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We narrowed to 388 results for: fus

Showing: 101 - 120 of 388 results

  1. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ... the second type of these GEVIs: a FP-fused rhodopsin. FP-fusion to rhodopsin resulted in electrochromic... Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein (FP). This class...imaging.   Figure 1: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin...

  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...exact entry for your favorite gene fused to an epitope tag or fusion protein. If your sequencing primer...An alignment omitting repeated regions can be confusing, such as when you attempt to verify the starting...primer was chosen to confirm a tag or fusion protein is in-frame, we recommend using blastx with the "Align...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually does not affect ...and 2) to visualize the localization of the FP (fused to the protein of interest), thus tracking the localization...used to determine the proximity of two proteins fused to the halves of the split FP. This technique is...This may affect the biological function of the FP-fusion protein. Therefore, it is recommended to use monomeric...

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...has focused on transcriptional activation using a fusion to a transcriptional activator such as VP64 (Gilbert...transcriptional activators (Qi et al., 2013). However, the fusion of dCas9 with a protein tag allows for the isolation... DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA, were successfully... (Liu et al., 2017). This system utilizes dCas9 fused to a biotinylation tag expressed on a plasmid, allowing...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    ...modular approach combines an interchangeable set of fusogenic and targeting components to reprogram delivery...Modular features of DIRECTED particles where the fusion and targeting components are interchangeable. Image...targetable cell types. DIRECTED is compatible with fusogens from different viral families and can be used ...transcriptional history of a cell. Lin et al. generated a fusion protein called iPAK4 that assembles into a slow-growing...

  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study) are captured on...programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting...silencing, which can be toxic, CHARM uses a cofactor fused to a histone tail to recruit and stimulate the endogenous...multiplexed targets.   Figure 4: CHARM uses a fusion of the DNMT3L cofactor (D3L) with a histone tail...

  7. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...killswitch can be genetically fused to a protein of interest or targeted to a GFP fusion protein using an anti-GFP...of interest fused to GFP forms fluorescent biomolecular condensates. A GFP-nanobody fused to the killswitch...

  8. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...expression, measured using fluorescent protein fusions In addition to visible light, flow cytometers can...Which Fluorescent Protein Should I Use? However, fusion proteins tag your protein of interest with a fluorescent...- or C-terminus of the protein. One downside of fusion proteins is that the tag can alter the structure...get around the challenges of fluorescent protein fusions by binding to the protein in its native state.  ...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa of protein mass...to this bulky insertion are inevitable. Most FP fusions for FRET studies are at the N- or C-terminus of... protein of interest, resulting in a C-terminal fusion of a tetracysteine tag. In addition, since these...FRET Browse Empty Plasmids to Fluorescent Protein Fusions Check out Our Fluorescent Protein Guide ...

  10. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ... any tags or fusions that are on your insert. Typically you would list any tag or fusion protein in the... pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list ...

  11. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to an Mxi1 repressor...covalently attached to the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases HDR efficiency...

  12. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ..., Georgieva et al. examined the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags...untagged GFP overexpression, while the rest of the fusions had some negative effect on these parameters. IT5... cell morphology was observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental...

  13. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains much of the information...directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene through a 2A peptide...the 2A peptide on its C-terminus, SpCas9 is also fused to a 3X FLAG tag at its N-terminus. This tag can...

  14. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...they identified included gene amplifications and fusions, missense mutations, and indels (Ng et al., 2018...carrying a single gene with a defined mutation or gene fusion. Plasmids carrying the corresponding wild-type ...Mutations in Cancer.” Of all the mutations and fusions represented in this collection, approximately 80%...

  15. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays in the cytoplasm...vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional regulators. In this...

  16. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ... for Goldengate cloning   Design primers for In-Fusion Cloning Design primers for Gibson Assembly and ...restriction enzyme cloning, Gibson assembly, or In-Fusion cloning.  NEB Ligation Calculator: This tool works... from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators Lab math calculators...

  17. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...an exon of a gene to generate a transcriptional fusion. This may disrupt the endogenous function of the...the gene product resulting in a transcriptional fusion, much like promoter traps. While this system does...disrupt the gene it monitors due to transcriptional fusion.   Examples of promoter, enhancer, and gene...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...by using the DNA cutting activity of a Protein A fused micrococcal nuclease (MNase) to specifically isolate... against the protein of interest. The Protein A fused MNase is then added and Protein A binds Immunoglobulin...protein interactions Learn about other types of fusion proteins Study chromatin interactions with CAPTURE...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...biosensors contain a single fluorescent protein fused to a domain that senses the concentration of a given...an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin kinase... an intracellular or extracellular molecule? By fusing a biosensor to an organelle-specific targeting ...

  20. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...available – you can always add an epitope tag or a fusion protein. Mammalian vectors One of our most popular... cells. The addition of an in-frame fluorescent fusion protein will allow you to visualize YGOI in vivo... offer a collection of vectors carrying several fusion proteins. Pick your promoter, selection cassette...
Showing: 101 - 120 of 388 results