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We narrowed to 560 results for: mme

Showing: 101 - 120 of 560 results

  1. How to Write a Scientific Review Article

    Type
    Blog Post
    ...references organized from the beginning. I also recommend using the citation style of (Last name, Year) ...about having to hunt down sources later on.  A comment about writing: initially I felt that I needed a...get done between office hours and experiments. A comment about citing: I wasn’t sure if I should cite reviews... be revolutionary, but you could include a few comments on where you think the field is going or what ...

  3. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...highlight these recent advancements and offer commentary on their application to neuroscience research...To highlight this advantage, they selected the immediate-early gene Bdnf, which encodes brain-derived neurotrophic...early in response to neuronal activity (hence, “immediate-early”) and consequently regulates neuronal physiology...and Bdnf IV overexpression variants were other immediate-early genes associated with neuronal activity,...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...neocortex of a living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological Studies) ...oligomeric state of a fusion partner. Therefore, we recommend using miRFPs and their enhanced versions named...detection of protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova...Biol 20, 1078-1086 (2013). Shcherbakova, D.M., Cox Cammer, N., Huisman, T.M., Verkhusha, V.V. & Hodgson, ...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...data. Finally, let me take this opportunity to recommend all the science and career talks at iBiology which... careful. 3. Tell a story We are biologically programmed to listen and remember better when we hear material...directions. Don’t worry if your work hasn’t been commercialized, just talking about the possibility is interesting...sharing model works. See if you can elicit helpful comments from your audience as in “Does anyone else have...

  6. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...bacteria. It’s hard to count the number of different commercial strains of E. coli currently available – a quick...deadly to humans. The majority of all common, commercial lab strains of E. coli used today are descended... E. coli strains used in the lab Most of the commercial strains you find today are marketed for a specific...make these features possible are present in most commercial strains, especially mutations that make major...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...IF labeling of rat brain samples from the James Trimmer Lab, using recombinant antibodies with different...fluorescently conjugated secondary antibodies are commercially available. Pick whichever combination of host...questions or any favorite tips and tricks, leave a comment below. Happy imaging! References and Resources...Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...excited to do what they do. I would also highly recommend talking to other undergraduate researchers, as...point ties into the previous one too. I would recommend applying for multiple positions—don’t put all ...Experiences for Undergraduates (REUs) and other summer research opportunities are also fantastic for broadening...friends and found incredible mentors through my own summer experience. Also look into presenting your research...

  9. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...settings of the equipment. Systematic evaluation of commercially available dyes with different excitation wavelengths... the photons emitted. For example, DAPI is much dimmer than Alexa Fluor 488, and thus it is more difficult...Sridharan et al., 2014). Manufacturers will typically recommend specific fluorescent donors and acceptors in their...Marketing Manager at Promega. His work focuses on commercializing their cell and protein analysis products. You...

  10. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ... Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope...because reagents could be purchased directly from commercial suppliers without investing time and labour on... gene-specific crRNAs and matched ssODNs from commercial suppliers of CRISPR reagents (turnaround time...method. We anticipate that our method will open up immense possibilities to delineate the function of large...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... for deposits of less than 10 plasmids but we recommend using the spreadsheet option for deposits of 10... been approved. The spreadsheet option is recommended for depositing 10 or more plasmids. (If you believe... lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable strain. For plasmids containing...containing the ccdB gene, such as Gateway vectors, we recommend the ccdB Survival strain. Figure 10: Enter the...

  13. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...Readouts Toj, a summer intern who shared their experience working at Addgene. An Addgene Summer Internship...Flow Panel, and Beyond Surface Labeling Ashlyn Lemmen, who shared her flow cytometry expertise in two...

  14. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...If you have any comments about the Hot Plasmids Podcast Segment, or have recommendations for new Hot Plasmids...Plasmids topics, let us know in the comments section below. Hot plasmids covered in this segment Hot Plasmid...

  15. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ...bacterial cultures (i.e. 2-3 ml) with or without commercially available kits and DNA for transformation doesn... required sizes of the bacterial cultures and commercial kits you will adopt for the extraction. Restriction...and outcomes of experiments. For these reasons commercially available kits provide extra washing steps to...

  16. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...intellectual property rights, pending publications, and commercial use often come up. An MTA can resolve those questions...that recipient may not use the material for a commercial purpose Assurance that recipient may not use...inventions Right to publish without editorial comment or review by the Provider Limited liability ...

  17. Reflections and Looking Ahead

    Type
    Blog Post
    ...D. W., Kumaran, R., Rego, M., Schofield, C., … Laflamme, C. (2024). A consensus platform for antibody ...10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers,...Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F.,...

  18. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...comprehensive job description for this co-op, I was immediately interested in applying. This had stood out to...listed with a much less specific overview. It was immediately clear that Addgene wanted to ensure applicants...Letter 7 Tips to Secure a STEAM Internship This Summer  ...

  19. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...periodically beat their tails left and right in a symmetrical motion to move forward. This behavior is not ...Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow forward... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...

  20. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...to people outside of academia. Here are my recommendations on some ways to get started sharing your science...others in the science blogosphere is to read and comment on their blogs. 5)   Local science museums are ... research by guest blogging here, just leave a comment if you're interested and our team will get back...
Showing: 101 - 120 of 560 results