...shared experience for the research community and facilitates scientific collaborations between institutions...

...number of plates you’d like to pour. For example: Because we’d like to make 20 plates, and our plates can hold... and negative test results. Sample Data In all cases below (-) indicates that the tested strain is not... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may...Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10...colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of ...appropriate number of plates and stack them on your lab bench. Label the plates with the date and the ...remainder of the plates, pour directly from the bottle. Pro-Tips Be sure to swirl your plates after pouring...

...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from...

...Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies made from...

...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...

... give a 20 minute presentation, followed by 20 minutes of questions/discussion. The goal of the presentation...

...Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar...

... mEGFP CCCTC-binding factor (CTCF) CTCF-binding sites 170818 PCNA-mEGFP AICSDP-116 mEGFP Proliferating...

...Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences...

...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

...colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251...

...your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent ...

... AAV-PR encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic...

...Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234 mEmerald-...

...readings than a DNA sample dissolved in buffer (such as TE). You will get much more accurate and consistent ...ranges from 1.80-2.00). Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop...

...Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA (2013) RNA-guided gene activation by...

...have been used to visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons ... The central nervous system (CNS) orchestrates complex processes enabling organisms to control their ... affects the labeling of small structures like neurites. Like epitope tags, low FP expression may be insufficient...structures through their multiple, high-affinity binding sites for primary antibodies which can be used to amplify...difficult to label. It has also been shown that dendrites in the stratum oriens and the stratum lacunosum...their multiple, highly specific antibody binding sites and primary antibody-based detection, smFPs create...

...digestion and PCR products with sterile water (instead of TE buffer) to ensure optimal salt concentrations in ...room temperature for 30 minutes. Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent ... for 5 minutes at room temperature, then add 1 μl of 25 mM EDTA, followed by another 5 minutes at room...Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-consuming ligation step ...with a single enzyme, and remove the restriction sites from the final product (no "cloning scars"). A “...reaction may be stopped by heating to 75° for 20 minutes. Step 6: Anneal and Transform Mix your treated ...