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We narrowed to 369 results for: epor

Showing: 101 - 120 of 369 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

  2. Science Career Options

    Type
    Blog Post
    ...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...not used for general plasmid selection. Instead, reporter genes are often employed to easily monitor transfection...expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is expressed from the plasmid...assess transfection success. GFP is often used as a reporter and we will be covering this ever-versatile fluorophore...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...usually spread unidirectionally, there are some reports of bi-directionality. For predominately anterograde...cleft is usually severely impaired, and only few reports exist about dye molecules that are transported ...2008; Watakabe et al., 2015). There are several reports of both retrograde and anterograde intracellular...replication like RABV or HSV. There are, however, a few reports of trans-synaptic spreading of some AAVs, specifically...

  5. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating...enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The ...reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 Listen to the podcast...

  6. Chemogenetics AAV Preps

    Type
    Collection
    ...PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...

  7. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... in sample treatment across methods can affect reported titer by either method so to ensure equal animal...AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction. While...marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and link for ...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these ...is a ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering... reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition...

  9. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

  10. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...by reducing the distance between the target and reporter. They have fewer non-specific binding opportunities...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

  11. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...As the Chair of the Advocacy Committee, I had to report to the Board of Directors and liaise with the Executive...accordingly. For example, the quarterly and annual reports I had to write on behalf of the Advocacy Committee...to overcome the personal frustration and submit reports in the format requested which in the long run was...

  12. Worm Expression Resources

    Type
    Collection
    ...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...

  13. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...expression plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific...including Protein A, 1xFLAG, 2xAM, and HA have been reported, as well as affinity purification with an anti-Cas9...chromatin immunoprecipitation (enChIP)." Scientific reports 3 (2013): 3171. PubMed PMID: 24201379. PubMed Central...

  14. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...pathway is ubiquitous, with successful recombination reported in all laboratory E. coli strains tested so far...use of this pathway as a cloning tool was first reported over 25 years ago (Douglas and Howard, 1991, Bubeck...exploiting bacterial in vivo assembly." Scientific reports 6 (2016): 27459. PubMed PMID: 27264908. PubMed ...

  15. MXS Chaining

    Type
    Blog Post
    ...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...

  16. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers...designed a tool that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral...complicating the issue is the long and ugly history of reporting false essentialist links between biology and ancestry...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    ...the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9...promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system, they could quantify and track...dCas9 targeting plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be...t common for simple transfection experiments. Reporters Finally, in some cases a researcher may choose...

  19. Lentiviral Prep Service

    Type
    Collection
    ...on each item's material page. Actual titers are reported with each shipment. For more details about our...

  20. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...primary antibody respectively. Avidin bound to a reporter is then added to the reaction for easy detection... experiments. During detection, a biotinylated reporter enzyme that is pre-incubated with free avidin ...protein of interest leading to amplification of the reporter signal and easier detection. This strategy is ...
Showing: 101 - 120 of 369 results