We narrowed to 213 results for: grna

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

A round up of guest blog posts of 2024. Thank you to our guest contributors!

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

Scientists at Beam Therapeutics have found a way to improve editing in primary human cells using evolved adenine base editors called ABE8.

CRISPR plasmids for endogenous tagging of your gene of interest.

CRISPR/Cas plasmids for labeling and visualization of genomic loci.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).

CRISPR expression plasmids that have been designed for use in parasites.

CRISPR plasmids for activating or upregulating gene expression in various model organisms.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.