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...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

...This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. ...e-magazine this year which would feature articles, posts, and sci-art that would ultimately give a visual...A contribution can either be an article, a short post, a video, or a piece of sci-art.  What are your ...Addgene blog Find all of our science communication posts Sign up to receive our science career guide Learn...science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive... Sandangi! Dr. Chinmaya Sadangi is currently a postdoc at the University of Toronto and founder of The...

...have so many blog posts about careers that we’ve compiled our top career blog posts into one comprehensive...writing a guest post. Additional resources on the Addgene blog Read all careers blog posts Read our transferable...

...In this post of the Careers in Science Communication blog series, you’ll hear from Susan Keown, a staff...skill set wasn’t matching up with the local job postings. “There were jobs that I didn’t seem to have the...Twitter feeds and dealing with social messages and postings,” says Susan. There are also team meetings and...often easier to accept unpaid work as a student. For post-grad school individuals, Susan recommends starting...the blog so you don’t miss out on future career posts! Additional Resources on the Addgene Blog Volunteer...Communication Check out Other Science Communication Posts Resources on Addgene.org Explore careers at Addgene...sitting in my office one day, which I shared with a postdoc, and I’m like, “Oh my god, what can I do?! This...

...keeps changing, we’ve been updating many of these posts over the last several months. Now the #12DaysOfCRISPR...Twitter where we shared one of these updated blog posts each day. If you missed it, here’s the recap! The...series. Be sure to subscribe to the CRISPR blog posts so you don’t miss a thing! ...

...This post was contributed by guest blogger, Gary McDowell, executive director of Future of Research. ...increasing from $23,660 to $47,476 per year. This post discusses how a nationwide injunction against the..., while 69% of all postdocs were expected to receive salary raises, 6% of postdocs were to have either...tracked, 3% of postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...on the morale of postdocs, and indeed we have been contacted by a number of postdocs who are now planning...salaries, the only postdocs we knew for certain to still be getting their raise were: postdocs at institutions...carry over into other aspects of postdocs’ lives. We know that many postdocs already made plans and budgets...

...Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some are...researchers from the Boehm and Beroukhim labs to write a post on their paper uncovering ancestral bias in CRISPR...targeting to animal models. It was one of our favorite posts from 2022! Wow! Our depositors do some really incredible...

...This post is part of our ongoing Plasmids 101 series. Plasmids 101 will provide you with an overview ...more, you can find the rest of the Plasmids 101 posts here. Now that we have covered antibiotic selection...strategies, check out our screening overview blog post.) *Note: Two Popular Vectors for Blue White Screening...phenotype. Resources on the Addgene Blog Read Our Post on Common Lab E. coli Strains Learn to Use Yeast...Two Hybrid Systems Browse All of Our Plasmids 101 Posts Resources on the Addgene Website Choose the Best...

...Read our blog posts on open science Find blog posts about scientific sharing Find blog posts about networking...protocols, clinical information, phage research job postings, and phage news into the Phage Directory website...

...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

...first post in a three-part series. We’ll first discuss how to get started on fly work in this post. The...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....The second post will detail a major tool used by Drosophila geneticists (the Gal4/UAS system), and the ...the third post will describe how you can make your own mutant flies. Find Drosophila Resources at Addgene...gene knock down and gene expression. In the next post, you’ll learn the fundamentals about how many of...

...screens, see our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries...

...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

...scientists up to on Twitter? Prior to writing this post, my interest in Twitter was fleeting. I’ve had an...8 tweetable facts. Note: The images used in this post were created using data from or modified from Ke...Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains more often ... use social media as a scientist? Check out this post. Resources on the Addgene Blog 6 Tools & Tips:...

...selection in this blog post, but don’t worry, we’ll cover screens in a future post. Positive selection...naturally occurring genes  At this point in the blog post, you may have realized that positive and negative...on the Addgene blog Read our Plasmids 101 blog posts Learn more about Lambda Red recombineering Learn...

...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...million plasmids shared milestone in 2018, we wrote a post on the time and cost required to make a plasmid.... not!), we think it’s about time we updated this post. How much time does it take to make a plasmid?...precise calculations for any of the numbers in this post is a mathematical story well beyond my ability to...help you choose the right cloning method Find blog posts about scientific sharing here Download the Plasmids...save yourself hours by chatting quickly with the postdoc who’s really good at primer design – but first,...

...antibiotic resistance marker). In a previous blog post, we covered how to use positive and negative selection...are blue. Check out our blue-white screening blog post for more information.   Figure 1: Result of... process, or visit our restriction analysis blog post. Colony PCR Colony PCR can detect the presence...details and learn more about colony PCR in this blog post.   Figure 2: The steps of colony PCR.   ...either terminus. Want to know more? Visit this blog post for tips for analyzing and troubleshooting Sanger...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...

...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance...of plants and animals. Unlike bacteria, yeast can post-translationally modify proteins yet they still share...Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed how the regulation of bacterial...

...biology blog posts Find tips for Arabidopsis transformation Read our Plasmids 101 blog posts Resources ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with...transient technique that uses a virus and the plant's post-transcriptional machinery to silence genes of interest...MoClo system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo...and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene silencing method." International...

...CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts Resources...seem endless - and overwhelming. In the first blog post in this series, I’ll highlight terminology you should...mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing and maintaining...