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... post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...

...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

... C. elegans, but activation of Raf-MEK-ERK signaling in the hypodermis and gut cells of C. elegans causes... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases...utility of  photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection...Zhou et al also used a photoswitchable CDK5 in C. elegans. See figures 4B and C in Zhou et al for details..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...

...synaptic proteins Fluorescent protein system for C. elegans Fluorescent timers for studying cell cycle length...fluorescent proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due...locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since it requires ...use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet...they fuse and fluoresce. The team engineered C. elegans cell lines to express wrmScarlet1-10 unattached...

...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

...been an invaluable resource for studying the organization and function of cellular systems. FPs have been...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular... by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging with SapTrap The Jorgensen...SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one tube...tagging systems For those who do not work with C. elegans, several groups have designed modular toolkits ...CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4) (2016):1277-88. PubMed PMID:...

...annotating, and storing life science protocols. Since we began sharing this protocol on the Addgene website in ...About Developing Lab Management Software    Organizing and sharing your updated protocols So that’s ... mobile) tools are available for scientists to organize and share their scientific protocols. Here are... various academic labs, and now Addgene. I just began uploading our cloning protocols to the site and ...streamline your lab management and you want the works: organizing reagent storage and inventory, managing orders... can offer a one-stop solution to your lab's organization and management needs. With Quartzy you can share...

...protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in...selective interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded...specifically and irreversibly with a small substrate ligand motif attached to a fluorophore (Figure 1b). ...catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry...labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free in solution...the availability of fluorescent and fluorogenic ligands. New fluorophores As a corollary to these innovative...

...Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), some 1010 oligonucleotides...of the next two decades, these nucleotide-based ligand binders would prove to be highly adaptable tools...structure (see Fig. 1a). When the aptamer binds its ligand, the stem loop is disrupted, removing the quencher... dimerization for activation, only in this case ligand binding would bring the relevant ends together ...aptamers fluoresce until they bind to the appropriate ligand, but relying on a lack of fluorescence for readout...response. Aptamers can be selected for these small ligands. References 1. Ma, Hanhui, et al. “Multicolor...

...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...serve you! Let us know about other organisms we should include by emailing us at [email protected]. If...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...like to get the word out about a particular microorganism, microbial technique, or microbial technology...

...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...

...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be familiar...chromosomes during mitosis, support transport of organelles and vesicles, and more. Although several strategies...that means this light-powered sword truly is an elegant tool. The authors demonstrated its use to probe...family proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and...forming the cilia that help establish left-right organization of the embryo (Aljiboury et al., 2023). It’s...and cilia formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https...

... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...your gene of interest into a target cell line or organism (Presented in figure 1). More information on Agrobacterium-mediated...

...through your organization in this field. Please also enter the billing address for your organization. We will...By Natalie Mortenson and Laura O'Regan Ordering jeans online from your favorite store is easy. Ordering...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...

...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

...as a free event! Organizing a science conference for the public How does one organize a public scientific...association with the Harvard graduate student organization Science in the News (SITN), we recently sponsored...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...opportunities to improve our communication and organizational skills provide great training for a number ...

...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm....

...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...