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We narrowed to 416 results for: plasmids 101

Showing: 101 - 120 of 416 results

  2. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...generate stable cell lines. Packaging and Envelope plasmids provide the necessary components to make lentiviral...for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro ...efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR ...User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems Check out our CRISPR Guide... crispr 101...

  3. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101: Viruses...their required components are very similar. While plasmids containing virus-specific elements such as the...integrate, and the addition of a helper virus or plasmid allows it to replicate episomally, essentially ...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S., et al. Adenovirus-Mediated...Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig T, Galla M, Baum ...

  5. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...Ligation Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost effective...Resources on the Addgene Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques Additional... Additional Resources on Addgene.org Find Plasmid Cloning Protocols Find Virus Protocols Find Basic Molecular...of large gene clusters. Nat Commun. 2015; Sep 1;6:8101. PubMed PMID: 26323354. PubMed Central PMCID: PMC4569707...

  6. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...feature some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation...past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These...s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent... about mNeonGreen, mTFP1, and mWasabi and find plasmids containing these fluorescent proteins at Addgene...graphics. PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection...

  7. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

  8. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each HIS Own...

  9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ... Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...protein fusions Browse the fluorescent proteins plasmid kits ...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...Proceedings of the National Academy of Sciences 101.29 (2004): 10554-10559. PubMed PMID: 15247428. PubMed...

  10. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    ...resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...to share the latest edition of our Viral Vectors 101 eBook!  This practical guide to using viral vectors...

  11. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect...Addgene's Antibody Plasmid Collection Resources on the Addgene blog Antibodies 101: Reading a Flow Plot...Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

  12. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...on the Addgene blog Antibodies 101: Epitope availability Plasmids 101: Protein Tags Antibodies: Of Myc...

  13. Pouring LB Agar Plates

    Type
    Protocol
    ...also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry ...resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid from bacteria...resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. The following...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...

  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...

  15. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our most...our CRISPR 101 eBook and the upcoming release of the second edition of our Viral Vectors 101 eBook. We’...factor library, in lentiviral vectors. At 3500 plasmids, this represents the largest single deposit in...to announce that we are now able to ship rabies plasmids in our repository to international requestors....

  16. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ... Antibodies 101: Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

  17. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... (more on when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken...Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional resources on the...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent... ligands by exponential enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity...

  19. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Read the paper in Science Find the new SHERLOCK plasmids More precision with dual-nuclease Cas9-Cas9 ...Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants...post Read the paper in Science Find the Cas14 plasmids Anti-CRISPR proteins for optogenetic control...paper in Nature Methods Find the anti-CRISPR plasmids Tagging human transcription factors with GFP...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... New to viral vectors? Browse our Viral Vectors 101 blog posts Read blog posts about neuroscience Resources...viral preps for your research Find chemogenetics plasmids for your research ...Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Saloman, J. L., Scheff, N....
Showing: 101 - 120 of 416 results