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Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Learn about hot new tools in our collection!

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!

CRISPR plasmids for endogenous tagging of your gene of interest.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

CRISPR/Cas plasmids for labeling and visualization of genomic loci.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

A round up of guest blog posts of 2024. Thank you to our guest contributors!

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

Scientists at Beam Therapeutics have found a way to improve editing in primary human cells using evolved adenine base editors called ABE8.

CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).

CRISPR expression plasmids that have been designed for use in parasites.

CRISPR plasmids for activating or upregulating gene expression in various model organisms.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Find prime editor plasmids for (search and replace) genome editing.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

Catalytically inactive CRISPR plasmids for purification of target loci.