We narrowed to 274 results for: fas
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TypeBlog Post...enzyme. Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell...cyanobacterium Synechocystis sp. PCC6803, and the fast growing strain Synchococcus elongatus UTEX2973, ...
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Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...CRISPR plasmids New items from the viral service A fast blue-light inducible Cre for precise control of ... can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours. Sheets, MB... -
Hot Plasmids February 2024
TypeBlog Post... lines such as CAR-T cells. Plus, this method is fast! These editing frequencies can be achieved with ... to the original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post... individually assembles the reads into a single FASTA sequence that our QC scientists can easily analyze...each sample. About 24 hours later we unload the FASTQ data from our instrument and begin the analysis.... -
Tips for Technical Support Calls
TypeBlog Post...responses asking for more specifics. We can help you faster if we get as much information as possible about...one. Though an initial response should be fairly fast, getting the full answer to a complicated question... -
Pooled Library Amplifications
TypeBlog Post...smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts of... with a single size insert, liquid media can be faster and easier to work with. In either case, it can... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...cjBlue, meffBlue, aeBlue, amilCP, tsPurple, and gfasPurple) (Figure 2). Figure 2: Chromoprotein expression...combines the most desirable features: intense color, fast maturation, and low fitness cost yet. However, many... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...volume and you should ensure the shaker speed is fast enough for sufficient gas exchange in the overnight...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM)... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...bacterial and yeast cells are cost-effective and faster-growing than mammalian cells, but mammalian cells...successful. Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers... -
DNA Purification Without a Kit
TypeBlog Post...syringe filter method is not only cheaper, but also faster and more flexible than commercial kits. DNA gel...bead-based kit, this cellulose-based method was faster, cheaper, and had a similar level of sensitivity... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...first generated a multi-fasta file of the hg19 genome using Bedtools getFasta. These regions and their... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...sometimes fun needs to be had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage...means something fun is going on and to get there fast! Spread word of your plans by email, gong, evite... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...making experimental workflows considerably easier, faster, and more efficient than previous methods. Still...Sanger sequencing-based software tools and is also faster and easier to use. ICE is completely free for everyone... -
Plasmids 101: Shuttle Vectors
TypeBlog Post... to grow, clone, and manipulate their vectors in fast-growing bacteria first. In fact, most yeast vectors...you also have to consider how to keep it there. In fast-growing populations of cells, the vector needs to... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post... we contracted with so we can always offer the fastest shipping. However, there were issues that persisted...able to better understand the barriers to easier, faster, more reproducible science and learn what Addgene... -
How to Be an Excellent Trainee
TypeBlog Post...learn faster and retain more information plus your mentor will get you up to speed easier and faster when... -
Career Insights: Technical Support Specialist
TypeBlog Post...and safe corners in a company, less exposed to the fast dynamics and pressure which are often found in sales...role Talking to customers everyday, it can be fascinating and exciting to listen to the many individual... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...ready for purchase. But what is the easiest and fastest way to use the system in mice? We'll have a post... vitro first. In vitro screening is a simple and fast method for validating your selected gRNA’s, and ... -
Tips for Using FRET in Your Experiments
TypeBlog Post... old-fashioned at all. With the development of new fluorescent pairs and the emergence of faster measurement... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...used to refer to something that has fallen out of fashion but is still useful and most importantly, effective...labs. However, RNA interference (RNAi) remains steadfast in the background, a handy “oldie” as you might...