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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 287 results for: lum

Showing: 101 - 120 of 287 results

  3. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...management software aren’t just limited to large volume facilities; it can be useful in academic labs where...collision. It may even be worth it to have extra columns in your database to the effect of `dev_name` and...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...reprobing works best with western blots that use chemiluminescent or fluorescent detection, like horseradish...remains, whether that involves adding fresh chemiluminescent substrate or imaging your fluorescent probe...

  6. Of Myc and Men

    Type
    Blog Post
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

  7. Fluorescent Proteins: FRET

    Type
    Collection
    ...paired with a fluorescent protein acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's...

  11. Plasmids 101: Protein tags

    Type
    Blog Post
    ...metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused...with the cleavage reaction taking place between column runs. The TEV protease itself, with various mutations...

  12. COVID-19 Resources

    Type
    Collection
    ...nonprofits, and this is denoted in the industry column below. For more information on ordering...

  13. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ... through this same initial QC process using an Illumina MiSeq, but that’s not the only technology Addgene...  Bioinformatics at Addgene After a run on our Illumina MiSeq, the data is imported into our assembly ...

  14. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

  15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...

  16. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...
Showing: 101 - 120 of 287 results