...collection of PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic fusion...laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent modules, such...Gross RE. (2015). Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable...systems in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic or optogenetic...Augustine GJ, Yin HH, Hochgeschwender U (2016). Luminopsins integrate opto- and chemogenetics by using physical... a single molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 ...Shaner NC, Hochgeschwender U (2024). Engineering luminopsins with improved coupling efficiencies. Neurophotonics...

...sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2 photoswitch. Illumination causes...expression of the opsin and the area that is being illuminated. There are several different ways to control ...2006. Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci...

...acquire NGS but one of the most commonly used is the Illumina NGS platform. This is the platform used by Addgene...sequencing partner, seqWell . The actual process of Illumina NGS is not that different from Sanger sequencing...modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented into...regular base and thus do not halt the reaction. Illumina NGS, therefore does not require any “normal” bases...

...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced... protein extract by centrifugation or affinity columns. Mix samples with a primary antibody, which is ...

...with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes...