Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 168 results for: crispr library

Showing: 101 - 120 of 168 results

  1. COVID-19 Resources

    Type
    Collection
    ... plasmids Addgene's full CRISPR guide and summary of CRISPR pooled libraries . FlipGFP-based activity ...Collection page. SARS-CoV-2 Pooled Libraries - Yeast surface display libraries of Spike (S) Ectodomain and RBD... plasmid with us. Return to top Pooled Libraries Pooled Library Name Type PI Description SARS-CoV-2 Spike...Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain and...Binding Domain Deep Mutation Scanning Library Screening Jesse Bloom Library of Spike protein RBD mutations for...-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries of variant Spike protein...Mammalian Targets General Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...version,” she said. “If we learn how to make the library even better, we would be very happy to share updated...

  3. Plasmid Collections

    Type
    Collection
    ...Click here to view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections...collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics...Pathways & ORFs Synthetic Biology Pooled Libraries Pooled libraries are a set of plasmids all built with ... Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent...view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...NIH BRAIN Initiative. We also created a growing library of antibody educational resources, including our...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030... Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation...post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools...Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...Addgene.org Find all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for...base. If you heard about CRISPR, one question probably comes to mind: can CRISPR be applied to fix the defective..., Y. Multiplex CRISPR-mediated base editing in rice. In Genome Engineering: The CRISPR-Cas Revolution,...Addgene blog Read all of our CRISPR 101 blog posts Download the CRISPR 101 eBook Read our blog posts ... to facilitate repair. Since the development of CRISPR-mediated base editing (BE), these types of repairs...et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase with...adenine (A) to a guanine (G)? You may wonder if a CRISPR-base editor can perform this change. However, there...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR/Cas9 gene editing process...described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F...I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted to inactivate the gene...objective, it was surprisingly easy. It’s true, I CRISPRed. In this post, I’ll summarize the basic steps ...homologous recombination (Rago et al., 2007). Using CRISPR, I was able to knockout BRAF in a human cancer ... in Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian...maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, ...

  8. Editor's Choice, September 2016

    Type
    Blog Post
    ...Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual Plasmid...

  9. Hot Plasmids February 2024

    Type
    Blog Post
    ...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ...multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification...

  10. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...sgRNAs Generating constructs to produce sgRNA for CRISPR experiments can be laborious, requiring multiple... and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic... Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids...

  11. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...xCas9, a CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our ...our CRISPR guide Find all plasmids for base editing in our collection Sign up to get our free CRISPR eBook... et al., 2017) in combination with a synthetic library of TadA sequences to create the eighth generation...

  12. Microbiology Resources

    Type
    Collection
    ...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific...constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each of the predicted ... Check out our CRISPR reference page for CRISPR resources, such as depositor protocols and gRNA design...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression...coli strain). Addgene continues to monitor its library and distributions in accordance with export laws...

  13. Distribution to Industry

    Type
    Collection
    ...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human...Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering and MTA Information Featured...Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research ...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Nature Methods. 2015 Aug 31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos...technique, called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the...system provides users greater temporal control over CRISPR/Cas9 mediated genome modification and gene expression...139-42. doi: 10.1038/nbt.3149. Light-inducible CRISPR-Cas9 system for endogenous gene activation Optogenetics...Nucleases, the Gersbach lab has modified the RNA-guided CRISPR-Cas9 system to create a tool that is quick, versatile...robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the ...targeted to a very specific site on the genome via the CRISPR system. The ease and flexibility of the LACE technology...

  17. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase and Cre to interrogate...PI Pooled Libraries Pooled Library Name Type PI Description Mouse Tumor Suppressor Gene CRISPR Knockout...Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase (SSR) systems derived...Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Content last reviewed on...

  18. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated... CRISPR CASTs CRISPR Plasmids...Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick ...plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large...BioRender.com. Browse, sort, or search the tables below for CRISPR Transposase plasmids. Plasmids are available for...

  19. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...

  20. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian...
Showing: 101 - 120 of 168 results