...expression plasmids (AsCpf1 and LbCpf1) for use in mammalian cells SpCas9 variants with no detectable off-target...

...cancer development. These pathways are important to normal cell signaling and physiology, and, when dysregulated...

...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...difficult-to-target antigens that are intractable via animal immunization. Addgene's antibody catalog is a community...

...difficult-to-target antigens that are intractable via animal immunization. IPI is a nonprofit research organization...

...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a 5, rg* Heintz , Nectow , Schmidt Neurophysiology...

...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...Autophagosomes 101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T...

...Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury MP,...

...include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into...

.... 2014. Reprogramming adipose tissue-derived mesenchymal stem cells into pluripotent stem cells by a mutant...

...neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies ...

...pAquaN1 Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a ...compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial...pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet...Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses...N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus...C-terminus of monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular...Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus...

... yeast two-hybrid assay are not optimal for studying native mammalian protein interactions, whereas others...is needed to assess its function in mammalian cells or other animal models, but this technique has laid...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334...sheared genomic libraries are cloned downstream of a minimal promoter sequence. Should one of these random stretches...it will activate its own transcription from the minimal promoter and its strength can be determined from...RAS Pathway Clone Collection 2.0 Ras proteins are small GTPases involved in cell signaling pathways that...been difficult to target therapeutically due in no small part to the central role Ras proteins play in cell...

...expression in neuronal cells. U6 small RNA expression shRNA From the human U6 small nuclear promoter Constitutive...expression) you need to use an RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP...Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters ...leakiness" of transcription may occur. This is normally not a big issue for cells, but it may confound...listing some of the most common bacterial and mammalian promoters. These lists is by no means exhaustive...considerations CMV General expression mRNA Strong mammalian expression promoter from the human cytomegalovirus... types. EF1a General expression mRNA Strong mammalian expression from human elongation factor 1 alpha...

... by the heat. Isolating male and female flies Differentiating male and female flies is essential for setting...of their abdomens, males have black coloration whereas females do not. Also, males have sex combs on their...is important to isolate females from males while they are still virgins. Females begin mating ~6 hours ...trying to get males to recombine two mutations onto the same chromosome. You should use females for that. ...their forelegs which are black, bristly hairs. Females do not have sex combs. To make genetic crosses ... bodies. This is the ideal stage to select for females because you can be certain that they are too young...fourth chromosomes, you are a very unlucky person. Animals are diploid except for their germ cells (sets of...

...screening 768 F1 animals). Recovering rare editing events in a sea of unedited animals struck me as a “...from 11-100% of F1 animals carrying precise knock-ins, and homozygous knock-in animals can be obtained in... in eight days. The only animals on a plate, other than the parental animal, are rescued progeny, which...for any selective marker to remain in the edited animal. Development of CRISPR/Cas9 editing strategies...must be removed, which can be done by isolating animals with particular visible phenotypes. These approaches...conditional-lethal mutation to identify edits with minimal screening effort. Selecting for repair of a lethal...unedited “haystack”, facilitating recovery of edited animals. Figure 1: In the background are 504 restriction...