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We narrowed to 134 results for: plasmid transfection

Showing: 101 - 120 of 134 results

  1. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and...These are transfected into target cells which produce lentiviral particles.   Why pseudotype? Lentiviruses...scientists encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism...

  2. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols...Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction ...Name Description Link to Video General Transfection Introduce plasmid DNA to mammalian cells Lentivirus Production...Antibodies Name Description Link to Video Transfection Introduce plasmid DNA to mammalian cells to produce antibodies... that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification...spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis...

  3. Antibody Plasmid Collection

    Type
    Collection
    ...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...Recombinant mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid... Plasmid Collections...

  4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...viral entry into the host cell.  Transfecting these three plasmids into cells enables the production ...integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details on how to...lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long...this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically...production of lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus... vector using an integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

  6. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

  7. Brain Initiative Collection

    Type
    Collection
    ...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...research. Browse the BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...dissemination to the research community. Browse plasmids , viral preps , and antibodies created with support...distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as high-quality...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage reporter...IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR...IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common internal... be used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...protein (Wang et al., 2013). These proteins are also plasmid-derived. However, a disadvantage to ScFv is their...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies... as well as tools for developing and producing plasmid-based antibodies - and we’re always growing our...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

  9. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ... as early as 48-72 hours post-transformation/transfection in a transient assay. For stable transformation...

  10. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... protein expression observed using transient transfection approaches, generating cell lines using lentiviral... stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it eliminates...variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian antibiotic...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers...lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming...Tools Read other CRISPR blog posts Find CRISPR Plasmids at Addgene  ...

  12. Viral Vectors

    Type
    Collection
    ...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...are usually divided among many plasmids, such that all of these plasmids must be present for a viral particle...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets the plasmid contains all of the...rectangles) are cloned into various plasmids using oligonucleotides. These plasmids contain Type IIS restriction...different purposes.   Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with...assembled to produce a plasmid encoding the gRNAs and Cas9. As with the Gersbach lab plasmids, multiple Cas9 ...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids allow you to assemble 2-4 gRNAs through...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids can be used to express up to 8 gRNAs after...Yang Lab Single Transcript Multiplexing Plasmids: These plasmids are similar to the Csy4 polycistronic ...

  14. Viral Production

    Type
    Collection
    ...Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding Rep and serotype-specific... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources... serotype-specific Cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...microbial contamination. Purity When possible, all plasmids used for viral production are propagated in the...experiment using our virus protocols . Browse our plasmid collection of popular lentiviral vectors ....

  15. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    ... With the ability to produce neuron specific transfection using Cre-lox, this new method could allow for...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  17. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent...Fluorescent Biosensors Chemogenetics Plasmid Collection Optogenetics Plasmid Collection Monosynaptic Neuronal... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout... Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources ...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...

  19. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic...Additional resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our...

  20. Plan Your Experiment

    Type
    Guide
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...into mammalian cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...cells. These methods are more transient than plasmid transfection or viral vectors, reducing the risk of accumulating...difficult to transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...
Showing: 101 - 120 of 134 results