Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
Type
Blog Post
...generate 2 possible products: 1) a partially dsDNA duplex with single-stranded 3’ overhangs or 2) if the... site (Figure 2, top). These primers are typically ~70 nucleotides long (20 nucleotides that anneal to... genomes.
2) Designing ssDNA oligos that avoid MMR activation: There are two ways to design oligos that...that has key MMR proteins knocked out or 2) specially design ssDNA oligos to avoid MMR:
1) E. coli with...recombineering system has three components (Figure 1): 1) Exo, 2) Beta, and 3) Gam. All three are required for recombineering...generic lambda red recombineering experiment (Figure 2). In the following sections, key steps that differ...confirmation of recombinant clones.
Figure 2: Overview of using Lambda Red recombineering system...