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We narrowed to 145 results for: transfection

Showing: 101 - 120 of 145 results

  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells Day 7 (am): Harvest cells Equipment Class...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...Polyethylenimine (PEI) 25 kDa MW Pro-Tip Other transfection reagents may be used in this protocol, but their...their conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing ... Pass and plate the cells the day before the transfection. Thaw a new vial of cells after 30 passages....to the incubator for 24 h–36 h. Proceed with transfection: Calculate the amount of each plasmid needed...Note that longer incubation times can reduce transfection efficiency. Add the OptiMEM + DNA + PEI solution...

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Day 2: b. Perform the transfection in the late afternoon because the transfection mix should only be incubated...pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles...transient knockdown of protein expression, you may transfect plasmid DNA directly into the target cells. The...#12259 HEK-293T cells GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001...Producing Lentiviral Particles This protocol is for transfection in a 6 cm plate. The protocol can be scaled ... as needed. Day 1: a. For each plasmid to be transfected, plate 7×105 HEK-293T cells in 5 mL of media ...polystyrene tubes), make a cocktail for each transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging...

  9. Hot Plasmids February 2024

    Type
    Blog Post
    ...electroporation or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem...

  11. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Virus Name Description Link to Video General Transfection Introduce plasmid DNA to mammalian cells Lentivirus...Produce lentivirus with a polyethyenimine (PEI) transfection protocol Fluorescence Titering Assay for Lentivirus...preparation Antibodies Name Description Link to Video Transfection Introduce plasmid DNA to mammalian cells to ...

  12. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... protein expression observed using transient transfection approaches, generating cell lines using lentiviral... stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it eliminates...variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian antibiotic...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers...

  13. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...restriction digest! Then, you can transform or transfect the cells that you want to use for your experiments...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    ...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

  16. DNA Purification Without a Kit

    Type
    Blog Post
    ...could interfere with interpreting the results of transfection assays. Go forth and perform your DNA extractions...

  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...plasmid, and (3) the packaging plasmid. These are transfected into target cells which produce lentiviral particles...
Showing: 101 - 120 of 145 results