. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG)...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...designed for use in Xenopus. Cut Fully functional Cas9 enzymes designed to introduce a double-strand break...break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of...Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas...RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...

  16. CRISPR References and Information

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    Collection
    ...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF,... gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, ...gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... Injection and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122...

  17. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG)...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid... Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG)...none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...

  19. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...recombination, RFP is expressed. Cre can mediate one of two deletions to allow YFP or M-CFP expression; these deletions...ways to spatially and temporally control gene expression, especially in in vivo models, and new Cre-lox...fluorescent protein in the construct, OFP (orange), is expressed when Cre is absent; stochastic Cre excision results... and inversion. The Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) ...remaining cassette can invert as long as Cre remains expressed; once Cre is removed from the system, the fluorescent...coding sequence is closest to the promoter will be expressed. This system differs from Brainbow-1.0 in that...

  20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the key tools now providing access...continues to be challenging. Organism specific viral vector systems would also be beneficial for many systems...snail can be manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated...-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the arthropod germline by injection into ...
Showing: 101 - 120 of 165 results