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We narrowed to 494 results for: control

Showing: 101 - 120 of 494 results

  1. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...include controls to show that your antibodies are binding specifically. For positive controls, aim to ... and isotype controls are also commonly used in IF experiments. Precisely which controls you perform will...been manipulated to overexpress it. For negative controls, use samples in which the target is known to be...research antibodies, protocols, and appropriate controls and you will be well on your way to performing...

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...101 eBook! Researchers use various techniques to control protein expression for experimental, biotechnological...successful. Some mammalian systems also allow for control over when a protein is expressed through the use...Protein expression from each of these vectors is controlled by a different promoter resulting in different...to E. coli. Of all the vectors, pET, under the control of the T7 lac promoter and induced by lactose, ...researchers often place recombinant proteins under the control of the galactose inducible promoter (GAL). Other...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...stimulation itself, the output of the laser must be controlled. As discussed above, each opsin variant has slightly...the A.M.P.I Master-9 Pulse Stimulator, which can control output of several lasers simultaneously, can be...powerful alternatives to the Master-9 that only control one or two lasers are also available. Optimal stimulation...loss of laser power. More recently, wirelessly-controlled optogenetic implants have been reported by several...References 1. Gutierrez DV, et al. Optogenetic control of motor coordination by Gi/o protein-coupled vertebrate...

  4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...Xin hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein...their ability to be precisely spatio-temporally controlled as a key advantage over other inducible enzyme... sugars (including lactose), and a lac operon controlled GFP. When the enzyme breaks down the HMO of interest...Photosactivatable Fluorescent Proteins Use Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent...

  5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...activity in HEK293 by reducing editing from ~30% in control samples to 0-10% in Acr samples. Acr proteins can...specific site. 2. Temporal, spatial, or conditional control of CRISPR activity Regulating Acr proteins by inducible...molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas activity. For example, fusing AcrA4II...LOV2 domain allows anti-CRISPR activity to be controlled by blue light (Bubeck et al., 2018). 3. Reduce...Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org Find CRISPR...

  8. Antibodies

    Type
    Collection
    ...application-specific validation and consistent quality control measures. We work with trusted partners and experts... by sharing our protocols and quality control methods. Learn More Guide New to using antibodies...

  9. Antibodies 101: Isotypes

    Type
    Blog Post
    ...you can select an appropriate isotype control. An isotype control has the same constant heavy chain domain...doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining you see is ... than nonspecific background staining. Isotype controls are used in antibody-based applications including...

  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...antibody, you’ll want to use the same positive control across experiments to be aware of signal strength...you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing is real...real. It’s important to include a negative control using serum from the same species your polyclonal antibody...antibody was generated in or a polyclonal isotype control, to account for nonspecific binding. Now that you...

  11. Biosensor AAV Preps

    Type
    Collection
    ...proteins, including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors... 140555 pAAV-hsyn-GRAB_DA-mut Syn GRAB_DA-mut (control) none Constitutive 9 Li 140556 pAAV-hsyn-GRAB_rDA1m...140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208702 pAAV-hSyn-GRAB-rDA3m...pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE GFAP iGABASnFR2 (control) none Constitutive 1, 5 GENIE 218874 pGP-AAV-syn-iGABASnFR2...pGP-AAV-syn-iGABASnFR2(no bind)-WPRE Syn iGABASnFR2 (control) none Constitutive 1, 5 GENIE 218877 pGP-AAV-syn-flex-iGABASnFR2...-flex-iGABASnFR2(no bind)-WPRE Syn iGABASnFR2 (control) none Cre dependent 1, 5 GENIE Glutamate Sensor...Li 123310 pAAV-hSyn-GRAB_NEmut Syn GRAB_NEmut (control) none Constitutive 9, rg* Li 208687 pAAV-hsyn-NE2h...

  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...are available to confer greater transcriptional control, assist with proper protein folding, and deal with...Contains mutated lac permease whch allows for linear control of expression F- ompT lon hsdSB (rB- mB-) gal ...polymerase (RNAP) from the bacterial genome under control of the lac repressor, which is inducible by the...cases, some strains carry an additional measure of control such as the pLys plasmid, which suppresses basal...difference being that the latter provides tighter control of basal expression. What if I don't see protein...

  13. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...McClean’s lab generated an optogenetic toolkit that controls repression of gene expression in Saccharomyces...transcriptional repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders...mRNA decreased after 20 minutes in xylose (which controls expression of dCas9). The lab demonstrated the...

  15. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...basics: What is optogenetics? Optogenetics: Controlling neurons with light In 2005, a team from Stanford...1984; Harz and Hegemann, 1991). These proteins control the flow of electric charges across cell membranes...opsin genes in targeted cells makes it possible to control neuronal activity with a specificity far greater... to flow across the axon’s membrane, thereby controlling neural activity (Bernstein and Boyden, 2011; ...tool that provides precise temporal and spatial control of gene expression in vivo (Schnütgen et al., 2003...Millisecond-timescale, genetically targeted optical control of neural activity." Nature neuroscience 8.9 (2005...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    ...Tips for Plasmid Quality Control Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene Bioinformatics...Addgene, we put a lot of effort into our quality control (QC) processes to screen for and reduce these errors...non-negotiable. By maintaining a high standard of quality control, we have built a trusted plasmid catalog containing...

  17. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...sequencing results we analyze as part of our quality control process has steadily grown. On a busy week, we ... more than 200 plasmids as part of our quality control process. Consequently our team has refined our ...sequence! You might also enjoy seeing how our quality control process has changed with the introduction of next...Plasmids 101: An Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. ...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...access to transcriptional regulatory elements is controlled during development using the Drosophila wing ...the target protein. This targeted digestion is controlled by the addition of calcium, which MNase requires...only for a short period of time, thus precisely controlling the amount of cutting and mitigating noise generated...

  19. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...it is non-invasive and offers a high degree of control over time and space. Zebrafish have become a popular...had previously been a mystery, but through the control offered by the optogenetics toolbox and the versatility..., Filippo Del Bene, and Herwig Baier. "Optical control of zebrafish behavior with halorhodopsin." Proceedings...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... promoters are upstream or 5’ of the gene they control. Promoters can be organism or tissue type-specific...recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...availability of Cre, lox sites lets researchers control the spatial and temporal activation of a transgene...
Showing: 101 - 120 of 494 results