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We narrowed to 964 results for: tes

Showing: 101 - 120 of 964 results

  1. Distribution to Industry

    Type
    Collection
    ...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from...

  3. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences...

  4. CRISPR Plasmids - Plants

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

  5. Open Enzyme Collection

    Type
    Collection
    ...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top...

  6. Ras Pathway

    Type
    Collection
    ...colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent ...

  9. DNA Quantification

    Type
    Protocol
    ...readings than a DNA sample dissolved in buffer (such as TE). You will get much more accurate and consistent ...ranges from 1.80-2.00). Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop...

  12. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...have been used to visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons ... The central nervous system (CNS) orchestrates complex processes enabling organisms to control their ... affects the labeling of small structures like neurites. Like epitope tags, low FP expression may be insufficient...structures through their multiple, high-affinity binding sites for primary antibodies which can be used to amplify...difficult to label. It has also been shown that dendrites in the stratum oriens and the stratum lacunosum...their multiple, highly specific antibody binding sites and primary antibody-based detection, smFPs create...

  13. Ligation Independent Cloning

    Type
    Protocol
    ...digestion and PCR products with sterile water (instead of TE buffer) to ensure optimal salt concentrations in ...room temperature for 30 minutes. Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent ... for 5 minutes at room temperature, then add 1 μl of 25 mM EDTA, followed by another 5 minutes at room...Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-consuming ligation step ...with a single enzyme, and remove the restriction sites from the final product (no "cloning scars"). A “...reaction may be stopped by heating to 75° for 20 minutes. Step 6: Anneal and Transform Mix your treated ...

  14. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    .... https://doi.org/10.1038/s41592-019-0614-5 Sztal TE, Stainier DYR (2020) Transcriptional adaptation: ...inverted repeats separated by a loop region that facilitates the formation of a hairpin RNA structure, resulting... gene of interest. Tools for RNAi Arguably the greatest advantage of RNAi is the straightforward nature...active development, the first step necessarily dictates the direction and scope of the subsequent two....Artificial micro RNA and short hairpin RNA The greatest limitations of the long hpRNA approach are thought...experiments typically involved antibiotic selection to test for the presence of the RNAi transgene, followed...Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, ...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...mouse)/R. norvegicus (rat) rat IgG2a Addgene distributes ready-to-use recombinant antibodies made from...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...attB sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...reactions. Recombination of attP and attB sites creates attL and attR sites.   Gateway vectors contain modified...takes place between the attB sites flanking the insert and the attP sites of the donor vector. This reaction...enzyme mix and generates the entry clone containing the DNA of interest flanked by attL sites. As a byproduct...reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression clone ... place between the attL sites of the generated entry clone and the attR sites of the destination vector...attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with ...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...and astrocytes Mice No (LY6C1)   Hanlon et al., 2019 AAV-BI-28 CNS: neurons, astrocytes, oligodendrocytes.... It can also effectively transduce astrocytes or oligodendrocytes when paired with appropriate cell type-specific...28 has been tested in C57BL/6J mice and effectively transduces NeuN+ neurons and astrocytes (Huang et al...et al., 2023). Among the capsids tested, AAV9-X1.1 showed the greatest fold change in RNA and DNA levels... others have done, you’ll need to experimentally test your chosen capsid before using it in an application...elements [promoters and enhancers, miRNA target sites, and recombination target sequences, denoted by ...cisterna magna or lumbar spine. Image and caption courtesy of Challis et al., 2022. Where do they come...

  19. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... Cre but lox511 sites can only recombine with other lox511 sites, not with loxP sites. Alternatively, ...at target sites to induce site specific recombination events: Cre recombinase binds loxP sites, while FLP... FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in opposing orientations..., you could use a pair of loxP sites and a pair of FRT sites and include both Cre and FLP recombinases...proceed either by first utilizing the loxP sites or the lox551 sites. Regardless, the first recombination step...fragment using either loxP or lox511 sites, leaving two identical sites on one end of the DNA fragment (Figure..., and that the orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate...

  20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...recombination one of lox2272 sites will now be sandwiched between 2 loxp sites and is excised by Cre (Figure...orientation and one of the unused (loxp) sites will be flanked by two lox sites now in the same orientation. Next...recombination sites, they hypothesized that spontaneous recombination between these sites may be the cause...leaky expression. To test their hypothesis, they randomly scrambled one pair of lox sites and either used ...injection site. Test the promoter It is also essential that the promoter be thoroughly tested in your cells...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest...transgene is floxed by two pairs of recombination sites and the open reading frame is inverted in relation...
Showing: 101 - 120 of 964 results