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We narrowed to 6 results for: lum

Showing: 1 - 6 of 6 results

  1. Chemogenetics Guide

    Type
    Guide
    ...collection of PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic fusion...laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent modules, such...Gross RE. (2015). Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable...systems in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic or optogenetic...Augustine GJ, Yin HH, Hochgeschwender U (2016). Luminopsins integrate opto- and chemogenetics by using physical... a single molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 ...Shaner NC, Hochgeschwender U (2024). Engineering luminopsins with improved coupling efficiencies. Neurophotonics...

  2. Molecular Biology Reference

    Type
    Guide
    ...acquire NGS, but one of the most common is the Illumina NGS platform for short-read sequencing. This is...plasmids deposited at Addgene. The actual process of Illumina NGS is not that different from Sanger sequencing...modified, fluorescently-tagged nucleotides. During Illumina NGS, a long piece of DNA is fragmented into small...regular base and thus do not halt the reaction. Illumina NGS, therefore does not require any “normal” (... of DNA can be sequenced. Figure 4: Summary of Illumina NGS. Created with BioRender.com. At Addgene, all...research. Long-read Sequencing Sanger sequencing and Illumina NGS are limited to shorter reads of DNA, usually...

  3. Optogenetics Guide

    Type
    Guide
    ...sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2 photoswitch. Illumination causes...expression of the opsin and the area that is being illuminated. There are several different ways to control ...2006. Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci...

  4. Antibody Guide

    Type
    Guide
    ...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced... protein extract by centrifugation or affinity columns. Mix samples with a primary antibody, which is ...

  5. Modular Cloning Guide

    Type
    Guide
    ...variety of applications. EcoFlex MoClo Extension, Volume I Bacterial Expression Mikhail Shapiro 96 cross-compatible...expression tuning in E. coli . CIDAR MoClo Extension, Volume I Bacterial Expression Richard Murray 110 plasmids...

  6. CRISPR Guide

    Type
    Guide
    ...with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes...
Showing: 1 - 6 of 6 results