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  2. RUBY-Red Siliques

    Type
    Blog Post
    ... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ...

  3. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ..., GPI, and SGZ, to improve neuronal membrane trafficking and localization. Improved photophysical properties...al…, Supplementary Figure 2.   Improved trafficking and localization through different C-terminal...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse range of... molecules. Other members’ projects range from hacking probiotics to filming educational lab videos, while...

  6. The MTA at Addgene

    Type
    Blog Post
    ...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ...

  7. How to Lead a Great Meeting

    Type
    Blog Post
    ...Accountability: At your next meeting, start by checking in on the action items from last meeting. Use ... Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside Addgene Posts...

  8. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...will define positive selection as the means of picking up positive traits (i.e. the plasmid contains a...toxin), whereas negative selection would be the picking up of a negative trait (i.e. the plasmid contains...

  9. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...— by directly typing terms in a search bar, by ticking some boxes, by selecting a suggestion — how should...should these be supported and combined?  Luckily, we had the advantage of a very specialized domain of ...

  12. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...and so was really starting from the beginning. Luckily, a fellow graduate student and a data analyst in...comment in C++ and Java Command line history: Tracking command line history by recording all commands...

  13. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...HA staining is weaker than reporter staining. Picking the right reporter for your RANBody While all three... with these plasmids from Penn State Need help picking the right fluorescence microscopy technique to ...

  15. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Hegazy, et al., 2020; Niemeyer, et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for ...–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...Epigenetic engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron...human body and G-baToN provides versibility in tracking and modulating the interactions between cells....

  17. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...tweet in order to easily access it later. Pro tip Checking the number of retweets, likes, and comments on...altmetric score, is also displayed in your feed. By clicking on a paper in your feed you can see suggested ...

  18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...manipulate full genes and observe the effects. Luckily for us, CRISPR is an incredibly versatile system...et al. 2014).  Simple knock-out experiments are trickier when it comes to long non-coding RNAs (lncRNA)...

  19. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction digest...design. Note that you can browse all components by clicking the blue text in the upper right-hand corner.  ...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...
Showing: 101 - 120 of 330 results