... molecules associated with a genomic region of interest in vivo is essential to understanding locus function... molecules associated with a genomic region of interest in vivo. For CRISPR-based purification, design...

...localization and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find...Subcellular Localization Determine where your protein of interest resides by using a well-characterized fluorescent...

...repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander ...Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional activator...

...the system is to infect the cell population of interest with a deletion-mutant tracing virus that lacks..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

...microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses, protozoa, fungi,... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...

...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...vertebrate brain - Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish...

...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...therapeutic strategies to treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in...

...so we’ll focus on DNA transposons for much of the rest of this article. Class I Transposable elements (...transposase gene is provided in trans so that a gene of interest can be inserted between the transposon’s LTRs,...mini-transposon that contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences...

...regions are made from the Ig heavy chains, and the rest of the Fab regions are completed with the Ig light... of interest from among the mixture and to measure the relative amount of your protein of interest on ...scientists to target specific proteins they’re interested in. Once a protein is targeted with an antibody...primary antibody, which recognizes your protein of interest, and a secondary antibody, which recognizes the... A primary antibody recognizing the protein of interest is added, and will bind to that protein on the... antibodies are used to capture the protein of interest and detect its presence. Then, the amount of light...sample, you’ll want to extract your protein of interest from the mixture. Antibodies can do this, too!...

...plasmid. This is seen only in the uncut DNA; the rest of the digest appears normal and produces the expected...When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our...themselves cannot be used to detect multimers, since restriction sites in multimers usually repeat in tandem along...DNA without changing the size. As a result, a restriction digest of multimers will produce the same fragment...sample, you could try linearizing the plasmid via restriction digestion and then run the linearized plasmid...eBookAddgene's Molecular Biology ReferenceAddgene's Restriction Digest Protocol More resources on the Addgene...

...exhausting. Now is the time to develop strategies to rest and re-energize yourself, and to address all of ...better idea of how to leverage it to ask new and interesting questions in your own lab. 2. Seek multiple mentors...investigators. Discussions can reveal mutual scientific interests, complementary perspectives, a shared communication...Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory proteins...

... or by RT-PCR. Isolate your PCR product from the rest of the PCR reaction using a kit, such as the (Link...assist with restriction enzyme digestion (usually 3-6bp). Restriction Site: Your chosen restriction site for...fusing the upstream restriction site to the forward primer and the downstream restriction site to the reverse...product is now ready for restriction digestion. Digest Your DNA Set up restriction digests for your PCR product... Plasmid Cloning by PCR You may also like... Restriction Digest of Plasmid DNA DNA Ligation Bacterial ...of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that...that it can be easily cloned into a plasmid of interest. For this example, we will describe how to copy...

...you to create fusion proteins with your gene of interest....you to create fusion proteins with your gene of interest. These are organized By Color of the fluorescent...

...presenting cells (APCs) that would normally alert the rest of the immune system to the presence of cancer are... complicated and dynamic phenomenon that is interestingly responsible for tumor reduction as well as progression...TGFβ. T-regs are suppressive immune cells that restrict the production and activity of other effector ... engineering and cancer immunotherapy, and is interested in exploring new avenues in healthcare.   References...

...cells that are then screened for a phenotype of interest. Screening experiments using a pooled CRISPR library...individual library page. Find Pooled Libraries of Interest Click on different properties to create a custom...

... variants of LRRK2, SNCA and other proteins of interest. Browse or search the full list below. ID Plasmid...

...Constitutive or Cre-dependent) Cell-specific restoration of stimulus preference after monocular deprivation...augmented transmembrane chloride movement by gerbil prestin (SLC26a5). PLoS One. 2014 Jun 5;9(6):e99095. Joseph...