We narrowed to 753 results for: rest

... easily gate on the unlabeled cells of interest while the rest can be excluded, or “dumped”. From left...fluorophore-conjugated antibodies directly bind to epitopes of interest that are found in the extracellular space. The...like bulk lympho-/splenocytes, you might only be interested in one of the various cell populations; for example...selection: labeling markers that your cells of interest do not have, and selecting for the cells that ...quick method of negatively selecting your cells of interest is magnetic bead-activated cell sorting, or MACS...An effective strategy to sort out your cells of interest via FACS using negative selection is called dump...Intracellular labeling Oftentimes, a marker of interest is not located on the cell membrane but inside...

...the system is to infect the cell population of interest with a deletion-mutant tracing virus that lacks..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

...plasmids for a wide array of research purposes. Interested in Addgene's viral services? See Addgene's Packaged...these viral vectors are used to transduce cells of interest. Specific modifications to these plasmids, such...

...recommend that you plate 50 μL on one plate and the rest on a second plate. This gives the best chance of...also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation... how to transform E. coli with your plasmid of interest. Protocols...

...microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses, protozoa, fungi,... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...

... viral biology without disturbing the virus of interest and monitoring endogenous protein dynamics in ...interaction (PPI) studies, where each protein of interest is fused to either LgBiT or SmBiT and expressed...

...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...vertebrate brain - Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish...

...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...therapeutic strategies to treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in...

... the experiment, preventing contamination of the rest. Avoid long exposure of the cell culture to air ...

...affinity is much closer to the Ca2+ concentration in resting neurons. Their soma-targeted variant SomaFRCaMPi... MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023). The power and potential of mitochondria...killswitch can be genetically fused to a protein of interest or targeted to a GFP fusion protein using an anti-GFP...killswitch-nanobody system. A cell expressing the protein of interest fused to GFP forms fluorescent biomolecular condensates...

...out my window and I saw the Dorr Mountain with the rest of the skyline of Acadia National Park. I was confused...molecular biologist with Biogen and is particularly interested in applying CRISPR for mouse genome editing. ...Zoology, Chinese Academy of Sciences, and he is interested in using CRISPR to develop therapeutic applications...

...release the plunger, allowing it to go back to its resting position. Wait for the liquid to stop flowing into...

...so we’ll focus on DNA transposons for much of the rest of this article. Class I Transposable elements (...transposase gene is provided in trans so that a gene of interest can be inserted between the transposon’s LTRs,...mini-transposon that contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences...

...exhausting. Now is the time to develop strategies to rest and re-energize yourself, and to address all of ...better idea of how to leverage it to ask new and interesting questions in your own lab. 2. Seek multiple mentors...investigators. Discussions can reveal mutual scientific interests, complementary perspectives, a shared communication...Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory proteins...

...plasmid. This is seen only in the uncut DNA; the rest of the digest appears normal and produces the expected...When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our...themselves cannot be used to detect multimers, since restriction sites in multimers usually repeat in tandem along...DNA without changing the size. As a result, a restriction digest of multimers will produce the same fragment...sample, you could try linearizing the plasmid via restriction digestion and then run the linearized plasmid...eBookAddgene's Molecular Biology ReferenceAddgene's Restriction Digest Protocol More resources on the Addgene...

...regions are made from the Ig heavy chains, and the rest of the Fab regions are completed with the Ig light... of interest from among the mixture and to measure the relative amount of your protein of interest on ...scientists to target specific proteins they’re interested in. Once a protein is targeted with an antibody...primary antibody, which recognizes your protein of interest, and a secondary antibody, which recognizes the... A primary antibody recognizing the protein of interest is added, and will bind to that protein on the... antibodies are used to capture the protein of interest and detect its presence. Then, the amount of light...sample, you’ll want to extract your protein of interest from the mixture. Antibodies can do this, too!...

...presenting cells (APCs) that would normally alert the rest of the immune system to the presence of cancer are... complicated and dynamic phenomenon that is interestingly responsible for tumor reduction as well as progression...TGFβ. T-regs are suppressive immune cells that restrict the production and activity of other effector ... engineering and cancer immunotherapy, and is interested in exploring new avenues in healthcare.   References...

... monoclonal cell lines by limiting dilution. The rest of the cells were frozen down. Figure 1: ...

...variants of LRRK2 , SNCA , and other proteins of interest. Browse or search the table below. ID Plasmid ...

...research applications. Browse Antibodies Production Interested in our production process? At Addgene, we provide...