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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 147 results for: pam

Showing: 101 - 120 of 147 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Within the editing window -50 to +50 bp from the PAM site, CRISPR-X produces point mutations at a rate...

  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...study alpha2-AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is... increased selectivity for norepinephrine over dopamine. All three sensors, however, did display some ...some reaction to high concentrations of dopamine. This cross reactivity will need to be accounted for in ...

  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...adventure. Many thanks to our guest writer, Pamela J. Hines, PhD! Pamela J. Hines is a Senior Editor at Science...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...French horn, and rhubarb. Follow her on Twitter @Pam_Hines and LinkedIn. Additional Resources on the Addgene...

  4. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies... different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049...

  5. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...experiment, ChRger2 was non-invasively delivered to the dopamine producing neurons in the ventral tegmental area...were then allowed to “self-stimulate” their VTA dopaminergic neurons by triggering a burst of light that ...poked their nose into a hole in their cage. Since dopamine is an important neurotransmitter that promotes...behavior, if the channelrhodopsins activate the dopaminergic neurons in the VTA, this should propel the mice...

  6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...anesthesia. In Cre transgenic mice, they targeted the dopamine (DA) neurons of the ventral tegmental area (VTA...for encoding aversive stimuli in the mesolimbic dopamine system." Neuron 101.1 (2019): 133-151. PubMed ...Juarez, Barbara, and Ming-Hu Han. "Diversity of dopaminergic neural circuits in response to drug exposure..., Norman E., et al. "Optogenetic activation of dopamine neurons in the ventral tegmental area induces ...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...fragment with FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...Cas9 for genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES fragment is actively shuttled...to the nuclear export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether...Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department of Systems Biology at ...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... The strategy relies on several observations: Rapamycin is a membrane-permeable pharmacological agent ...induction heterodimerization of proteins that contain rapamycin-binding domains, such as FKBP and FRB. The cell...membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) and ...

  11. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...without a personal message and they may report you as spam or “I Don’t Know This Person.” If that happens a...the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...monosynaptic tracing to identify inputs to two major dopaminergic cell populations in two specific brain regions...Whole-brain mapping of direct inputs to midbrain dopamine neurons. Neuron. 2012 Jun 7;74(5):858-73. Pubmed...

  15. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...including next-generation biosensors for GABA, dopamine, serotonin, and much more. Check them out! Table...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...a wide array of neurotransmitters ranging from dopamine to glutamate, all the way to acetylcholine. These...
Showing: 101 - 120 of 147 results